引用本文:
刘超超, 韩芸, 卓杨, 白洋, 彭党聪. 城市污水厂剩余污泥中腐殖酸化学组成与结构特征[J]. 环境化学, 2017, 36(8): 1735-1743
LIU Chaochao, HAN Yun, ZHUO Yang, BAI Yang, PENG Dangcong. Chemical composition and structural characteristics of humic acid extracted from excess sludge in municipal wastewater treatment plants[J]. Environmental Chemistry, 2017, 36(8): 1735-1743

城市污水厂剩余污泥中腐殖酸化学组成与结构特征
刘超超, 韩芸, 卓杨, 白洋, 彭党聪
西安建筑科技大学环境与市政工程学院, 西安, 710055
摘要:
采用元素分析、傅里叶红外光谱、固体13C核磁共振以及三维荧光光谱技术,对不同污水处理厂剩余污泥中腐殖酸的化学组成和结构特征进行对比分析.结果表明,不同腐殖酸的化学组成极其相似,但在结构特征上存在明显差异.元素分析计算结果发现,不同腐殖酸的H/C、O/C和C/N的比值存在差异,说明其结构存在差异.荧光光谱显示各个腐殖酸均存在两个明显的特征峰.荧光指数表明各个腐殖酸均为生物源腐殖酸,与元素分析测得的H/C结论一致.傅里叶红外分析显示A厂腐殖酸的各个官能团吸收峰最大、即各个官能团含量最多,C厂含有最少的含氧官能团.13C-NMR结果表明,A厂腐殖酸含有最多的脂肪碳和芳香碳结构,而D厂腐殖酸与之相反.
关键词:    剩余污泥    腐殖酸    元素分析    红外光谱    13C核磁共振    三维荧光光谱    城市污水厂   
Chemical composition and structural characteristics of humic acid extracted from excess sludge in municipal wastewater treatment plants
LIU Chaochao, HAN Yun, ZHUO Yang, BAI Yang, PENG Dangcong
School of Environmental and Municipal Engineering, Xi'an University of Architecture and Technology, Xi'an, 710055, China
Abstract:
Chemical composition and structures of humic acids (HAs) in the excess sludge from different municipal wastewater treatment plants (WWTP) were determined by using elemental analysis, Fourier transformed infrared (FT-IR) spectroscopy, solid-state CP TOSS MAS 13C nuclear magnetic resonance (13C-NMR) spectroscopy and three dimensional fluorescence excitation-emission-matrix (3DEEM) spectroscopy. The results showed that the HAs presented almost the same chemical composition. However, the structures of the HAs were significantly different. The results of elemental analysis showed different HAs had different H/C, O/C, and C/N atomic ratios, which indicated that HAs had different structures. The 3DEEM spectra of all HAs samples presented two characteristic peaks. The fluorescence index showed that all HAs were generated from the biological source, which was consistent with the H/C results of elemental analysis. The results of FT-IR spectra of HAs from plant A showed the maximum absorption peaks for the various functional groups of humic acid. This indicated that the HAs of plant A had the highest content of functional group. While, the HA of plant C contained the least content of oxygen containing functional groups. The results of 13C-NMR indicated that the HA of plant A had the highest content of carbon and aromatic carbon structure compounds, while plant D contained the least content of such structure compounds.
Key words:    excess sludge    humic acids    elemental analysis    fourier transform infrared    13C-NMR    3DEEM    municipal wastewater treatment plant   
收稿日期: 2016-12-29
基金项目: 国家自然科学基金(51408458),国家水体污染控制与治理科技重大专项(2013ZX07315-001-04),陕西省科技统筹创新工程计划项目(2016KTZDSF04-02-02)和陕西省自然科学基金(2017JM5090)资助.
韩芸,Tel:13991207226,E-mail:hanyun@xauat.edu.cn
相关功能
PDF(5117KB) Free
打印本文
加入收藏夹
把本文推荐给朋友
作者相关文章
刘超超  在本刊中的所有文章
韩芸  在本刊中的所有文章
卓杨  在本刊中的所有文章
白洋  在本刊中的所有文章
彭党聪  在本刊中的所有文章

参考文献:
[1] RAMAN L P, CHERYAN M, RAJAGAPALAN N, et al, Nanofiltriatriation for membrane separations[J]. Chemical Engineering Progress,1994,5:68-74.
[2] HWANG S J. Carbon dynamics of plankton communities in nearshore and offshore Lake Erie:The significance of the microbial loop for higher trophic levels[D]. Kent:Kent State University, 1995.
[3] 孙伟亚,何广平,吴宏海,等.珠江河口水体沉积物中腐殖酸的提取与表征[J].应用化工, 2006,35(1):63-66. SUN W Y, HE G P, WU H H, et al. Extraction and characterization of humic acid from estuarine sediment in Zhujiang River[J]. Applied Chemical Industry, 2006,35(1):63-66(in Chinese).
[4] POLAK J, SULKOWSKI W W, BARTOSZEK M, et al. Spectroscopic studies of the progress of humification processes in humic acid extracted from sewage sludge[J]. Journal of Molecular Structure, 2005, 744:983-989.
[5] POLAK J, BARTOSZEK M, SULKOWSKI W W. Comparison of some spectroscopic and physico-chemical properties of humic acids extracted from sewage sludge and bottom sediments[J]. Journal of Molecular Structure, 2009, 924-926:309-312.
[6] 郭瑾, 马军. 松花江水中天然有机物的提取分离与特性表征[J]. 环境科学, 2005, 26(5):77-84. GUO J, MA J. Characterization of isolated fractions of NOM from Songhua River[J]. Environmental Science, 2005, 26(5):77-84(in Chinese).
[7] 田充, 王江涛, 袁秀堂,等海洋沉积物中腐殖酸的提取与紫外吸收光谱特征[J]. 海洋环境科学, 2012,31(6):821-823,829. TIAN C, WANG J T, YUAN X T, et al. Extraction and ultraviolet absorption spectra of humic acid in marine sediment[J]. Marine Environmental Science, 2012,31(6):821-823,829(in Chinese).
[8] 傅平青, 刘丛强, 尹祚莹,等. 腐殖酸三维荧光光谱特性研究[J]. 地球化学, 2004, 33(3):301-308. FU P Q, LIU C Q, YIN Z Y, et al. Characterization of humic acid by three-dimentional excitation emission matrix fluorescence spectroscopy[J].Geochimica, 2004, 33(3):301-308(in Chinese).
[9] 宋海燕, 尹友谊, 宋建中. 不同来源腐殖酸的化学组成与结构研究[J]. 华南师范大学学报(自然科学版), 2009(1):61-66. SONG H Y, YIN Y Y, SONG J Z. The chemical composition and structure of humic acids from different environments[J]. Journal of South China Normal University(Natural Science Edition), 2009(1):61-66(in Chinese).
[10] AMIR S, JOURAIPHY A, MEDDIEH A, et al. Structural study of humic acids during composting of activated sludge-green waste:Elemental analysis, FTIR and 13C NMR[J]. Journal of Hazardous Materials, 2010, 177(1-3):524-529.
[11] SWIFT R S. Organic matter characterization. Methods of soil analysis[M]. Madison:Soil Science Society of America, 1996.
[12] LUX Q, HANNA J V, JOHNSON W D. Source indicators of humic substances:An elemental composition, solid state 13C CP/MAS NMR and Py-GC/MS Study[J]. Applied Geochemistry, 2000, 15(7):1019-1033.
[13] MEYERS P A, ISHIWATARI R. Lacustrine organic geochemistry-An overview of indicators of organic matter sources and diagenesis in lake sediments[J]. Organic Geochemistry, 1993, 20(7):867-900.
[14] NELSON P N, DICTOR M C, SOULAS G. Availability of organic carbon in soluble and particle-size fractions from a soil profile[J]. Soil Biology & Biochemistry, 1994, 26(11):1549-1555.
[15] 李会杰. 腐殖酸和富里酸的提取与表征研究[D]. 武汉:华中科技大学, 2012. LI H J. Study on extraction and characterization of HA and FA[D]. Wuhan:Huazhong University of Science and Technology,2012(in Chinese).
[16] STEVENSON F J. Humus chemistry:genesis, composition, reactions[M]. 2nd ed. New York:John Wiley & Sons,1994.
[17] 张耀玲. 近海环境中天然有机质的分离与表征[D]. 上海:华东师范大学, 2013. ZHANG Y L. Isolation and characterization of natural organic matter in coastal environments[D]. Shanghai:East China Normal University, 2013(in Chinese).
[18] PAULA G Coble. Characterization of marine and terrestrial DOM in seawater using excitation-emission matrix spectroscopy[J]. Marine Chemistry, 1996, 51(4):325-346.
[19] SIERRA M M D, GIOVANELA M, PARLANTI E, et al. Fluorescence fingerprint of fulvic and humic acids from varied origins as viewed by single-scan and excitation/emission matrix techniques[J]. Chemosphere, 2005, 58(6):715-733.
[20] PARLANTI E, WORZ K, GEOFFROY L, et al. Dissolved organic matter fluorescence spectroscopy as a tool to estimate biological activity in a coastal zone submitted to anthropogenic inputs[J].Organic Geochemistry,2000, 31(12):1765-1781.
[21] PATEL-SORRENTINO N, MOUNIER S, BENAIM J Y. Excitation-emission fluorescence matrix to study pH influence on organic matter fluorescence in the Amazon basin rivers.[J]. Water Research, 2002, 36(10):2571-2581.
[22] 罗固源, 朱虹, 许晓毅,等. 嘉陵江重庆段沉积物中腐殖酸的特性研究[J]. 光谱学与光谱分析, 2011, 31(6):1663-1667. LUO G Y, ZHU H, XU X Y, et al. Studies on characterization of humic acids extracted from sediments in Chongqing section of the Jialingjiang River[J]. Spectroscopy and Spectral Analysis, 2011, 31(6):1663-1667(in Chinese).
[23] AMINIYAN M M, SINRGANI A A S, SHEKLABADI M. The effect of applied nanozeolite and some plant residues on organic carbon changes in density and soluble fractions-incubation study[J]. Agricultural Advances, 2014, 3(11):280-291.
[24] GHABBOUR E A, KHAIRY A H, CHENEY D P, et al. Isolation of humic acid from the brown alga Pilayella littoralis[J]. Journal of Applied Phycology, 1994, 6(5):459-468.
[25] MATTHEWS B J H, JONES A C, THEODOROU N K, et al. Excitation-emission-matrix fluorescence spectroscopy applied to humic acid bands in coral reefs[J]. Marine Chemistry, 1996, 55(3/4):317-332.
[26] MCKNIGHT D M, BOYER E W, WESTERHOFF P K, et al. Spectrophotometric characterization of dissolved organic matter for indication of precursor organic material and aromaticity[J]. Limnology & Oceanography, 2001, 46(1):38-48.
相关文献:
1.王晓慧, 刘永军, 刘喆, 邢超, 杨月乔.用三维荧光和红外技术分析好氧颗粒污泥形成初期胞外聚合物的变化[J]. 环境化学, 2016,35(1): 125-132