引用本文:
张芳, 郜红建, 葛高飞. 苯并[a]芘累积污染对土壤微生物群落功能多样性的影响[J]. 环境化学, 2017, 36(8): 1849-1857
ZHANG Fang, GAO Hongjian, GE Gaofei. Effects of cumulative benzo(a)pyrene pollution on functional diversity of microbial community in soil[J]. Environmental Chemistry, 2017, 36(8): 1849-1857

苯并[a]芘累积污染对土壤微生物群落功能多样性的影响
张芳1,2, 郜红建1, 葛高飞2
1. 安徽农业大学资源与环境学院, 合肥, 230036;
2. 安徽农业大学生物技术中心, 合肥, 230036
摘要:
本文采用低剂量叠加的方式模拟苯并[a]芘(Benzo(a)pyrene,BaP)在实际土壤中的累积过程,利用Biolog-ECO方法研究BaP不同污染方式对土壤微生物群落功能的影响,以期揭示BaP累积污染对土壤微生物群落功能多样性的影响.结果表明,在累积和一次污染方式下,土壤BaP可提取态和有效态含量随土壤培养时间的延长而降低,前期(1-28 d)下降速率较快,后期(28-56 d)下降速率减缓.土壤微生物代谢活性AWCD值在培养初期(1 d)表现出L > CK-Y > Y > CK-L的规律,培养7 d表现为Y > CK-Y > CK-L > L,而后(8-56 d)各处理的AWCD值表现与培养7 d的规律一致.微生物群落物种丰富度指数和常见物种优势度指数的变化趋势与AWCD值变化规律一致.累积污染处理在土壤培养过程中先促进(1 d)后抑制(7-56 d)了土壤微生物群落功能多样性;一次污染处理与之相反,先抑制(1 d)后刺激(7-56 d)了土壤微生物群落功能多样性.累积污染处理的微生物代谢活性和多样性指数与BaP含量的相关性大于一次污染.累积污染更能反映BaP在土壤中的真实生物毒性.
关键词:    BaP    累积污染    Biolog-ECO    土壤微生物    群落功能多样性   
Effects of cumulative benzo(a)pyrene pollution on functional diversity of microbial community in soil
ZHANG Fang1,2, GAO Hongjian1, GE Gaofei2
1 School of Resources and Environment, Anhui Agricultural University, Hefei, 230036, China;
2 Biotechnology Center, Anhui Agricultural University, Hefei, 230036, China
Abstract:
To reveal the impact of accumulated pollution of Benzo(a)pyrene (BaP) on the functional diversity of soil microbial community, BaP was added to soil by low-dose superposition method to simulate the cumulative pollution of BaP in natural soil, and the effects of different BaP pollution on microbial community functions in soil was investigated by the Biolog-ECO method. The results showed that the extractble and available BaP contents in soil declined quickly in the first 28 days and then decreased slowly from 28 to 56 days under the one-time and accumulated pollution conditions. AWCD, reflecting the soil microbial metabolic activity, followed the order of L > CK-Y > Y > CK-L at the beginning of incubation period (1 d), and Y > CK-Y > CK-L > L on the 7th day. Then the order of AWCD during the rest of incubation period (8-56 d) was the same as that on the 7th day. Moreover, Shannon and Simpson index showed similar patterns as the AWCD under the one-time and accumulated pollution conditions. Compared with CK-L and CK-Y, accumulated pollution promoted the functional diversity (1 d) and then inhibited microbial activities (7-56 d). One-time pollution treatment inhibited the functional diversity (1 d) and then stimulated microbial activities (7-56 d). Besides, there was higher positive correlation between the contents of BaP and microbial community functional diversity index in the accumulated pollution soil than in one-time pollution soil. The low-dose superposition method is more appropriate to reflect environmental risk of BaP in soil.
Key words:    BaP    accumulated pollution    Biolog-ECO    soil microorganism    functional diversity   
收稿日期: 2016-12-30
基金项目: 国家自然科学基金青年基金(41401278)资助.
葛高飞,Tel:0551-65785847,E-mail:gegaofei@ahau.edu.cn
相关功能
PDF(2117KB) Free
打印本文
加入收藏夹
把本文推荐给朋友
作者相关文章
张芳  在本刊中的所有文章
郜红建  在本刊中的所有文章
葛高飞  在本刊中的所有文章

参考文献:
[1] QIAO M, CHEN Y, WANG C X, et al. DNA damage and repair process in earthworm after in-vivo and in vitro exposure to soil irrigated by wastewaters[J]. Environment Pollution, 2007, 148(1):141-147.
[2] 陶玉强,张淑贞,薛滨.土壤中多环芳烃生物有效性及其评价方法[J].中国科学院研究生院学报, 2010, 27(4):568-576. TAO Y Q,ZHANG S Z,XUE B. Bioavailability of polycyclic aromatic hydrocarbons in soils and its evaluation method[J]. Journal of the Graduate School of the Chinese Academy of Sciences, 2010, 27(4):568-576(in Chinese).
[3] 刘世亮,骆永明,吴龙华,等.污染土壤中苯并[a]芘的微生物共代谢修复研究[[J].土壤学报,2010, 47(2):364-369. LIU S L, LUO Y M, WU L H, et al. Remediation of benzo[a]pyrene-contaminated soil through its co-metabolism with soil microbes[J]. Acta Pedologica Sinica,2010, 47(2):364-369(in Chinese).
[4] 刘君. 苯并芘污染土壤的微生物效应及其降解菌特性的研究[D].呼和浩特:内蒙古大学, 2008. LIU J. The effects of benzopyrene on soil microorganisms and the reaserch of degradation condations of benzopyrene-degrading bacteria[D]. Huhehaote:Inner Moncolia University, 2008(in Chinese).
[5] 臧淑艳,李培军,张英,等.污染土壤中苯并(a)芘的微生物降解途径研究进展[J]. 生态学杂志, 2006, 25(8):978-982. ZANG S Y, LI P J, ZHANG Y, et al. Research advances in microbial metabolic pathway of benzo[a]pyrene in contaminated soils[J]. Chinese Journal of ecology, 2006, 25(8):978-982(in Chinese).
[6] 李培军,许华夏,张春桂,等.污染土壤中苯并(a)芘的微生物降解[J].环境污染治理技术与设备,2001, 2(5):37-40. LI P J, XU H X, ZHANG C G, et al. The degradation of B(a)P by microorganism in contaminated soil[J]. Techniques and Equipment for Environmental Pollution Control, 2001,2(5):37-40(in Chinese).
[7] 杨翠云,郭淑政,刘琪,等. 石油污染土壤微生物多样性的研究技术及进展[J]. 安徽农业科学,2009, 37(33):16479-16482,16533. YANG C Y, GUO S Z, LIU Q, et al. Research techniques and progress of oil-contaminated soil microbial diversity[J]. Jouranl of Anhui Agricultural Sciences, 2009,37(33):16479-16482, 16533(in Chinese).
[8] O'DONNELL A G, SEASMAN M, MACRAE A, et al. Plants and fertilisers as drivers of change in microbial community structure and function in soils[J]. Plant and Soil, 2001, 232(1):135-145.
[9] BAUHUS J, KHANNA P K. Carbon and nitrogen turnover in two acid forest soils of southeast Australia as affected by phosphorus addition and drying and rewetting[J]. Biology and Fertility of Soils, 1994, 17:212-218.
[10] WOLTERS V, JOERGENSEN R G. Microbial carbon turnover in beaeh forest soils at different stages of acidification[J]. Soil Biology and Biochemistry, 1991, 23:897-902.
[11] WARDLE D A. A comparative assessment of factors which influence microbial biomass carbon and nitrogen levels in soils[J]. Biological Reviews, 1992, 67:321-358.
[12] 张晶,张惠文,李新宇,等. 多环芳烃胁迫下稻田土壤细菌及分支杆菌种群多样性研究[J]. 中国生态农业学报, 2008, 16(2):405-410. ZHANG J, ZHANG H W, LI X Y, et al. Bacterium and mycobacterium diversity in paddy soils under PAHs stress[J]. Chiness Journal of Eco-Agriculture, 2008, 16(2):405-410(in Chinese).
[13] 马静静,张伟,郑彬,等.土壤BaP多次叠加污染对蚯蚓体腔细胞的毒性效应[J].南京农业大学学报,2014,37(3):77-82. MA J J, ZHANG W, ZHENG B, et al. Multiple-time addition of benzo[a]pyrene in soil declined its toxic effects on Eisenia foetida coelomocytes[J]. Journal of Nanjing Agricultural University, 2014, 37(3):77-82(in Chinese).
[14] 郑彬,马静静,葛高飞,等. BaP累积污染对土壤呼吸强度的影响[J]. 农业环境科学学报,2013, 32(2):259-266. ZHENG B, M A JJ, GE G F, et al. Influences of benzo(a)pyrene superimposed pollution on soil respiration intensity[J]. Journal of Agro-Environment Science, 2013, 32(2):259-266(in Chinese).
[15] 吕正勇,杨兴伦,王芳,等. Tenax提取预测老化土壤中多环芳烃的生物有效性[J]. 中国环境科学,2011, 31(4):647-656. LU Z Y, YANG X L, WANG F, et al. The tenax extraction technique to predict the bioavailability of aged PAHs in soil[J]. China Environmental Science, 2011, 31(4):647-656(in Chinese).
[16] GARLAND J L, MILLS A L. Classification and characterization of heterotrophic microbial communities on the basis of patterns of community-level sole-carbon-source utilization[J]. Applied and Environmental Microbiology,1991, 57:2351-2359.
[17] CLASSEN A T, BOYLE S I, HASKINS K E, et al. Community level physiological profiles of bacteria and fungi:Plate type and incubation temperature influences on contrasting soils[J]. FEMS Microbiology Ecology, 2003, 44:319-328.
[18] 张伟,郑彬,马静静,等. 土壤BaP多次叠加污染的生物有效性及对蚯蚓体腔细胞染色体和溶酶体的毒性效应[J]. 应用与环境生物学报, 2014, 20(6):1020-1026. ZHANG W, ZHENG B, MA J J, et al. Effect of multiple benzo(a)pyrene addition on its bioavailability and coelomocyte lysosomes and chromosomes in earthworms[J]. Chin J Appl Environ Biol, 2014, 20(6):1020-1026(in Chinese).
[19] YANG Y L, ZHANG N, XUE M, et al.Impact of soil organic matter on the distribution of polycyclic aromatic hydrocarbons (PAHs) in soils[J]. Environmental Pollution,2010, 158(6):2170-2174.
[20] LING W T, ZENG Y C, GAO Y Z, et al.Availability of polycyclic aromatic hydrocarbons in aging soils[J]. Journal of Soils and Sediments, 2010, 10:799-807.
[21] 宫璇,李培军,张海荣,等. 菲对土壤酶活性的影响[J].农业环境科学学报,2004,23(5):981-984. GONG X, LI PJ, ZHANG HR, et al. Effects of phenanthrene contamination on enzyme activity in soil[J]. Journal of Agro-Environment Science, 2004, 23(5):981-984(in Chinese).
[22] SHEN G Q, LU Y T, ZHOU Q X, et al. Interaction of polycyclic aromatic hydrocarbons and heavy metals on soil enzyme[J]. Chemosphere, 2005, 61(8):1175-1182.
[23] 张晶,张惠文,丛峰,等.长期灌溉含多环芳烃污水对稻田土壤酶活性与微生物种群数量的影响[J]. 生态学杂志, 2007,26(8):1193-1198. ZHANG J, ZHANG H W, CONG F, et al. Effects of long-term PAHs-containing wastewater irrigation on lowland rice soil enzyme activities and microbial populations[J]. Chinese Journal of Ecology, 2007, 26(8):1193-1198(in Chinese).
[24] 张晶,张惠文,张勤,等. 长期石油污水灌溉对东北旱田土壤微生物生物量及土壤酶活性的影响[J].中国生态农业学报, 2008, 16(1):67-70. ZHANG J, ZHANG H W, ZHANG Q, et al. Impact of long-time irrigation of petroleum wastewater on glebe microbial biomass and soil enyme activities in Northeast China[J]. Chiness Journal of Eco-Agriculture, 2008, 16(1):67-70(in Chinese).
[25] 沈国清,陆贻通,洪静波,等.菲和镉复合污染对土壤微生物的生态毒理效应[J].环境化学, 2005, 24(6):662-665. SHEN G Q, LU Y T, HONG J B, et al. ECO toxicological effect of phenanthrene and Cd combined pollution on soil micuobe[J]. Environmental Chemistry, 2005, 24(6):662-665(in Chinese).
[26] 易志刚. 多环芳烃对土壤线虫和微生物生物量的影响[J]. 亚热带农业研究, 2008,4(2):141-145. YI Z G. Effects of polycyclic aromatic hydrocarbons on soil nematodes and soil microbial biomass[J]. Subtropical Agriculture Research, 2008,4(2):141-145(in Chinese).
[27] 郑彬,马静静,葛高飞,等. 菲累积污染对其生物有效性特征及土壤呼吸强度的影响[J]. 环境化学,2013,32(12):2342-2348. ZHENG B, MA J J, GE G F, et al. Influences of phenanthrene superimposed pollution on it's bioavailability characteristics and soil respiration intensity. Environmental Chemistry, 2013, 32(12):2342-2348(in Chinese).
[28] 姜睿玲,杨统一,唐玉斌,等. 多环芳烃污染对桑园土壤微生物结构及种群多样性的影响[J]. 中国环境科学,2012,32(9):1655-1661. JIANG R L, YANG T Y, TANG Y B, et al. Effect of PAHs pollution on microbial structure and population diversity of mulberry orchard soil[J]. China Environmental Science, 2012, 32(9):1655-1661(in Chinese).