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pH对间歇进水序批式生物反应(SBR)工艺活性污泥沉降性能和微生物结构的影响

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璩绍雷, 孙宝盛, 赵双红, 臧向荣, 李志静, 魏佳虹. pH对间歇进水序批式生物反应(SBR)工艺活性污泥沉降性能和微生物结构的影响[J]. 环境化学, 2016, 35(3): 508-515. doi: 10.7524/j.issn.0254-6108.2016.03.2015073101
引用本文: 璩绍雷, 孙宝盛, 赵双红, 臧向荣, 李志静, 魏佳虹. pH对间歇进水序批式生物反应(SBR)工艺活性污泥沉降性能和微生物结构的影响[J]. 环境化学, 2016, 35(3): 508-515. doi: 10.7524/j.issn.0254-6108.2016.03.2015073101
shu
QU Shaolei, SUN Baosheng, ZHAO Shuanghong, ZANG Xiangrong, LI Zhijing, WEI Jiahong. pH activated sludge sedimentation performance and the structure of the microbe of SBR technology[J]. Environmental Chemistry, 2016, 35(3): 508-515. doi: 10.7524/j.issn.0254-6108.2016.03.2015073101
Citation: QU Shaolei, SUN Baosheng, ZHAO Shuanghong, ZANG Xiangrong, LI Zhijing, WEI Jiahong. pH activated sludge sedimentation performance and the structure of the microbe of SBR technology[J]. Environmental Chemistry, 2016, 35(3): 508-515. doi: 10.7524/j.issn.0254-6108.2016.03.2015073101
shu

pH对间歇进水序批式生物反应(SBR)工艺活性污泥沉降性能和微生物结构的影响

  • 1.

    天津大学环境科学与工程学院, 天津, 300072

  • 基金项目:

    天津市应用基础研究计划项目(07JCZDJC02100)资助.

pH activated sludge sedimentation performance and the structure of the microbe of SBR technology

  • 1.

    School of Environmental Science and Engineering, Tianjin University, Tianjin, 300072, China

  • Fund Project: Supported by Applied Basic Research Project of Tianjin(07JCZDJC02100).

  • 摘要: 本文主要研究了不同pH值对SBR工艺污泥膨胀和微生物结构的影响,试验进水pH值为5.0-10.0.实验结果表明,在试验初期,污泥容积指数(SVI)值无明显变化.但随着时间的增长,在pH值为5.0和6.0时,开始出现污泥膨胀.在不同pH下提取总细菌的DNA组进行PCR扩增,分析总细菌的Shannon多样性指数,对微生物群落的部分优势总细菌进行克隆测序和系统发育树分析.结果表明,在不同pH条件下微生物结构以及数量有所区别,在酸性条件下以丝状菌为主,易发生污泥膨胀;而在中性和偏碱性条件下以菌胶团为主,污泥活性较为稳定.
    Abstract: This paper mainly studied the influence of pH on SBR sludge bulking and microbial structure with the pH values of the inflow water from 5.0 to 10.0. The results showed that the did not change initially of Sludge Volume Index (SVI) value. However, the sludge bulking was seen over time when pH values became 5.0 and 6.0. At various pH values, the total bacterial DNA groups were extracted for PCR amplification, and then relevant analyses were given about Shannon diversity index, cloned sequencing and phylogenetic tree of partial dominant total bacteria in the microbial communities. This study demonstrated that the structures and quantities of microorganisms were different at different pH values:in acidic conditions filamentous bacteria became dominant and they were more accessible to sludge bulking in neutral and alkaline condition while zoogloea were the dominant ones and the activity of sludge was relatively stable.
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  • 收稿日期:  2015-07-31
  • 刊出日期:  2016-03-15
璩绍雷, 孙宝盛, 赵双红, 臧向荣, 李志静, 魏佳虹. pH对间歇进水序批式生物反应(SBR)工艺活性污泥沉降性能和微生物结构的影响[J]. 环境化学, 2016, 35(3): 508-515. doi: 10.7524/j.issn.0254-6108.2016.03.2015073101
引用本文: 璩绍雷, 孙宝盛, 赵双红, 臧向荣, 李志静, 魏佳虹. pH对间歇进水序批式生物反应(SBR)工艺活性污泥沉降性能和微生物结构的影响[J]. 环境化学, 2016, 35(3): 508-515. doi: 10.7524/j.issn.0254-6108.2016.03.2015073101
shu
QU Shaolei, SUN Baosheng, ZHAO Shuanghong, ZANG Xiangrong, LI Zhijing, WEI Jiahong. pH activated sludge sedimentation performance and the structure of the microbe of SBR technology[J]. Environmental Chemistry, 2016, 35(3): 508-515. doi: 10.7524/j.issn.0254-6108.2016.03.2015073101
Citation: QU Shaolei, SUN Baosheng, ZHAO Shuanghong, ZANG Xiangrong, LI Zhijing, WEI Jiahong. pH activated sludge sedimentation performance and the structure of the microbe of SBR technology[J]. Environmental Chemistry, 2016, 35(3): 508-515. doi: 10.7524/j.issn.0254-6108.2016.03.2015073101
shu

pH对间歇进水序批式生物反应(SBR)工艺活性污泥沉降性能和微生物结构的影响

  • 1. 天津大学环境科学与工程学院, 天津, 300072
  • 收稿日期:  2015-07-31
基金项目:

天津市应用基础研究计划项目(07JCZDJC02100)资助.

摘要: 本文主要研究了不同pH值对SBR工艺污泥膨胀和微生物结构的影响,试验进水pH值为5.0-10.0.实验结果表明,在试验初期,污泥容积指数(SVI)值无明显变化.但随着时间的增长,在pH值为5.0和6.0时,开始出现污泥膨胀.在不同pH下提取总细菌的DNA组进行PCR扩增,分析总细菌的Shannon多样性指数,对微生物群落的部分优势总细菌进行克隆测序和系统发育树分析.结果表明,在不同pH条件下微生物结构以及数量有所区别,在酸性条件下以丝状菌为主,易发生污泥膨胀;而在中性和偏碱性条件下以菌胶团为主,污泥活性较为稳定.

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