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温度和底物浓度对混合菌群木糖厌氧生物产氢的影响

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张丹丹, 邱春生, 孙力平, 谢春雨, 胡鑫. 温度和底物浓度对混合菌群木糖厌氧生物产氢的影响[J]. 环境工程学报, 2015, 9(11): 5155-5160. doi: 10.12030/j.cjee.20151104
引用本文: 张丹丹, 邱春生, 孙力平, 谢春雨, 胡鑫. 温度和底物浓度对混合菌群木糖厌氧生物产氢的影响[J]. 环境工程学报, 2015, 9(11): 5155-5160. doi: 10.12030/j.cjee.20151104
shu
Zhang Dandan, Qiu Chunsheng, Sun Liping, Xie Chunyu, Hu Xin. Effects of temperature and substrate concentration on biological hydrogen production from xylose by mixed culture[J]. Chinese Journal of Environmental Engineering, 2015, 9(11): 5155-5160. doi: 10.12030/j.cjee.20151104
Citation: Zhang Dandan, Qiu Chunsheng, Sun Liping, Xie Chunyu, Hu Xin. Effects of temperature and substrate concentration on biological hydrogen production from xylose by mixed culture[J]. Chinese Journal of Environmental Engineering, 2015, 9(11): 5155-5160. doi: 10.12030/j.cjee.20151104
shu

温度和底物浓度对混合菌群木糖厌氧生物产氢的影响

  • 1.

    天津城建大学环境与市政工程学院, 天津 300384

  • 2.

    天津市水质科学与技术重点实验室, 天津 300384

  • 3.

    国网陕西省电力公司信息通讯公司, 西安 710004

  • 基金项目:

    国家自然科学基金资助项目(21206106)

    国家"水体污染控制与治理"科技重大专项(2012ZX07308-002)

  • 中图分类号: X172

Effects of temperature and substrate concentration on biological hydrogen production from xylose by mixed culture

  • 1.

    School of Municipal and Environmental Engineering, Harbin Institute of Technology, Harbin 150090, China

  • 2.

    State Key Laboratory of Urban Water Resource and Environment, Harbin 150090, China

  • 3.

    Telecommunication Company of Shanxi Electric Power Corporation, Xi'an 710004, China

  • Fund Project:

  • 摘要: 为了获得混合菌群利用木糖进行厌氧发酵产氢的最佳条件,通过批次实验,对中温(35℃)和高温(55℃)下混合菌群利用不同浓度木糖(10~50 g/L)厌氧发酵产氢系统进行了研究。结果表明,35℃下系统累积产氢量和最大氢气产率在底物浓度30 g/L时获得,乙醇和乙酸为主要产氢副产物,但继续提高底物浓度会造成系统VFAs的积累与pH下降,不利于木糖代谢产氢;而55℃下累积产氢量和氢气产率随底物浓度升高持续增长,乙醇为系统主要产氢副产物,VFAs累积量较少。高温下,虽然最大氢气产率和底物木糖降解量比35℃下的低,但有利于获得较为稳定的氢气产量,产氢系统在高底物浓度下也可保持较高的木糖降解率和较为稳定的pH,有利于木糖代谢产氢。
    Abstract: To obtain optimal conditions for anaerobic fermentation hydrogen from xylose by mixed culture, bio-hydrogen production from different concentrations of xylose(10-50 g/L) at mesophilic(35℃) and thermophilic temperatures(55℃) by mixed culture fermentation was investigated in batch operation. The results showed that the maximum cumulative hydrogen volume and hydrogen yield were obtained with substrate concentration of 30 g/L in the system at 35℃, and hydrogen production by-products of the system mainly was ethanol, followed by acetate, however, the increase of substrate concentration resulted in accumulation of VFAs and decrease of pH, which was not conducive to hydrogen production by xylose metabolism. However, with the increase of substrate concentration, the cumulative hydrogen volume and hydrogen yield increased at 55℃, by-products of the system was mainly ethanol with less accumulation of VFAs. Although the maximum hydrogen yield at 55℃ was lower than it at 35℃, it was conducive to obtain stable hydrogen output, hydrogen production system could maintain higher degradation efficiency of xylose and stable pH, which was conducive to hydrogen production at thermophilic temperature.
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  • 收稿日期:  2014-08-30
  • 刊出日期:  2015-11-18
张丹丹, 邱春生, 孙力平, 谢春雨, 胡鑫. 温度和底物浓度对混合菌群木糖厌氧生物产氢的影响[J]. 环境工程学报, 2015, 9(11): 5155-5160. doi: 10.12030/j.cjee.20151104
引用本文: 张丹丹, 邱春生, 孙力平, 谢春雨, 胡鑫. 温度和底物浓度对混合菌群木糖厌氧生物产氢的影响[J]. 环境工程学报, 2015, 9(11): 5155-5160. doi: 10.12030/j.cjee.20151104
shu
Zhang Dandan, Qiu Chunsheng, Sun Liping, Xie Chunyu, Hu Xin. Effects of temperature and substrate concentration on biological hydrogen production from xylose by mixed culture[J]. Chinese Journal of Environmental Engineering, 2015, 9(11): 5155-5160. doi: 10.12030/j.cjee.20151104
Citation: Zhang Dandan, Qiu Chunsheng, Sun Liping, Xie Chunyu, Hu Xin. Effects of temperature and substrate concentration on biological hydrogen production from xylose by mixed culture[J]. Chinese Journal of Environmental Engineering, 2015, 9(11): 5155-5160. doi: 10.12030/j.cjee.20151104
shu

温度和底物浓度对混合菌群木糖厌氧生物产氢的影响

  • 1.  天津城建大学环境与市政工程学院, 天津 300384
  • 2.  天津市水质科学与技术重点实验室, 天津 300384
  • 3.  国网陕西省电力公司信息通讯公司, 西安 710004
  • 收稿日期:  2014-08-30
基金项目:

国家自然科学基金资助项目(21206106)

国家"水体污染控制与治理"科技重大专项(2012ZX07308-002)

摘要: 为了获得混合菌群利用木糖进行厌氧发酵产氢的最佳条件,通过批次实验,对中温(35℃)和高温(55℃)下混合菌群利用不同浓度木糖(10~50 g/L)厌氧发酵产氢系统进行了研究。结果表明,35℃下系统累积产氢量和最大氢气产率在底物浓度30 g/L时获得,乙醇和乙酸为主要产氢副产物,但继续提高底物浓度会造成系统VFAs的积累与pH下降,不利于木糖代谢产氢;而55℃下累积产氢量和氢气产率随底物浓度升高持续增长,乙醇为系统主要产氢副产物,VFAs累积量较少。高温下,虽然最大氢气产率和底物木糖降解量比35℃下的低,但有利于获得较为稳定的氢气产量,产氢系统在高底物浓度下也可保持较高的木糖降解率和较为稳定的pH,有利于木糖代谢产氢。

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