引用本文:
谭凯婷, 王志红, 黄梓淇, 仇永婷, 柳君侠. 对叔丁基邻苯二酚对铜绿微囊藻的化感抑制作用[J]. 环境化学, 2018, 37(12): 2645-2650
TAN Kaiting, WANG Zhihong, HUANG Ziqi, QIU Yongting, LIU Junxia. Allelopathic Inhibition of p-tert-butylcatechol(TBC) on Microcystis aeruginosa[J]. Environmental Chemistry, 2018, 37(12): 2645-2650

对叔丁基邻苯二酚对铜绿微囊藻的化感抑制作用
谭凯婷, 王志红, 黄梓淇, 仇永婷, 柳君侠
广东工业大学土木与交通工程学院, 广州, 510006
摘要:
利用藻种群竞争机制和藻间化感作用,筛选化感物质作为除藻功能材料是藻华控制技术近年来的研究热点.研究利用课题组前期工作成果,从藻滤液中甄别出化感物质对叔丁基邻苯二酚(TBC),在此基础上针对典型蓝藻铜绿微囊藻,进行了系列药剂抑藻实验.通过研究发现,与邻苯二酚及龙胆酸相比,TBC抑制铜绿微囊藻生长的效果更好.在初始藻密度为1×106 cell·mL-1条件下,TBC投加浓度为0.05 mg·L-1时,短时间内可对铜绿微囊藻起到显著的抑制作用,0.1 mg·L-1的投加浓度则可维持15 d不复发.当初始藻密度为5×105-5×106 cell·mL-1时,投加0.1 mg·L-1 TBC能在15 d达到90%以上的抑制率,且藻生物量9 d内不反弹.在pH 6.5-8.5范围,TBC具有较好且持久的抑藻效果.在25-35℃下,TBC对铜绿微囊藻都具有较好的抑制效果,其中25℃抑制效果好于35℃.
关键词:    对叔丁基邻苯二酚    铜绿微囊藻    化感抑制   
Allelopathic Inhibition of p-tert-butylcatechol(TBC) on Microcystis aeruginosa
TAN Kaiting, WANG Zhihong, HUANG Ziqi, QIU Yongting, LIU Junxia
School of Civil and Transportation Engineering, Guangdong University of Technology, Guangzhou, 510006, China
Abstract:
Using algal population competition and algal allelopathy to screen allelochemicals as algae-removing materials is a research hotspot of algal bloom control technology in recent years. Based on the previous results that on allelochemical p-tert-butylcatechol(TBC) was identified from algae filtrate, chemical inhibit tests on cyanobacteria Microcystis aeruginosa(M. aeruginosa) were conducted. The experimental results showed that TBC had better suppression on the growth of M. aeruginosa than catechol and gentisic acid. The growth of M. aeruginosa whose initial density was 1×106 cell·mL-1 was significantly inhibited after exposure to 0.05 mg·L-1 TBC in a short time. When the concentration of TBC was 0.1 mg·L-1, it could maintain 15 d, without relapse. When the initial density of algae was 5×105-5×106 cell·mL-1, the relative inhibition rate of algae could reach more than 90% after 15 d and the algae biomass did not rebound within 9 d. TBC has a good and persistent inhibitory effect on M. aeruginosa when the pH value was between 6.5 and 8.5. It was effectively inhibited in the temperature range of 25-35℃ with the optimum temperature of 25℃.
Key words:    p-tert-butylcatechol    Microcystis aeruginosa    allelopathic inhibition   
收稿日期: 2018-05-06
基金项目: 国家自然科学基金(51708130,51308131)资助.
王志红,Tel:18929506642,E-mail:gdwzhihong@126.com
相关功能
PDF(KB) Free
打印本文
加入收藏夹
把本文推荐给朋友
作者相关文章
谭凯婷  在本刊中的所有文章
王志红  在本刊中的所有文章
黄梓淇  在本刊中的所有文章
仇永婷  在本刊中的所有文章
柳君侠  在本刊中的所有文章

参考文献:
[1] PAERL H W, XU H, MCCARTHY M J, et al. Controlling harmful cyanobacterial blooms in a hyper-eutrophic lake (Lake Taihu, China):the need for a dual nutrient (N & P) management strategy[J]. Water Research, 2011, 45(5):1973-1983.
[2] ZHANG T T. The allelopathy and allelopathic mechanism of phenolic acids on toxic Microcystis aeruginosa[J]. Journal of Applied Phycology, 2010, 22(1):71-77.
[3] NI L X, ACHARYA K, MAO X Y, et al. Isolation and identification of an anti-algal compound from Artemisia annua and mechanisms of inhibitory effect on algae[J]. Chemosphere, 2012, 88(9):1051-1057.
[4] VYVYAN J R. ChemInform Abstract:Allelochemicals as leads for new herbicides and agrochemicals[J]. Cheminform, 2002, 58(9):1631-1646.
[5] NAKAI S, INOUE Y, HOSOMI M. Algal growth inhibition effects and inducement modes by plant-producing phenols[J]. Water Research, 2001, 35(7):1855-1859.
[6] NAKAI S, INOUE Y, HOSOMI M, et al. Myriophyllum spicatum -released allelopathic polyphenols inhibiting growth of blue-green algae Microcystis aeruginosa[J]. Water Research, 2000, 34(11):3026-3032.
[7] NAKAI S, YAMADA S, HOSOMI M. Anti-cyanobacterial fatty acids released from Myriophyllum spicatum[J]. Hydrobiologia, 2005, 543(1):71-78.
[8] 王红强, 成水平, 张胜花, 等. 伊乐藻生物碱的GC-MS分析及其对铜绿微囊藻的化感作用[J]. 水生生物学报, 2010, 34(2):361-366. WANG H Q, CHENG S P, ZHANG S H, et al. Analysis of alkaloid from elodea nuttallii by GC-MS and its allelopathic activity on microcystis aeruginosa[J]. Acta Hydrobiologica Sinica, 2010, 34(2):361-366(in Chinese).
[9] ZI J C, MAFU S, PETERS R J. To gibberellins and beyond! surveying the evolution of (Di) terpenoid metabolism[J]. Annual Review of Plant Biology, 2014, 65(1):259-286.
[10] WESTON L A, MATHESIUS U. Flavonoids:Their structure, biosynthesis and role in the rhizosphere, including allelopathy[J]. Journal of Chemical Ecology, 2013, 39(2):283-297.
[11] KORNER S. Loss of submerged macrophytes in shallow lakes in north-eastern germany[J]. International Review of Hydrobiology, 2002, 87(4):375-384.
[12] 倪利晓, 任高翔, 陈世金, 等. 酚酸和不饱和脂肪酸对铜绿微囊藻的联合作用[J]. 环境化学, 2011, 30(8):1428-1432. NI L X, REN G X, CHEN S J, et al. Study on joint action of phenolic acids and unsaturated fatty acids to Microcystis aeruginosa[J]. Environmental Chemistry, 2011, 30(8):1428-1432(in Chinese).
[13] LU Z Y, LIU B Y, HE Y, et al. Effects of daily exposure of cyanobacterium and chlorophyte to low-doses of pyrogallol[J]. Allelopathy Journal, 2014, 34(2):195-205.
[14] QIU Y T, LIU F, WANG Z H, et al. Allelopathic effect of scenedesmus on microcystis flos-aquae//Proceedings of Energy and Chemical Engineering of the 20176th International Conference on Environment, Chemistry and Biology[C]. Australia:IACSIT Press, 2017:103-108.
[15] 李锋民, 胡洪营. 植物化感作用控制天然水体中有害藻类的机理与应用[J]. 给水排水, 2004, 30(2):1-4. LI F M, HU H H. Mechanism of phyto-allelochemicals and its application for harmful algae control in nature water body[J]. Water & Wastewater Engineering, 2004, 30(2):1-4(in Chinese).
[16] 高磊, 张鸿涛, 范婧, 等. 3种药剂对普通小球藻的抑制作用[J]. 环境工程学报, 2013, 7(12):4869-4873. GAO L, ZHANG H T, FAN J, et al. Inhibitional effect of 3 chemical substances on chlorella[J]. Chinese Journal of Environmental Engineering, 2013, 7(12):4869-4873(in Chinese).
[17] 郭亚丽, 傅海燕, 黄国和, 等. 阿魏酸和香豆素对铜绿微囊藻的化感作用[J]. 环境科学, 2013, 34(4):1492-1497. GUO Y L, FU H Y, HUANG G H, et al. Allelopathy effects of ferulic acid and coumarin on microcystis aeruginosa[J]. Environmental Science, 2013, 34(4):1492-1497(in Chinese).
[18] 张庭廷, 吴安平, 何梅, 等. 酚酸类物质对水华藻类的化感作用及其机理[J]. 中国环境科学, 2007, 27(4):472-476. ZHANG T T, WU A P, HE M, et al. The allelopathy and its mechanism of phenolic acids on water-bloom algae[J]. China Environmental Science, 2007, 27(4):472-476(in Chinese).
[19] 丁惠君, 张维昊, 周伟斌, 等. 两种酚酸类化感物质对铜绿微囊藻生长的影响[J]. 环境科学与技术, 2007, 30(7):1-3, 115. DING H J, ZHAG W H, ZHOU W B, et al. Study on growth of microcystis aeruginosa affected by two phenolic allelochemicals[J]. Environmental Science & Technology, 2007, 30(7):1-3, 115(in Chinese).
[20] WANG J X, XIE P, GUO N C. Effects of nonylphenol on the growth and microcystin production of Microcystis strains[J]. Environmental Research, 2007, 103(1):70-78.
[21] HONG Y, HU H Y, LI F M. Physiological and biochemical effects of allelochemical ethyl 2-methyl acetoacetate (EMA) on cyanobacterium Microcystis aeruginosa[J]. Ecotoxicol Environ Saf, 2008, 71(2):527-534.
[22] POULSON K L, SIEG R D, PRINCE E K, et al. Allelopathic compounds of a red tide dinoflagellate have species-specific and context-dependent impacts on phytoplankton[J]. Marine Ecology Progress, 2010, 416(6):69-78.
[23] DAN J, HUANG L F, LIN S Q, et al. Allelopathic effects of euhalophyte Salicornia bigelovii on marine alga Skeletonema costatum[J]. Allelopathy Journal, 2010, 25(1):163-172.
[24] 冯鸣凤. 不同环境因子条件下对硝基酚对藻类的毒性研究[D]. 天津:南开大学, 2010. FENG M F. The toxicity of p-Nitrophenol on algae under different environmental conditions[D]. Tianjin:Nankai University, 2010(in Chinese).