聚苯乙烯纳米塑料暴露对小鼠肠道免疫的影响

贾田江; 陈怡; 张俊波; 李奡; 黄沛力

doi:10.7524/AJE.1673-5897.20210915002

生态毒理学报

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贾田江, 陈怡, 张俊波, 李奡, 黄沛力. 聚苯乙烯纳米塑料暴露对小鼠肠道免疫的影响[J]. 生态毒理学报, 2022, 17(3): 277-283. doi: 10.7524/AJE.1673-5897.20210915002

引用本文:

贾田江, 陈怡, 张俊波, 李奡, 黄沛力. 聚苯乙烯纳米塑料暴露对小鼠肠道免疫的影响[J]. 生态毒理学报, 2022, 17(3): 277-283. doi: 10.7524/AJE.1673-5897.20210915002

Jia Tianjiang, Chen Yi, Zhang Junbo, Li Ao, Huang Peili. Effects of Polystyrene Nanoplastics on Intestinal Immunity in Mice[J]. Asian journal of ecotoxicology, 2022, 17(3): 277-283. doi: 10.7524/AJE.1673-5897.20210915002

Citation:

Jia Tianjiang, Chen Yi, Zhang Junbo, Li Ao, Huang Peili. Effects of Polystyrene Nanoplastics on Intestinal Immunity in Mice[J]. Asian journal of ecotoxicology, 2022, 17(3): 277-283. doi: 10.7524/AJE.1673-5897.20210915002

聚苯乙烯纳米塑料暴露对小鼠肠道免疫的影响

1. 首都医科大学公共卫生学院, 北京 100069;
2. 环境毒理学北京市重点实验室, 北京 100069;
3. 首都医科大学基础医学实验教学中心医学机能学教学实验室, 北京 100069

作者简介: 贾田江(1996—),女,硕士研究生,研究方向为纳米毒理学,E-mail:13934601538@163.com

通讯作者: 陈怡, E-mail: cherly8611@sina.com ; 黄沛力, E-mail: huangpl@ccmu.edu.cn

基金项目:
国家自然科学基金面上项目（82173569）
中图分类号: X171.5

Effects of Polystyrene Nanoplastics on Intestinal Immunity in Mice

1. School of Public Health, Capital Medical University, Beijing 100069, China;
2. Beijing Key Laboratory of Environmental Toxicology, Beijing 100069, China;
3. Medical Function Teaching Laboratory of Basic Medical Experimental Teaching Center, Capital Medical University, Beijing 100069, China

Corresponding authors: Chen Yi, cherly8611@sina.com ; Huang Peili, huangpl@ccmu.edu.cn

Fund Project:

摘要: 纳米塑料（NPs）的生态风险评估和潜在毒性效应研究正在逐步成为全球公共卫生领域关注的热点。本研究探究聚苯乙烯纳米塑料（PS NPs）对小鼠肠道免疫的影响，将40只雌性BALB/c小鼠随机分为4组，每组10只，分别为对照组、0.5 mg·kg^-1（以体质量计）染毒组、5 mg·kg^-1染毒组和50 mg·kg^-1染毒组。采用灌胃的方式进行染毒，连续灌胃PS NPs悬液7 d，对照组小鼠灌胃超纯水。通过观察小鼠生长状况、免疫相关血生化指标、小肠组织形态和结肠黏液分泌情况的变化，探究了PS NPs暴露对小鼠肠道免疫的影响。结果表明，与对照组相比，随着染毒剂量的增加，小鼠体质量增长缓慢，补体C3含量下降（高剂量组，P<0.05），小肠结构受损，结肠黏液分泌减少。提示，PS NPs可能通过损伤肠道免疫屏障进而干扰机体免疫稳态，且其影响作用与PS NPs的含量呈正相关。研究结果将为NPs污染的潜在毒性效应评价提供基础数据。
- 聚苯乙烯纳米塑料 /
- 小鼠 /
- 肠道免疫
Abstract: The ecological risk assessment and potential toxic effects of nanoplastics (NPs) are becoming the focus of global public health. The present study aims to explore the effects of polystyrene nanoplastics (PS NPs) on intestinal immunity in mice. In the animal experiment 40 female BALB/c mice were randomly divided into four groups with ten mice in each group, which are control group, 0.5 mg·kg^-1 dose group, 5 mg·kg^-1 dose group and 50 mg·kg^-1 dose group, respectively. PS NPs suspension was intragastrically administered for 7 d, and ultrapure water treated group was the control. The effects of PS NPs exposure on intestinal immunity in mice were investigated by observing the growth status, immune-related blood biochemical indexes, small intestinal tissue morphology and colonic mucus secretion. The results showed that the body weight of mice increased slowly, complement C3 content decreased (high dose group, P<0.05), small intestine structure was damaged, and colon mucus secretion decreased in a dose-dependent manner, compared with the control group. These results suggested that PS NPs might disturb immune homeostasis by damaging the intestinal immune barrier, and its effects are positively correlated with the dose of PS NPs. This study will provide significant information for evaluating the potential toxic effects of NPs pollution.
- polystyrene nanoplastics /
- mice /
- intestinal immunity

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贾田江, 陈怡, 张俊波, 李奡, 黄沛力. 聚苯乙烯纳米塑料暴露对小鼠肠道免疫的影响[J]. 生态毒理学报, 2022, 17(3): 277-283. doi: 10.7524/AJE.1673-5897.20210915002

引用本文:

贾田江, 陈怡, 张俊波, 李奡, 黄沛力. 聚苯乙烯纳米塑料暴露对小鼠肠道免疫的影响[J]. 生态毒理学报, 2022, 17(3): 277-283. doi: 10.7524/AJE.1673-5897.20210915002

Citation:

聚苯乙烯纳米塑料暴露对小鼠肠道免疫的影响

通讯作者: 陈怡, E-mail: cherly8611@sina.com ; 黄沛力, E-mail: huangpl@ccmu.edu.cn

作者简介: 贾田江(1996—),女,硕士研究生,研究方向为纳米毒理学,E-mail:13934601538@163.com
1. 首都医科大学公共卫生学院, 北京 100069;

2. 环境毒理学北京市重点实验室, 北京 100069;

3. 首都医科大学基础医学实验教学中心医学机能学教学实验室, 北京 100069

收稿日期: 2021-09-15

基金项目:

国家自然科学基金面上项目（82173569）

关键词:

摘要: 纳米塑料（NPs）的生态风险评估和潜在毒性效应研究正在逐步成为全球公共卫生领域关注的热点。本研究探究聚苯乙烯纳米塑料（PS NPs）对小鼠肠道免疫的影响，将40只雌性BALB/c小鼠随机分为4组，每组10只，分别为对照组、0.5 mg·kg^-1（以体质量计）染毒组、5 mg·kg^-1染毒组和50 mg·kg^-1染毒组。采用灌胃的方式进行染毒，连续灌胃PS NPs悬液7 d，对照组小鼠灌胃超纯水。通过观察小鼠生长状况、免疫相关血生化指标、小肠组织形态和结肠黏液分泌情况的变化，探究了PS NPs暴露对小鼠肠道免疫的影响。结果表明，与对照组相比，随着染毒剂量的增加，小鼠体质量增长缓慢，补体C3含量下降（高剂量组，P<0.05），小肠结构受损，结肠黏液分泌减少。提示，PS NPs可能通过损伤肠道免疫屏障进而干扰机体免疫稳态，且其影响作用与PS NPs的含量呈正相关。研究结果将为NPs污染的潜在毒性效应评价提供基础数据。

English Abstract

Effects of Polystyrene Nanoplastics on Intestinal Immunity in Mice

Corresponding author: Chen Yi, cherly8611@sina.com ;

Corresponding authors: Huang Peili, huangpl@ccmu.edu.cn

1. School of Public Health, Capital Medical University, Beijing 100069, China;
2. Beijing Key Laboratory of Environmental Toxicology, Beijing 100069, China;
3. Medical Function Teaching Laboratory of Basic Medical Experimental Teaching Center, Capital Medical University, Beijing 100069, China

Received Date: 2021-09-15

Fund Project:

Keywords:

Abstract: The ecological risk assessment and potential toxic effects of nanoplastics (NPs) are becoming the focus of global public health. The present study aims to explore the effects of polystyrene nanoplastics (PS NPs) on intestinal immunity in mice. In the animal experiment 40 female BALB/c mice were randomly divided into four groups with ten mice in each group, which are control group, 0.5 mg·kg^-1 dose group, 5 mg·kg^-1 dose group and 50 mg·kg^-1 dose group, respectively. PS NPs suspension was intragastrically administered for 7 d, and ultrapure water treated group was the control. The effects of PS NPs exposure on intestinal immunity in mice were investigated by observing the growth status, immune-related blood biochemical indexes, small intestinal tissue morphology and colonic mucus secretion. The results showed that the body weight of mice increased slowly, complement C3 content decreased (high dose group, P<0.05), small intestine structure was damaged, and colon mucus secretion decreased in a dose-dependent manner, compared with the control group. These results suggested that PS NPs might disturb immune homeostasis by damaging the intestinal immune barrier, and its effects are positively correlated with the dose of PS NPs. This study will provide significant information for evaluating the potential toxic effects of NPs pollution.