引用本文:
黄梦璐, 张祯, 吴向阳. 时间分辨荧光免疫法检测环境中四溴双酚A衍生物(TBBPA DHEE)[J]. 环境化学, 2018, 37(10): 2124-2130
HUANG Menglu, ZHANG Zhen, WU Xiangyang. A sensitive time-resolved fluoroimmunoassay for the detertion of tetrabromobisphenol A bis(2-hydroxyetyl) ether in environments[J]. Environmental Chemistry, 2018, 37(10): 2124-2130

时间分辨荧光免疫法检测环境中四溴双酚A衍生物(TBBPA DHEE)
黄梦璐, 张祯, 吴向阳
江苏大学环境与安全工程学院, 镇江, 212013
摘要:
本研究利用前期制备的四溴双酚A双(2-羟基乙基)醚(TBBPA DHEE)的多克隆抗体,构建了一种高灵敏的时间分辨荧光免疫分析法,用于检测环境中的四溴双酚A衍生物TBBPA DHEE.为提高分析方法的灵敏度,本研究对反应体系中有机溶剂(甲醇)、离子强度及缓冲体系pH等参数进行了优化.在最适条件下,本方法的最低检测限(LOD,基于F/F0=90%)与IC50分别为0.27 ng·mL-1及4.3 ng·mL-1.该方法具有较高的准确度(水样加标回收率为96%-120%;土样及生物加标回收率为75%-90%).利用该方法对江苏省苏州某地区的环境样品进行了调查:在采集的56个样本中,13个样本检出了这种污染物.检出浓度为:水样0.5-2.7 ng·mL-1、土样0.6-1.6 ng·g-1,干重、生物样2.9-4.6 ng·g-1,湿重.本研究为首次针对环境中TBBPA DHEE污染状况的系统性分析.
关键词:    时间分辨荧光免疫法    多克隆抗体    四溴双酚A衍生物    四溴双酚A双(2-羟基乙基)醚    环境污染调查   
A sensitive time-resolved fluoroimmunoassay for the detertion of tetrabromobisphenol A bis(2-hydroxyetyl) ether in environments
HUANG Menglu, ZHANG Zhen, WU Xiangyang
School of the Environment and Safety Engineering, Jiangsu University, Zhenjiang, 212013, China
Abstract:
A sensitive time-resolved fluoroimmunoassay (TRFIA) was developed using our previously produced polyclonal antibody for the determination of tetrabromobisphenol A bis(2-hydroxyetyl) ether (TBBPA DHEE), one of typical tetrabromobisphenol A (TBBPA) derivatives. The established method showed satisfactory sensitivity under the optimized conditions. The detection limit (LOD, based on 90% F/F0) and IC50 calculated from the standard curve were 0.27 and 4.3 ng·mL-1 for TBBPA DHEE, respectively. Using the TRFIA method, a systematic investigation was implemented at a halogenated flame retardants plant (main sampling site) in Suzhou, Jiangsu province, China. As a result, TBBPA DHEE was found in 5 of the 26 water samples with concentrations of 0.5-2.7 ng·mL-1, 5 of 20 soil samples with concentrations of 0.6-1.6 ng·g-1 (dry weight, dw), and 3 out of 10 fist samples with concentrations of 2.9-4.6 ng·g-1 (wet weight, ww). To our knowledge, this is the systematic investigation of TBBPA DHEE about its occurrence in the environment.
Key words:    time-resolved fluoroimmunoassay (TRFIA)    immunoassay    tetrabromobisphenol A (TBBPA) derivatives    tetrabromobisphenol A bis (2-hydroxyetyl) ether    environmental occurrence   
收稿日期: 2017-12-27
基金项目: 国家自然科学基金(21577051)资助.
吴向阳,Tel:0511-88791798,E-mail:wuxy@ujs.edu.cn
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