新型酶联免疫测定废气中二噁英类物质

周志广, 赵斌, 许鹏军, 任玥, 李楠, 齐丽, 郑森, 赵虎, 范爽, 张烃, 刘爱民, 黄业茹, 西井重名. 新型酶联免疫测定废气中二噁英类物质[J]. 环境化学, 2013, 32(7): 1358-1364. doi: 10.7524/j.issn.0254-6108.2013.07.033
引用本文: 周志广, 赵斌, 许鹏军, 任玥, 李楠, 齐丽, 郑森, 赵虎, 范爽, 张烃, 刘爱民, 黄业茹, 西井重名. 新型酶联免疫测定废气中二噁英类物质[J]. 环境化学, 2013, 32(7): 1358-1364. doi: 10.7524/j.issn.0254-6108.2013.07.033
ZHOU Zhiguang, ZHAO Bin, XU Pengjun, REN Yue, LI Nan, QI Li, ZHENG Sen, ZHAO Hu, FAN Shuang, ZHANG Ting, LIU Aimin, HUANG Yeru, Shigeaki Nishii. Determination of PCDD/Fs in exhaust gas by new ELISA[J]. Environmental Chemistry, 2013, 32(7): 1358-1364. doi: 10.7524/j.issn.0254-6108.2013.07.033
Citation: ZHOU Zhiguang, ZHAO Bin, XU Pengjun, REN Yue, LI Nan, QI Li, ZHENG Sen, ZHAO Hu, FAN Shuang, ZHANG Ting, LIU Aimin, HUANG Yeru, Shigeaki Nishii. Determination of PCDD/Fs in exhaust gas by new ELISA[J]. Environmental Chemistry, 2013, 32(7): 1358-1364. doi: 10.7524/j.issn.0254-6108.2013.07.033

新型酶联免疫测定废气中二噁英类物质

  • 基金项目:

    国家环保公益性科研专项"城市环境二噁英监测技术规范与快速监测技术研究"(200909095)

    国家重点基础研究计划(973)项目(2009CB421602)资助.

Determination of PCDD/Fs in exhaust gas by new ELISA

  • Fund Project:
  • 摘要: 与传统酶联免疫分析方法检测2,3,7,8-TCDD以及采用毒性较大的2,3,7,8-TCDD做标准曲线不同,本文构建了一种新的单克隆抗体用于检测PeCDFs以及HxCDFs,并且采用新型毒性较低的2,4,5-TCP-(Gly)2制作标准曲线,同时优化了此种反应系统的反应条件,并采用此系统分析了来自中国的24个废气样品,计算出了换算方程,将结果与HRGC-HRMS进行了比较,呈现了良好的相关性,两者的相关系数R2为0.944,在中国可以应用此方法来进行二噁英类筛查性分析.
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  • [1] Lorber M, Eschenroeder A, Robinson. Testing the USA EPA’s ISCST-Version 3 model on dioxins: A comparison of predicted and observed air and soil concentrations [J]. Atmos Eviron, 2000, 34(23): 3995-4010
    [2] Nadal M, Agramunt, M C, Schuhmacher M, et al. PCDD/PCDF congener profiles in soil and herbage samples collected in the vicinity of a municipal waste incinerator before and after pronounced reductions of PCDD/PCDF emissions from the facility[J]. Chemosphere, 2002, 49: 153-159
    [3] Xu P, Tao B, Li N, et al. Levels, profiles and source identification of PCDD/Fs in farmland soils of Guiyu, China[J]. Chemosphere, 2013, 91 (6): 824-831
    [4] Li C, Zheng M H, Zhang B, et al. Levels of PCDD/Fs, Dl-PCBs and HCB in air, soils and sediments from a city with multiple thermal-related industries in China[J]. Bull Environ Contam Toxicol, 2012, 89 (3): 542-546
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    [7] Hicscherora K, Kannan K, Nakata H, et al. Polychlorinated dibenzo-p-dioxin and dibenzofuran concentration profiles in sediment and flood-plain soils of the Tittabawassee River, Michigan[J]. Environ Sci Technol, 2003, 37: 468-474
    [8] Pan J, Yang Y, Taniyasu S, et al. Comparison of historical record of PCDD/Fs, dioxin-like PCBs, and PCNs in sediment cores from Jiaozhou Bay and coastal Yellow Sea: Implication of different sources[J]. Bull Environ Contam Toxicol, 2012, 89 (6): 1240-1246
    [9] Lohmann R, Jones K E. Dioxins and furans in air and deposition: A review of levels, behavior and processes[J]. Sci Total Environ, 1998, 219: 53-81
    [10] QuassU, Fermann M, Broker G. The European dioxin air emission inventory project—final results[J]. Chemosphere, 2004, 54: 1319-1327
    [11] Chen T, Li X D, Yan J H, et al. Distribution of polychlorinated dibenzo-p-dioxins and dibenzofurans in ambient air of different regions in China[J]. Atmospheric Environment, 2011, 45: 6567-6575
    [12] Lin C W, TSAI C H, Guo P, et al. Characterization of polybrominated dibenzo-p-dioxins and dibenzofurans in different atmospheric Environments[J]. Environ Sci Technol, 2008, 42: 75-80
    [13] Huwe J K, Agric J. Dioxins in food:A modern agricultural perspective[J]. Food Chem, 2002, 50: 1739-1750
    [14] Sun S J, Kayama F, Zhao J H, et al. Longitudinal increases in PCDD/F and dl-PCB concentrations in human milk in northern China[J]. Chemosphere, 2011, 85 (3): 448-453
    [15] Song Y, Wu N, Han J, et al. Levels of PCDD/Fs and DL-PCBs in selected foods and estimated dietary intake for the local residents of Luqiao and Yuhang in Zhejiang, China[J]. Chemosphere, 2011, 85 (3): 329-334
    [16] Watanabe M X, Kunisue T, Ueda N, et al. Toxicokinetics of dioxins and other organochlorine compounds in Japanese people: Association with hepatic CYP1A2 expression levels[J]. Environ Int, 2013, 53: 53-61
    [17] Shen H, Ding G, Wu Y, et al. Polychlorinated dibenzo-p-dioxins/furans (PCDD/Fs), polychlorinated biphenyls (PCBs), and polybrominated diphenyl ethers (PBDEs) in breast milk from Zhejiang, China[J]. Environ Int, 2012, 42: 84-97
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    [20] Hiroki F, Shigeaki N, Kohei N, et al. Development of a quick and efficient analysis method of dioxins using ELISA[J]. Organohalogen Compound, 2003, 60: 311-314
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  • 收稿日期:  2013-02-27
周志广, 赵斌, 许鹏军, 任玥, 李楠, 齐丽, 郑森, 赵虎, 范爽, 张烃, 刘爱民, 黄业茹, 西井重名. 新型酶联免疫测定废气中二噁英类物质[J]. 环境化学, 2013, 32(7): 1358-1364. doi: 10.7524/j.issn.0254-6108.2013.07.033
引用本文: 周志广, 赵斌, 许鹏军, 任玥, 李楠, 齐丽, 郑森, 赵虎, 范爽, 张烃, 刘爱民, 黄业茹, 西井重名. 新型酶联免疫测定废气中二噁英类物质[J]. 环境化学, 2013, 32(7): 1358-1364. doi: 10.7524/j.issn.0254-6108.2013.07.033
ZHOU Zhiguang, ZHAO Bin, XU Pengjun, REN Yue, LI Nan, QI Li, ZHENG Sen, ZHAO Hu, FAN Shuang, ZHANG Ting, LIU Aimin, HUANG Yeru, Shigeaki Nishii. Determination of PCDD/Fs in exhaust gas by new ELISA[J]. Environmental Chemistry, 2013, 32(7): 1358-1364. doi: 10.7524/j.issn.0254-6108.2013.07.033
Citation: ZHOU Zhiguang, ZHAO Bin, XU Pengjun, REN Yue, LI Nan, QI Li, ZHENG Sen, ZHAO Hu, FAN Shuang, ZHANG Ting, LIU Aimin, HUANG Yeru, Shigeaki Nishii. Determination of PCDD/Fs in exhaust gas by new ELISA[J]. Environmental Chemistry, 2013, 32(7): 1358-1364. doi: 10.7524/j.issn.0254-6108.2013.07.033

新型酶联免疫测定废气中二噁英类物质

  • 1.  国家环境分析测试中心,国家环境保护二噁英污染控制重点实验室, 北京, 100029;
  • 2.  中国科学院生态环境研究中心,环境化学与生态毒理学国家重点实验室, 北京, 100085;
  • 3.  东洋纺敦贺生物研究所,日本敦贺, 914-0047
基金项目:

国家环保公益性科研专项"城市环境二噁英监测技术规范与快速监测技术研究"(200909095)

国家重点基础研究计划(973)项目(2009CB421602)资助.

摘要: 与传统酶联免疫分析方法检测2,3,7,8-TCDD以及采用毒性较大的2,3,7,8-TCDD做标准曲线不同,本文构建了一种新的单克隆抗体用于检测PeCDFs以及HxCDFs,并且采用新型毒性较低的2,4,5-TCP-(Gly)2制作标准曲线,同时优化了此种反应系统的反应条件,并采用此系统分析了来自中国的24个废气样品,计算出了换算方程,将结果与HRGC-HRMS进行了比较,呈现了良好的相关性,两者的相关系数R2为0.944,在中国可以应用此方法来进行二噁英类筛查性分析.

English Abstract

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