Environmental Chemistry

ISSN 0254-6108

CN 11-1844/X

Vol. 38 No. 5
May  2019
Article Contents

Citation:

Determination of seleno amino acids in earthworms by high performance liquid chromatography-electrospray ionization-mass spectrometry

  • Received Date: 2019-01-14
    Fund Project:

    Supported by the National Natural Science Foundation of China (41671483).

  • A high performance liquid chromatography-electrospray ionization-mass spectrometry (HPLC-ESI-MS) method was developed for the determination of the seleno amino acids in earthworms. Selenomethione (SeMet) and methylselenocysteine (MeSeCyS) were extracted from earthworms by oscillating and ultrasound assisted enzymatic hydrolysis. These two seleno amino acids were isolated and determined by HPLC-ESI-MS using external standard method. Then the positive ion and multiple reaction monitoring (MRM) was applied and operated. The results indicated that both SeMet and MeSeCys had a good calibration curve in a range of 4-600 ng·mL-1, with R2 > 0.9999. The method detection limits of SeMet and MeSeCys were 0.49 μg·g-1 and 0.67 μg·g-1, respectively. The recoveries of standard addition were 81.1%-112.4%, with RSD values less than 3.1%. This detection method was preferred over others as it had eliminated the need for more cumbersome procedures, such as pre-column derivatization and increased the opposite direction gas.
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Determination of seleno amino acids in earthworms by high performance liquid chromatography-electrospray ionization-mass spectrometry

  • 1. College of Chemrtry & Chemical Engineering, Shanxi University, Taiyuan, 030006, China;
  • 2. School of Biotechnology and Health Sciences, Wuyi University, Jiangmen, 529020, China;
  • 3. State Key Laboratory of Urban and Regional Ecology, Research Centre for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing, 100085, China;
  • 4. College of Environmental Science and Resources, Shanxi University, Taiyuan, 030006, China
Fund Project:  Supported by the National Natural Science Foundation of China (41671483).

Abstract: A high performance liquid chromatography-electrospray ionization-mass spectrometry (HPLC-ESI-MS) method was developed for the determination of the seleno amino acids in earthworms. Selenomethione (SeMet) and methylselenocysteine (MeSeCyS) were extracted from earthworms by oscillating and ultrasound assisted enzymatic hydrolysis. These two seleno amino acids were isolated and determined by HPLC-ESI-MS using external standard method. Then the positive ion and multiple reaction monitoring (MRM) was applied and operated. The results indicated that both SeMet and MeSeCys had a good calibration curve in a range of 4-600 ng·mL-1, with R2 > 0.9999. The method detection limits of SeMet and MeSeCys were 0.49 μg·g-1 and 0.67 μg·g-1, respectively. The recoveries of standard addition were 81.1%-112.4%, with RSD values less than 3.1%. This detection method was preferred over others as it had eliminated the need for more cumbersome procedures, such as pre-column derivatization and increased the opposite direction gas.

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