引用本文:
王建宇, 李丽, 王蕴平, 王桂芳, 袁汉成, 洪云鹤, 孙颖. 在线固相萃取-超高效液相色谱法检测水中15种多环芳烃类污染物[J]. 环境化学, 2018, 37(12): 2832-2836
WANG Jianyu, LI Li, WANG Yunping, WANG Guifang, YUAN Hancheng, HONG Yunhe, SUN Ying. 15 kinds of polycyclic aromatic hydrocarbons in water were detected by solid phase extraction and ultra-high performance liquid chromatography[J]. Environmental Chemistry, 2018, 37(12): 2832-2836

在线固相萃取-超高效液相色谱法检测水中15种多环芳烃类污染物
王建宇1, 李丽1, 王蕴平1, 王桂芳1, 袁汉成2, 洪云鹤2, 孙颖1
1. 北京市水文地质工程地质大队, 北京, 100195;
2. 沃特世科技(上海)有限公司, 北京, 100026
摘要:
由于传统固相萃取需耗费大量时间,使用很多有机溶剂,在样品量多的时候,会出现大量样品滞留,为提高实验室工作人员的工作效率,本文建立了在线固相萃取-超高效液相色谱法测定水中15种多环芳烃污染物的方法.水样经在线固相萃取小柱富集,高效液相色谱柱分离,乙腈和水溶液梯度洗脱,荧光检测器检测,外标法定量.在进样量为2 mL时,萘、二氢苊、芴、菲、蒽、荧蒽、芘、(口恶)、苯并(a)蒽、苯并(b)荧蒽、苯并(k)荧蒽、苯并(a)芘、二苯并(a,h)蒽、苯并(g,h,i)苝、茚并(1,2,3-cd)芘在5-80 ng·L-1范围内均具有良好的线性关系.实际水样回收率均大于70%,此方法可用于水中15种多环芳烃类污染物的检测.
关键词:    多环芳烃    在线固相萃取    超高效液相色谱仪   
15 kinds of polycyclic aromatic hydrocarbons in water were detected by solid phase extraction and ultra-high performance liquid chromatography
WANG Jianyu1, LI Li1, WANG Yunping1, WANG Guifang1, YUAN Hancheng2, HONG Yunhe2, SUN Ying1
1. Beijing Institute of Hydrogeology and Engineering Geology, Beijing, 100195, China;
2. Waters Technologies(Shanghai) Limited, Beijing, 100026, China
Abstract:
The traditional solid-phase extraction has many disadvantages. It alwaystakes a lot of time and uses too many organic solvents. Besides that, when there are a large number of samples need to be solid-phase extraction, most of them will have the phenomenon of retention.To improve the working efficiency of the staff in laboratory,we provide a new technology of on-line solid phase extraction. This method can test 15 polycyclic aromatic hydrocarbon(PAHs) pollutants in water.The water is enriched by on-line solid phase extraction column, high performance liquid chromatography column separation, acetonitrile and aqueous solution gradient elution, fluorescence detector detection, and external standard method quantification. When the sample volume is 2mL,below samples will have a good linear relationship in the range of 5-80 ng·L-1 likenaphthalene, acenaphthene, fluorine, phenanthrene, anthraquinone, fluoranthene, pyrene, chrysene, benzo(a)anthracene, benzo(b)fluoranthene, benzo(k)fluoranthene, Benzo (a) pyrene, dibenzo (a, h) anthracene, benzo (g, h, i) pyrene, indole (1,2,3-cd) pyrene.And alsothiswater sample recovery ratehasexceeded 80%. This method can be used to detect 15 kinds of polycyclic aromatic hydrocarbon pollutants in water.
Key words:    polycyclic aromatic hydrocarbons    on-line solid phase extraction    ultra performance liquid chromatography   
收稿日期:
基金项目: 北京城市副中心重大地质问题-海绵城市地下水环境详细调查(2017-F-93)资助.
孙颖,Tel:(010)51560262,E-mail:277458218@qq.com
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参考文献:
[1] HJ 478-2009,水质多环芳烃的测定液液萃取和固相萃取高效液相色谱[S].北京:中国环境科学出版社,2009 HJ 478-2009,Water quality-Determination of polycyclic aromatic hydrocarbons-Liquid-liquid extraction and solid-phase extraction followed by high performance liquid chromatographic method[S].Beijing:China Environmental Press,2009(in Chinese).