稀土元素La(Ⅲ)对铜绿微囊藻生长和生理特性的影响
Effects of La(Ⅲ) on Growth and Physiological Characteristics of Microcystis aeruginosa
-
摘要: 为了解在营养元素氮、磷限制环境下稀土元素镧（La）对贫营养水体中铜绿微囊藻的影响及作用机制。在实验室模拟培养下，研究稀土元素镧对铜绿微囊藻（Microcystis aeruginosa）生长和生理特性的影响，测定缺素胁迫和正常情况不同La3+浓度对藻的生长量、叶绿素a、可溶性糖、可溶性蛋白、抗氧化酶活性（过氧化氢酶（CAT）、过氧化物酶（POD）以及丙二醛（MDA）含量的影响。结果表明，在缺磷胁迫下，低浓度La3+（0.10~0.20 mg·L-1）能刺激铜绿微囊藻的生长，增强光合作用效率，提高抗氧化酶活性；随着处理浓度的提高和处理时间的延长，藻类生长受到抑制，表现为光合色素、可溶性蛋白含量明显下降，可溶性糖含量增加，膜质过氧化程度加重。与正常情况的培养相比，在缺磷胁迫下，铜绿微囊藻对La3+的耐受力明显降低；而在缺氮情况下，La3+不能维持微囊藻的正常生长代谢。因此，一定浓度范围的稀土元素镧对缺磷及正常情况下的铜绿微囊藻生长有促进作用，是引起贫营养水体中铜绿微囊藻大量繁殖的诱因之一。Abstract: In order to understand the effect and mechanism of rare earth element lanthanum (La) on Microcystes aeruginosa in oligotrophic water under the limit environment of nutrient elements nitrogen and phosphorus. Under simulated cultivation in the laboratory, the effects of lanthanum on the growth and physiological characteristics of Microcystis aeruginosa were studied. The effects of different La3+ concentrations on the growth of algae, the contents of chlorophyll a, soluble sugar and soluble protein, the activity of antioxidant enzymes (catalase (CAT) and peroxidase (POD)), and the content of malondialdehyde (MDA), under nutrient deficiency stress and normal conditions were measured. The results showed that under phosphorus deficiency stress, low concentration of La3+ (0.10~0.20 mg·L-1) could stimulate the growth of Microcystis aeruginosa, enhance the photosynthetic efficiency and the activity of antioxidant enzymes. With the increase of treatment concentration and the extension of treatment time, the growth of algae was inhibited, the contents of photosynthetic pigment and soluble protein were obviously decreased, the content of soluble sugar was increased, and the degree of membrane peroxidation was aggravated. Compared with the normal culture, the tolerance of Microcystis aeruginosa to La3+ was significantly reduced under phosphorus deficiency stress. Microcystis aeruginosa could not maintain the normal growth and metabolism under the condition of nitrogen deficiency with La3+. Therefore, lanthanum in a certain concentration range can promote the growth of Microcystis aeruginosa in phosphorus deficiency and normal conditions, and is one of the inducements for the proliferation of Microcystis aeruginosa in oligotrophic water.
-
Key words:
- phosphorus /
- nitrogen /
- rare earth element /
- lanthanum /
- Microcystis aeruginosa /
- physiological characteristic
-
-
周云龙, 于明. 水华的发生、危害和防治[J]. 生物学通报, 2004, 39(6):11-14 袁侃, 毛献忠, 陶益, 等. UV-C辐照抑制铜绿微囊藻生长的动态实验研究[J]. 环境科学, 2010, 31(2):310-317 Yuan K, Mao X Z, Tao Y, et al. Dynamic experiment on controlling of Microcystis aeruginosa by UV-C irradiation[J]. Environmental Science, 2010, 31(2):310-317(in Chinese)
许可. 不同氮磷浓度对普通小球藻生长及光合作用的影响研究[D]. 西安:西安建筑科技大学, 2018:33-35 Xu K. Research on the growth and photosynthesis of Chlorella vulgaris under different nitrogen and phosphorus concentrations[D]. Xi'an:Xi'an University of Architecture and Technology, 2018:33 -35(in Chinese)
王应军, 伍钧, 金航标, 等. 稀土元素钕对铜绿微囊藻生长和生理特性的影响[J]. 农业环境科学学报, 2010, 29(7):1261-1267 Wang Y J, Wu J, Jin H B, et al. Effects of neodymium on the growth and physiological characteristics of Microcystis aeruginosa[J]. Journal of Agro-Environment Science, 2010, 29(7):1261-1267(in Chinese)
魏静, 林莉, 潘雄, 等. 不同环境胁迫因子对藻类分子生物学特性的影响研究进展[J]. 长江科学院院报, 2020, 37(4):14-24 Wei J, Lin L, Pan X, et al. Research progress about the effects of different environmental stress factors on algae in molecular biology[J]. Journal of Yangtze River Scientific Research Institute, 2020, 37(4):14-24(in Chinese)
Zhang L J, Yang T W, Gao Y S, et al. Effect of lanthanum ions (La3+) on ferritin-regulated antioxidant process under PEG stress[J]. Biological Trace Element Research, 2006, 113(2):193-208 詹鸿峰, 王华生, 潘禹, 等. 离子型稀土矿矿山废水检测与分析[J]. 中国稀土学报, 2020, 38(4):550-556 Zhan H F, Wang H S, Pan Y, et al. Measurement and analysis of ion-type rare earth mine wastewater[J]. Journal of the Chinese Society of Rare Earths, 2020, 38(4):550-556(in Chinese)
宋雪梅. 外源稀土对凤眼莲生长及水体富营养化影响研究[D]. 成都:四川师范大学, 2014:55 Song X M. The influence of rare earth on the growth of Eichhornia crassipes and the eutrophication[D]. Chengdu:Sichuan Normal University, 2014:55(in Chinese) 张杰, 黄永杰, 刘雪云. 镧对镉胁迫下水稻幼苗生长及生理特性的影响[J]. 生态环境, 2007, 16(3):835-841 Zhang J, Huang Y J, Liu X Y. Effects of La on growth and some physiological characteristics of rice seedlings under Cd stress[J]. Ecology and Environment, 2007, 16(3):835-841(in Chinese)
Xu C M, Zhao B, Wang X D, et al. Lanthanum relieves salinity-induced oxidative stress in Saussurea involucrate[J]. Biologia Plantarum, 2007, 51(3):567-570 黄晓华, 周青. 镧对水培菜豆和玉米幼苗镉胁迫的缓解作用[J]. 中国稀土学报, 2005, 23(2):245-249 Huang X H, Zhou Q. Protective effects of lanthanum on bean and corn under cadmium stress[J]. Journal of the Chinese Rare Earth Society, 2005, 23(2):245-249(in Chinese)
杜金戈, 王应军, 武阳, 等. 钇(Y3+)对缺氮、缺磷胁迫下铜绿微囊藻生长和生理特性及藻毒素释放的影响[J]. 农业环境科学学报, 2017, 36(8):1500-1507 Du J G, Wang Y J, Wu Y, et al. Effects of yttrium on growth and physiological characteristics and microcystin release of Microcystis aeruginosa under nitrogen and phosphorus deficiency[J]. Journal of Agro-Environment Science, 2017, 36(8):1500-1507(in Chinese)
张信连, 杨维东, 刘洁生, 等. 稀土元素生物效应中的hormesis现象[J]. 生物技术, 2004, 14(6):82-84 吕旭阳, 张雯, 杨阳, 等. 分光光度法测定小球藻数量的方法研究[J]. 安徽农业科学, 2009, 37(23):11104-11105 Lv X Y, Zhang W, Yang Y, et al. Methodological research on measuring Chlorella quantity by spectrophotometry[J]. Journal of Anhui Agricultural Sciences, 2009, 37(23):11104-11105(in Chinese)
陈建勋, 王晓峰. 植物生理学实验指导[M]. 广州:华南理工大学出版社, 2002:89-93 周永欣, 章宗涉. 水生生物毒性试验方法[M]. 北京:中国农业出版社, 1989:54-58 Deng Y, Wang W, Yu P F, et al. Comparison of taurine, GABA, Glu, and Asp as scavengers of malondialdehyde in vitro and in vivo[J]. Nanoscale Research Letters, 2013, 8(1):190 李合生. 植物生理生化实验原理和技术[M]. 北京:高等教育出版社, 2000:267-268 Li H X, Xiao Y, Cao L L, et al. Cerebroside C increases tolerance to chilling injury and alters lipid composition in wheat roots[J]. PLoS One, 2013, 8(9):e73380 Liu L, Liu Y, Cui J, et al. Oxidative stress induces gastric submucosal arteriolar dysfunction in the elderly[J]. World Journal of Gastroenterology, 2013, 19(48):9439-9446 张志良. 植物生理学实验指导[M]. 2版. 北京:高等教育出版社, 1990:160-162 岳冬梅, 李洁, 肖琳. 营养盐恢复对氮磷饥饿铜绿微囊藻生长的影响[J]. 环境科学, 2016, 37(11):4220-4227 Yue D M, Li J, Xiao L. Nutrients recovery on the growth of nitrogen and phosphorus starved Microcystis aeruginosa[J]. Environmental Science, 2016, 37(11):4220-4227(in Chinese)
崔宜淳. 稀土元素-镧、钕对两种淡水浮游藻类增殖的影响[D]. 北京:首都师范大学, 2007:34-38 Cui Y C. Effects of La/Nd on the cell multiplication of two phytoplanktons in fresh water[D]. Beijing:Capital Normal University, 2007:34 -38(in Chinese)
吕赟, 王应军, 冷雪, 等. 稀土铈对水华鱼腥藻生理特性及藻毒素释放的影响[J]. 农业环境科学学报, 2012, 31(9):1677-1683 Lv Y, Wang Y J, Leng X, et al. Effects of cerium on physiological characteristics and microcystins release of Anabaena flos-aquae[J]. Journal of Agro-Environment Science, 2012, 31(9):1677-1683(in Chinese)
杨维东, 刘洁生, 王艇, 等. Ce(NO3)3对大鼠内脏组织和大脑一氧化氮及合成酶的影响[J]. 稀土, 2000, 21(2):46-48 Yang W D, Liu J S, Wang T, et al. Effect of Ce(NO3)3 on the induction of NO and NOS in rats[J]. Chinese Rare Earths, 2000, 21(2):46-48(in Chinese)
Hu H W, Deng Y Y, Fan Y F, et al. Effects of artificial sweeteners on metal bioconcentration and toxicity on a green algae Scenedesmus obliquus[J]. Chemosphere, 2016, 150:285-293 Chen W J, Gu Y H, Zhao G W, et al. Effects of rare earth ions on activity of RuBPcase in tobacco[J]. Plant Science, 2000, 152(2):145-151 邱昌恩, 毕永红, 胡征宇. Zn2+胁迫对绿球藻生长、生理特性及细胞结构的影响[J]. 水生生物学报, 2007, 31(4):503-508 Qiu C E, Bi Y H, Hu Z Y. The effects of Zn2+ stress on the growth, physiological characteristics and cell structure of Chlorococcum sp.[J]. Acta Hydrobiologica Sinica, 2007, 31(4):503-508(in Chinese)
Sánchez F J, Manzanares M, de Andres E F, et al. Turgor maintenance, osmotic adjustment and soluble sugar and proline accumulation in 49 pea cultivars in response to water stress[J]. Field Crops Research, 1998, 59(3):225-235 王琼, 苏智先, 张素兰, 等. 慈竹构件和分株水平的可溶性糖含量研究[J]. 应用生态学报, 2004, 15(11):1994-1998 Wang Q, Su Z X, Zhang S L, et al. Soluble sugar content of clonal plant Neosinocalamus affinis at module and ramet levels[J]. Chinese Journal of Applied Ecology, 2004, 15(11):1994-1998(in Chinese)
丁海东, 万延慧, 齐乃敏, 等. 镉和锌对番茄幼苗抗氧化酶活性的影响[J]. 仲恺农业技术学院学报, 2004, 17(2):37-41 , 46 Ding H D, Wan Y H, Qi N M, et al. Effects of cadmium and zinc on activities of antioxidant enzymes in tomato seedlings[J]. Journal of Zhongkai Agrotechnical College, 2004, 17(2):37-41, 46(in Chinese)
计汪栋, 施国新, 杨海燕, 等. 铜胁迫对竹叶眼子菜叶片生理指标和超微结构的影响[J]. 应用生态学报, 2007, 18(12):2727-2732 Ji W D, Shi G X, Yang H Y, et al. Effects of Cu2+ stress on leaf physiological indice and ultrastructure of Potamogeton malaianus[J]. Chinese Journal of Applied Ecology, 2007, 18(12):2727-2732(in Chinese)
马成仓, 李清芳. Ca2+对汞毒害下的小麦种子萌发代谢的影响[J]. 农业环境保护, 2000, 19(3):173-175 Ma C C, Li Q F. Effect of Ca2+ on germination metabolism of wheat seed under mercury poisoning condition[J]. Agro-Environmental Protection, 2000, 19(3):173-175(in Chinese)
杨居荣, 贺建群, 蒋婉茹. Cd污染对植物生理生化的影响[J]. 农业环境保护, 1995, 14(5):193-197 Yang J R, He J Q, Jiang W R. Effect of Cd pollution on the physiology and biochemistry of plant[J]. Agro-Environmental Protection, 1995, 14(5):193-197(in Chinese)
杨燕生, 刘德, 龙白娟, 等. 镧对小麦幼苗素质、蛋白质及钙调素水平的影响[J]. 稀土, 1997, 18(2):63-65 王应军, 陈燕, 金航标, 等. 稀土Y3+对铜绿微囊藻生长和生理特性的影响[J]. 安徽农业科学, 2011, 39(4):2248-2251 Wang Y J, Chen Y, Jin H B, et al. Effects of yttrium (Y3+)on physiological and biochemical characteristics of Microcystis aeruginosa[J]. Journal of Anhui Agricultural Sciences, 2011, 39(4):2248-2251(in Chinese)
胡勤海, 管丽莉, 叶兆杰. 稀土元素对小球藻生长的影响[J]. 环境科学, 1996, 17(2):37-38 Hu Q H, Guan L L, Ye Z J. Effects of rare-earth elements on growth and reproduction of Chlorella pyrenoides[J]. Chinese Journal of Enviromental Science, 1996, 17(2):37-38(in Chinese)
袁婷, 樊驰, 王正银, 等. 钾肥对镉污染土壤中白菜生长与镉含量及保护酶活性的影响[J]. 安全与环境学报, 2016, 16(3):383-390 Yuan T, Fan C, Wang Z Y, et al. Effect of application of potassium fertilizers on the cabbage growth in Cd polluted soil and its protective enzyme activity in the cabbage content[J]. Journal of Safety and Environment, 2016, 16(3):383-390(in Chinese)
李合生. 现代植物生理学[M]. 北京:高等教育出版社, 2002:106-118 Pinto E, Sigaud-Kutner T C S, Leitao M A S, et al. Heavy metal-induced oxidative stress in algae[J]. Journal of Phycology, 2003, 39(6):1008-1018 刘璐, 闫浩, 夏文彤, 等. 镉对铜绿微囊藻和斜生栅藻的毒性效应[J]. 中国环境科学, 2014, 34(2):478-484 Liu L, Yan H, Xia W T, et al. Toxic effect of cadmium on Microcysis aeruginosa and Scenedesmus obliquus[J]. China Environmental Science, 2014, 34(2):478-484(in Chinese)
杨舒贻, 陈晓阳, 惠文凯, 等. 逆境胁迫下植物抗氧化酶系统响应研究进展[J]. 福建农林大学学报:自然科学版, 2016, 45(5):481-489 Yang S Y, Chen X Y, Hui W K, et al. Progress in responses of antioxidant enzyme systems in plant to environmental stresses[J]. Journal of Fujian Agriculture and Forestry University:Natural Science Edition, 2016, 45(5):481-489(in Chinese)
王应军, 金航标, 陈燕, 等. 稀土Y3+对铜绿微囊藻生长及其抗氧化酶活性的影响[J]. 四川农业大学学报, 2011, 29(1):108-113 Wang Y J, Jin H B, Chen Y, et al. Effects of yttrium(Y3+) on growth and antioxidant enzyme of Microcystis aeruginosa[J]. Journal of Sichuan Agricultural University, 2011, 29(1):108-113(in Chinese)
张春静, 王湧, 金鹏康. 重稀土元素对小球藻生长状态的影响[J]. 安全与环境学报, 2017, 17(6):2445-2451 Zhang C J, Wang Y, Jin P K. Influence of heavy rare earth elements on the growing status of Chlorella pyrenoidosa[J]. Journal of Safety and Environment, 2017, 17(6):2445-2451(in Chinese)
李天翠, 熊雄, 毕永红, 等. 激光对铜绿微囊藻的抑制效果及机理研究[J]. 环境科学与技术, 2015, 38(12):72-76 Li T C, Xiong X, Bi Y H, et al. Laser inhibition effects on Microcystis aeruginosa and mechanisms[J]. Environmental Science & Technology, 2015, 38(12):72-76(in Chinese)
Hejl A M, Koster K L. Juglone disrupts root plasma membrane H+-ATPase activity and impairs water uptake, root respiration, and growth in soybean (Glycine max) and corn (Zea mays)[J]. Journal of Chemical Ecology, 2004, 30(2):453-471 唐萍, 吴国荣, 陆长梅, 等. 凤眼莲根系分泌物对栅藻结构及代谢的影响[J]. 环境科学学报, 2000, 20(3):355-359 Tang P, Wu G R, Lu C M, et al. Effects of the excretion from root system of Eichhornia crassipes on the cell structure and metabolism of Scenedesmus arcuatus[J]. Acta Scientiae Circumstantiae, 2000, 20(3):355-359(in Chinese)
陈少裕. 膜脂过氧化对植物细胞的伤害[J]. 植物生理学通讯, 1991, 27(2):84-90 Chen S Y. Injury of membrane lipid peroxidation to plant cell[J]. Plant Physiology Communications, 1991, 27(2):84-90(in Chinese)
-
点击查看大图
计量
- 文章访问数: 2552
- HTML全文浏览数: 2552
- PDF下载数: 62
- 施引文献: 0
下载:
