废水急性毒性测定及评价中的不确定性研究及其改进方法
Uncertainty Studies in Determination and Assessment of Acute Toxicity of Wastewater and Its Improvement Method
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摘要: 目前，我国针对废水处理及排放的环境影响评估体系逐渐向生物毒性综合指标完善，然而传统的评价方法可能存在错误的接受和错误的拒绝2种风险，根据“检测和校准实验室能力认可准则”(CNAS-CL01: 2018)的要求，实验室在进行合格判定时，应考虑相关的风险水平。为研究分析废水急性毒性评价中存在的风险，采用发光细菌法测试废水急性毒性，选取接近排放限值的制药废水样品，进行测量不确定度评定，并依据“产品几何量技术规范(GPS) 工件的测量检验和测量设备 第1部分：按规范检验一致性或不一致性的判定规则”(ISO 14253-1: 2017)有关合格判定的思路，对废水急性毒性评价中的合格区、不确定区和不合格区进行研究。结果表明，废水急性毒性评价中，合格区、不确定区及不合格区会随着标准曲线|r|值和测量精密度的变化而变化，随着|r|值的降低或精密度的降低，合格区会变小，不确定区会变大，这导致了无法通过设置“保护带”的方法降低误判风险，对废水急性毒性评价的准确性造成了极大的影响。为准确开展废水急性毒性的测试与评价，可采用双样本t检验，通过比较废水样品的相对发光度与0.07 mg·L-1氯化汞溶液的相对发光度来判断废水急性毒性是否合格，当废水样品的相对发光度≥0.07 mg·L-1氯化汞溶液的相对发光度时，废水急性毒性合格，否则不合格。本研究通过开展方法的不确定度研究，阐明了现行国标分析方法及废水急性毒性评价方法存在的局限性，并提出了改进方案，为废水急性毒性的科学评判提供了重要依据。Abstract: Comprehensive biotoxicity indicators have been considered into environmental impact assessment system of wastewater treatment and discharge in China. However, the traditional methods of biotoxicity assessment face two risks of incorrect acceptance and incorrect rejection. According to Accreditation Criteria for the Competence of Testing and Calibration Laboratories (CNAS-CL01: 2018), laboratories should consider the risks when making conformity assessment. This study investigated the uncertainty evaluation of the luminescent bacteria test for the acute toxicity of wastewater, involving its conformance, uncertainty, and non-conformance zones (Geometrical Product Specifications (GPS)—Inspection by Measurement of Workpieces and Measuring Equipment—Part 1: Decision Rules for Verifying Conformity or Nonconformity with Specifications (ISO 14253-1: 2017)), using the pharmaceutical wastewater sample close to the discharge limits. The results show that the conformance, uncertainty, and non-conformance zones varied depending on the r-value of the standard curve and the precision of the measurement. The conformance zone decreased and uncertainty zone increased with the |r|-value or the precision decreased, which made it impossible to reduce the risk of incorrectly decision by setting a “guard band”, and greatly affected the accuracy of the acute toxicity assessment. To solve this problem, a Welch's t-test was introduced to assess whether the acute toxicity of wastewater was conformance by comparing the relative luminosity of the wastewater with that of the 0.07 mg·L-1 HgCl2 solution. If the relative luminosity of the wastewater was higher than that of the 0.07 mg·L-1 HgCl2 solution, the acute toxicity of the wastewater was conformance, otherwise the acute toxicity of the wastewater was non-conformance. This study clarifies the limitations of the national standard method and the acute toxicity assessment by carrying out the uncertainty study, and proposes an improvement, which provide an important basis for the scientific assessment of the acute toxicity of wastewater.
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李鑫. 多元生物毒素联合毒性统计分析[D]. 北京: 北京林业大学, 2016: 2-5 Li X. Statistical analysis of joint toxicity of biotoxins[D]. Beijing: Beijing Forestry University, 2016: 2 -5(in Chinese)
Li X, Pi L, Nie M, et al. Joint effects of rotational extrusion and TiO2 on performance and antimicrobial properties of extruded polypropylene copolymer pipes[J]. Journal of Applied Polymer Science, 2015, 132(33): 51-61 Uribe R, Fuentes-García A. The effects of TV unhealthy food brand placement on children. Its separate and joint effect with advertising[J]. Appetite, 2015, 91: 165-172 孙成华, 刘康, 衡丽娜, 等. 水样发光细菌急性毒性测试的方式与表征[J]. 中国环境监测, 2014, 30(6): 123-128 Sun C H, Liu K, Heng L N, et al. Luminescent bacteria acute toxicity test of water and its characterization[J]. Environmental Monitoring in China, 2014, 30(6): 123-128(in Chinese)
朱金国, 刘红霞. 发光细菌检测四环素族抗生素体系的建立[J]. 中国食品卫生杂志, 2005, 17(2): 150-153 Zhu J G, Liu H X. System constructed to detect tetrayclines drug by luminescent bacteria[J]. Chinese Journal of Food Hygiene, 2005, 17(2): 150-153(in Chinese)
王亚群. 利用海洋发光细菌进行水产品中氯霉素残留检测的方法研究[D]. 青岛: 中国海洋大学, 2008: 10-12 Wang Y Q. Studies on the determination of chloramphenicol residues in the aquatic foods by luminescent bacteria[D]. Qingdao: Ocean University of China, 2008: 10 -12(in Chinese)
Ma X Y, Wang X C, Liu Y J. Study of the variation of ecotoxicity at different stages of domestic wastewater treatment using Vibrio-qinghaiensis sp.-Q67[J]. Journal of Hazardous Materials, 2011, 190(1-3): 100-105 高品, 于晓霏, 杨婧, 等. 工业废水综合毒性评价方法及其应用研究进展[J]. 工业水处理, 2023. 10.19965/j.cnki.iwt.2023-0334 中国合格评定国家认可委员会. CNAS-CL01: 2018检测和校准实验室能力认可准则[S/OL].[2023-11-19]. https://www.cnas.org.cn/rkgf/sysrk/jbzz/2019/02/895558.shtml International Organization for Standardization (ISO), International Electrotechnical Commission (IEC). ISO/IEC Guide 98-4: 2012, Uncertainty of measurement: Part 4: Role of measurement uncertainty in conformity assessment[S]. Geneva: ISO, 2012 International Organization for Standardization. Geometrical product specifications (GPS): Inspection by measurement of workpieces and measuring equipment: Part 1: Decision rules for verifying conformity or nonconformity with specifications: ISO 14253-1: 2017[S]. Geneva: International Organization for Standardization, 2017 中华人民共和国环境保护部. 发酵类制药工业水污染物排放标准: GB 21903—2008[S]. 北京: 中国环境科学出版社, 2008 中华人民共和国环境保护部. 化学合成类制药工业水污染物排放标准: GB 21904—2008[S]. 北京: 中国环境科学出版社, 2008 中华人民共和国环境保护部. 提取类制药工业水污染物排放标准: GB 21905—2008[S]. 北京: 中国环境科学出版社, 2008 中华人民共和国环境保护部. 中药类制药工业水污染物排放标准: GB 21906—2008[S]. 北京: 中国环境科学出版社, 2008 中华人民共和国环境保护部. 生物工程类制药工业水污染物排放标准: GB 21907—2008[S]. 北京: 中国环境科学出版社, 2008 中华人民共和国环境保护部. 混装制剂类制药工业水污染物排放标准: GB 21908—2008[S]. 北京: 中国环境科学出版社, 2008 国家环境保护局, 国家技术监督局. 水质急性毒性的测定发光细菌法: GB/T 15441—1995[S]. 北京: 中国标准出版社, 1995 倪育才. 实用测量不确定度评定[M]. 5版. 北京: 中国质检出版社, 2006: 244 李真, 苗晶晶, 潘鲁青. 6种重金属的发光菌毒性效应及其海洋生物物种敏感度分析[J]. 海洋环境科学, 2015, 34(2): 176-183 Li Z, Miao J J, Pan L Q. Toxicity effect of heavy metals on Photobacterium phosphoreum and the species sensitivity comparison with other marine organisms[J]. Marine Environmental Science, 2015, 34(2): 176-183(in Chinese)
杜丽娜, 薛金玲, 余若祯, 等. 对《水质急性毒性的测定发光细菌法》的改进和探讨[J]. 中国环境监测, 2013, 29(6): 123-127 Du L N, Xue J L, Yu R Z, et al. Improvement and exploration for the water quality-determination of the acute toxicity-luminescent bacteria test[J]. Environmental Monitoring in China, 2013, 29(6): 123-127(in Chinese)
岳舜琳, 潘海祥. 用发光细菌监测水质突发性污染的适用性[J]. 给水排水, 2008, 34(3): 28-31 Yue S L, Pan H X. Applicability of illuminescent bacteria examination for incident water pollution monitoring[J]. Water & Wastewater Engineering, 2008, 34(3): 28-31(in Chinese)
中华人民共和国环境保护部. 水质氨氮的测定纳氏试剂分光光度法: HJ 535—2009[S]. 北京: 中国环境科学出版社, 2010 国家环境保护总局. 污水综合排放标准: GB 8978—1996[S]. 北京: 中国标准出版社, 1998 -
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