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臭氧灭活水中枯草芽孢杆菌的动力学

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刘枫, 昌盛, 陈忠林. 臭氧灭活水中枯草芽孢杆菌的动力学[J]. 环境化学, 2016, 35(5): 858-864. doi: 10.7524/j.issn.0254-6108.2016.05.2015122101
引用本文: 刘枫, 昌盛, 陈忠林. 臭氧灭活水中枯草芽孢杆菌的动力学[J]. 环境化学, 2016, 35(5): 858-864. doi: 10.7524/j.issn.0254-6108.2016.05.2015122101
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LIU Feng, CHANG Sheng, CHEN Zhonglin. Inactivation kinetics of Bacillus subtilis spores with ozone[J]. Environmental Chemistry, 2016, 35(5): 858-864. doi: 10.7524/j.issn.0254-6108.2016.05.2015122101
Citation: LIU Feng, CHANG Sheng, CHEN Zhonglin. Inactivation kinetics of Bacillus subtilis spores with ozone[J]. Environmental Chemistry, 2016, 35(5): 858-864. doi: 10.7524/j.issn.0254-6108.2016.05.2015122101
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臭氧灭活水中枯草芽孢杆菌的动力学

  • 1.

    哈尔滨工业大学, 城市水资源与水环境国家重点实验室, 哈尔滨, 150090;

  • 2.

    中国环境科学研究院, 国家环境保护饮用水水源地保护重点实验室, 北京, 100012

  • 基金项目:

    国家自然科学基金(51508539),国家水体污染控制与治理重大专项(2014ZX07405-001,2014ZX07415-002)资助.

Inactivation kinetics of Bacillus subtilis spores with ozone

  • 1.

    State Key Laboratory of Urban Water Resource and Environment, School of Municipal & Environmental Engineering, Harbin Institute of Technology, Harbin, 150090, China;

  • 2.

    State Environmental Protection Key Laboratory of Drinking Water Source Protection, Chinese Research Academy of Environmental Sciences, Beijing, 100012, China

  • Fund Project: Supported by the National Natural Science Foundation (51508539), National Major Projects of Water Pollution Control and Treatment (2014ZX07405-001, 2014ZX07415-002).

  • 摘要: 以枯草芽孢杆菌(ATCC6633)的孢子作为难灭活微生物的代表,研究了消毒剂浓度和反应时间的乘积值(CT值)、pH值、温度对臭氧灭活水中芽孢效果的影响,并探讨了相关灭活反应的动力学特征.结果表明,臭氧灭活芽孢的过程可分为延滞期和灭活期,其灭活反应符合Chick-Watson延迟反应动力学模型.在半连续流反应模式下,当臭氧浓度在0.42-4.00 mg·L-1,反应时间0-20 min,pH值6-8,温度1-30℃范围内时,臭氧对芽孢的灭活效果与臭氧的CT值显著相关,与单独的臭氧浓度无关,CT值越高,所能达到的灭活率也越高.同时,温度对反应速率常数k影响较大,即随着温度的升高,灭活反应的延滞期CTlag显著减小,反应速率常数k增大,臭氧对芽孢的灭活能力增强;而反应速率常数k在各pH值下基本不变,pH值对芽孢的灭活影响甚微.
    Abstract: In general, spores of Bacillus subtilis (ATCC6633) would be used as potential model for the resistant microorganisms. In this study, the inactivation kinetics of spores in drinking water by ozone was investigated, and the factors such as ozone such as the numerical value of the product of the concentration of ozone and the reaction time (CT) values, pH, and temperature which could influence the inactivation process were evaluated. Results showed that the inactivation process of spores with ozone was characterized by a lag phase followed by a logarithmic inactivation phase, and the delayed Chick-Watson model could well describe the inactivation process. In this study, the disinfection of Bacillus subtilis spores was performed in a semi-batch reactor under the conditions with the ozone concentration, reaction time, pH, and temperature ranged in 0.42-4.00 mg·L-1, 0-20 min, 6-8, and 1-30℃, respectively. It showed that the inactivation is independent of ozone dose and is obvious relative to the ozone CT values. A higher inactivation level of spores would be achieved at higher CT values. In addition, the results indicated that the temperature could affect the kinetic rate constant of disinfection, and the lag phase decreased and the rate constant increased with the increasing of temperature. However, the kinetic rate constant kept nearly stead under different pH, which indicated that the pH had insignificant effect on spores inactivation.
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  • [1] SHANNON M A, BOHN P W, ELIMELECH M E, et al Science and technology for water purification in the coming decades[J]. Nature, 2008, 452(20): 301-310
    [2] YEON J J, BYUNG S O, JOON W K. Synergistic effect of sequential or combined use of ozone and UV radiation for the disinfection of Bacillus subtilis spores[J]. Water Research, 2008, 42(6-7): 1613-1621
    [3] STLOW B, MCGINNIS K A, RAGKOUSI K, et al. Effects of major spore-specific DNA binding proteins on Bacillus subtilis sporulation and spore properties[J]. Bacteriology, 2000, 182(24): 6906-6912
    [4] ESSAM A M, GUI S P, CHOI I W, et al. Comparison of Fe(Ⅵ) (Fe) and ozone in inactivating Bacillus subtilis spores[J]. Chemosphere, 2011, 83(9): 1228-1233
    [5] ERICK R B, ROBERTO P, ANGEL E V L, et al. Bacillus subtilis spore inactivation in water using photo-assisted Fenton reaction[J]. Sustain Environmental Research, 2011, 21(5): 285-290
    [6] CHO M, GANDHI V, HWANG T M, et al. Investigating synergism during sequential inactivation of MS-2 phage and Bacillus subtilis spores with UV/H2O2 followed by free chlorine[J]. Water Research, 2011, 45(3): 1063-1070
    [7] CHO M, CHUNG H, YOON J. Effect of pH and importance of ozone initiated radical reactions in inactivating Bacillus subtilis spore[J]. Ozone Science Engineering, 2002, 24(2): 145-150
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  • 收稿日期:  2015-12-21
  • 刊出日期:  2016-05-15

臭氧灭活水中枯草芽孢杆菌的动力学

  • 1.  哈尔滨工业大学, 城市水资源与水环境国家重点实验室, 哈尔滨, 150090;
  • 2.  中国环境科学研究院, 国家环境保护饮用水水源地保护重点实验室, 北京, 100012
  • 收稿日期:  2015-12-21
基金项目:

国家自然科学基金(51508539),国家水体污染控制与治理重大专项(2014ZX07405-001,2014ZX07415-002)资助.

摘要: 以枯草芽孢杆菌(ATCC6633)的孢子作为难灭活微生物的代表,研究了消毒剂浓度和反应时间的乘积值(CT值)、pH值、温度对臭氧灭活水中芽孢效果的影响,并探讨了相关灭活反应的动力学特征.结果表明,臭氧灭活芽孢的过程可分为延滞期和灭活期,其灭活反应符合Chick-Watson延迟反应动力学模型.在半连续流反应模式下,当臭氧浓度在0.42-4.00 mg·L-1,反应时间0-20 min,pH值6-8,温度1-30℃范围内时,臭氧对芽孢的灭活效果与臭氧的CT值显著相关,与单独的臭氧浓度无关,CT值越高,所能达到的灭活率也越高.同时,温度对反应速率常数k影响较大,即随着温度的升高,灭活反应的延滞期CTlag显著减小,反应速率常数k增大,臭氧对芽孢的灭活能力增强;而反应速率常数k在各pH值下基本不变,pH值对芽孢的灭活影响甚微.

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