引用本文:
李丽红, 刘兴国. 采用电雾式检测器测定掺假蜂蜜中的果葡糖浆[J]. 环境化学, 2018, 37(12): 2824-2826
LI Lihong, LIU Xingguo. Determination of fructose-glucose syrup in adulterated honey by HPLC-CAD[J]. Environmental Chemistry, 2018, 37(12): 2824-2826

采用电雾式检测器测定掺假蜂蜜中的果葡糖浆
李丽红1, 刘兴国2
1. 华北制药集团新药研究开发有限责任公司 微生物药物国家研究工程中心 河北省工业微生物代谢工程技术研究中心, 石家庄, 050015;
2. 赛默飞世尔科技(中国)有限公司, 北京, 100080
摘要:
采用电雾式检测器建立了鉴别掺假蜂蜜的方法,实验采用乙腈和水(65:35)流动相等度洗脱,流速1.2 mL·min-1,Shodex Asahipak NH2P-50 4E色谱柱(4.6×150 mm,5 μm)进行分离,Corona Veo RS检测器采集频率5 Hz,蒸发温度35℃,Filter5.不同聚合度的麦芽糖在8.0-60.0 μg·mL-1浓度范围,线性相关系数为0.999;方法灵敏度高,1%果葡糖浆可明显检出.方法快捷高效,可用于掺假蜂蜜的快速鉴别.
关键词:    电雾式检测器    掺假蜂蜜    果葡糖浆    麦芽糖   
Determination of fructose-glucose syrup in adulterated honey by HPLC-CAD
LI Lihong1, LIU Xingguo2
1. New Drug Research and Development Center of North China Pharmaceutical Group Corporation, National Microbial Medicine Engineering and Research Center, Hebei industry Microbial Metabolic Engineering & technology Research Center, Shijiazhuang, 050015, China;
2. Thermofisher Scientific(China) Co., Ltd, Beijing, 100080, China
Abstract:
A HPLC-CAD Method was established for rapidly quantification of oligosaccharides adulterated honey. We use a Shodex Asahipak NH2P-50 4E column(4.6×150 mm, 5 μ m)to seperate these compounds, with the mobile phase consisting of acetonitrile and H2O (65:35). The samples were detected by Corona Veo RS detector, with collect rate 5Hz,evaporative temperature 35℃,Filter 5. As a result, using the chromatographic peaks of maltooligosaccharide (maltose to maltoheptaose) standards as reference, the differences of honey, fructose-glucose syrup and pure honey containing fructose-glucose syrup were detected by CAD. The linear ranges of these different malto oligosaccharides were 8.0-60.0 mg·L-1 and the correlation coefficients(r) were all 0.999. 1% fructose-glucose syrup in adulterated honey can be detected, this method showed high accuracy, sensitivity and short time for analysis.
Key words:    HPLC-CAD    adulterated honey    fructose-glucose syrup    maltose   
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刘兴国,E-mail:734814659@qq.com
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