硒暴露对斑马鱼(Danio rerio)组织元素含量及氧化胁迫的影响研究
Effects of Tissue Element Content and Oxidative Stress after Zebrafish (Danio rerio) Exposed to Selenium
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摘要: 以斑马鱼为受试材料，研究了Se(Ⅳ)暴露对斑马鱼组织的元素含量及氧化胁迫的影响。试验分4组，分别为对照组，5 μg·L-1、50 μg·L-1和500 μg·L-1暴露组，处理21 d后，对斑马鱼各组织(脑、鳃、肝、肠、肌肉)中的Se、Al、Cr、Cu、Mg和Zn的含量进行测定，同时测定各组织中超氧化物歧化酶(SOD)活性、谷胱甘肽-S-转移酶(GST)活性、还原型谷胱甘肽(GSH)含量和脂质过氧化产物(MDA)含量。结果显示，与对照组相比，硒处理组斑马鱼各组织呈现不同程度的硒累积，肝组织中硒累积量最高，且元素Cu和Zn显著增加。肝组织中SOD和GSH水平呈现先升高后降低的趋势，500 μg·L-1处理组肝组织中GST活性显著降低56.5%，而肝和鳃组织中MDA分别显著升高3.1倍和1.6倍。研究结果表明硒暴露破坏斑马鱼组织Cu、Zn元素平衡，引起脂质过氧化，诱导斑马鱼肝鳃组织发生氧化胁迫，试验为进一步从元素平衡和氧化胁迫角度研究硒暴露对水生生物的毒性效应奠定了基础。Abstract: This study aimed to explore the effects of selenium (Se) exposure on the element content and oxidative stress in zebrafish (Danio rerio) tissues. The treatments were divided into four groups, i.e., control group, 5 μg·L-1-exposed group, 50 μg·L-1-exposed group, 500 μg·L-1-exposed group. The levels of Se, Al, Cr, Cu, Mg and Zn, superoxide dismutase (SOD), reduced glutathione (GSH), glutathione S-transferase (GST) and malonyldialdehyde (MDA) were determined in different tissues (brain, gill, liver, intestine, and muscle) of zebrafish after exposure to Se for 21 days. The results showed that different extents of Se accumulation occurred in fish tissues in the Se-exposed groups compared to the control treatment. Specifically, Se accumulation was the highest and Zn and Cu increased remarkably in the liver. GSH and SOD in liver tissues firstly increased and then decreased significantly. The activity of GST was significantly decreased by 56.5% in 500 μg·L-1-exposed liver tissues. However, MDA was significantly increased by 3.1-fold in liver tissues and by 1.6-fold in gill tissues, respectively. These results demonstrated that Se exposure destroyed the balance of Cu and Zn in zebrafish tissues, caused lipid peroxidation, and induced oxidative stress in zebrafish liver and gill tissues. Our findings lay a foundation for further study in the toxic effects of selenium exposure on aquatic organisms from the perspective of element balance and oxidative stress.
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Key words:
- selenium accumulation /
- zebrafish /
- element content /
- oxidative stress
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