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基于表面增强拉曼研究好氧菌株对砷的原位还原过程

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刘颖, 张建锋, 杜晶晶. 基于表面增强拉曼研究好氧菌株对砷的原位还原过程[J]. 环境化学, 2015, 34(7): 1275-1280. doi: 10.7524/j.issn.0254-6108.2015.07.2014111808
引用本文: 刘颖, 张建锋, 杜晶晶. 基于表面增强拉曼研究好氧菌株对砷的原位还原过程[J]. 环境化学, 2015, 34(7): 1275-1280. doi: 10.7524/j.issn.0254-6108.2015.07.2014111808
shu
LIU Ying, ZHANG Jianfeng, DU Jingjing. In-situ Surface enhanced Raman scattering characterization of arsenate reduction mediated by Pantoea sp.IMH[J]. Environmental Chemistry, 2015, 34(7): 1275-1280. doi: 10.7524/j.issn.0254-6108.2015.07.2014111808
Citation: LIU Ying, ZHANG Jianfeng, DU Jingjing. In-situ Surface enhanced Raman scattering characterization of arsenate reduction mediated by Pantoea sp.IMH[J]. Environmental Chemistry, 2015, 34(7): 1275-1280. doi: 10.7524/j.issn.0254-6108.2015.07.2014111808
shu

基于表面增强拉曼研究好氧菌株对砷的原位还原过程

  • 1.

    西安建筑科技大学, 西安, 710055;

  • 2.

    中国科学院生态环境研究中心, 北京, 100085

  • 基金项目:

    国家重大科学仪器设备开发专项(2011YQ0301241002)资助.

In-situ Surface enhanced Raman scattering characterization of arsenate reduction mediated by Pantoea sp.IMH

  • 1.

    Xi'an University of Architecture and Technology, Xi'an, 710055, China;

  • 2.

    Research Center for Eco-Environmental Sciences, Chinese Academy of Science, Beijing, 100085, China

  • Fund Project:

  • 摘要: 从内蒙古高砷地区土壤中分离得到好氧菌株Pantoea sp. IMH,通过场发射扫描电镜与透射电镜表征该菌株形貌为短棒状、周生鞭毛.最小生长抑制浓度测定结果表明菌株IMH对As(Ⅴ)和As(Ⅲ)均有一定抗性.砷还原动力学过程研究表明菌株IMH具有高效砷还原能力.利用表面增强拉曼散射(Surface enhanced Raman scattering, SERS)作为表征手段,采用自主计的流动检测装置,检测到了菌株IMH的30 min内的砷还原过程.通过对菌株砷还原机理的探究认为,arsH、arsC、arsB和arsR等4种基因片段在调控砷还原过程中起到了主要作用.
    Abstract: An aerobic As(Ⅴ)-reducing bacteria-Pantoea sp. IMH was isolated from arsenic (As)-contaminated soil in Inner Mongolia, China. Its morphology was characterized by field emission scanning electron microscopy and transmission electron microscopy. The bacteria is rod-shaped with flagella around. Minimum Inhibitory Concentration test indicated that bacteria IMH had certain resistance towards As(Ⅴ) and As(Ⅲ). The highly efficient arsenate reduction capacity of bacteria IMH was verified by the kinetic studies. Real-time detection of arsenate bio-reduction processsin 30 min was achieved by a self-disigned flow-cell using Surface enhanced Ramen scattering (SERS). Based on the research of the mechanism,we believe that arsH、arsC、arsB and arsR genes played a major role in arsenate reduction process.
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出版历程
  • 收稿日期:  2014-11-18
  • 刊出日期:  2015-07-15
刘颖, 张建锋, 杜晶晶. 基于表面增强拉曼研究好氧菌株对砷的原位还原过程[J]. 环境化学, 2015, 34(7): 1275-1280. doi: 10.7524/j.issn.0254-6108.2015.07.2014111808
引用本文: 刘颖, 张建锋, 杜晶晶. 基于表面增强拉曼研究好氧菌株对砷的原位还原过程[J]. 环境化学, 2015, 34(7): 1275-1280. doi: 10.7524/j.issn.0254-6108.2015.07.2014111808
shu
LIU Ying, ZHANG Jianfeng, DU Jingjing. In-situ Surface enhanced Raman scattering characterization of arsenate reduction mediated by Pantoea sp.IMH[J]. Environmental Chemistry, 2015, 34(7): 1275-1280. doi: 10.7524/j.issn.0254-6108.2015.07.2014111808
Citation: LIU Ying, ZHANG Jianfeng, DU Jingjing. In-situ Surface enhanced Raman scattering characterization of arsenate reduction mediated by Pantoea sp.IMH[J]. Environmental Chemistry, 2015, 34(7): 1275-1280. doi: 10.7524/j.issn.0254-6108.2015.07.2014111808
shu

基于表面增强拉曼研究好氧菌株对砷的原位还原过程

  • 1.  西安建筑科技大学, 西安, 710055;
  • 2.  中国科学院生态环境研究中心, 北京, 100085
  • 收稿日期:  2014-11-18
基金项目:

国家重大科学仪器设备开发专项(2011YQ0301241002)资助.

摘要: 从内蒙古高砷地区土壤中分离得到好氧菌株Pantoea sp. IMH,通过场发射扫描电镜与透射电镜表征该菌株形貌为短棒状、周生鞭毛.最小生长抑制浓度测定结果表明菌株IMH对As(Ⅴ)和As(Ⅲ)均有一定抗性.砷还原动力学过程研究表明菌株IMH具有高效砷还原能力.利用表面增强拉曼散射(Surface enhanced Raman scattering, SERS)作为表征手段,采用自主计的流动检测装置,检测到了菌株IMH的30 min内的砷还原过程.通过对菌株砷还原机理的探究认为,arsH、arsC、arsB和arsR等4种基因片段在调控砷还原过程中起到了主要作用.

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