4-BDE胁迫对毛白杨组培苗不定根发生的影响
Effcets of 4-BDE on adventitious rooting of tissue culture seedlings of Populus tomentosa
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摘要: 为了解一溴代联苯醚（4-monobrominated diphenyl ether，4-BDE）的植物修复机制，本文研究了不同浓度4-BDE处理对毛白杨组培苗不定根的形态、分化状况、细胞组织学以及部分生理特性的影响.发现一定浓度的4-BDE处理对毛白杨组培苗不定根的分化表现出先抑制后促进的效应，说明4-BDE可能具有一定的植物生长素生物效应.在4-BDE的诱导处理下，组培苗不定根的颜色变深，300 mg·L-1时根呈黑褐色且受到明显的伸长抑制，但并未坏死.与对照苗相比，4-BDE诱导处理的毛白杨组培苗不定根的显微结构发生明显变化，表现为根皮层细胞内含物增多，皮层细胞排列变紧密.高浓度30 mg·L-1 4-BDE处理23 d的组培苗不定根丙二醛（MDA）含量显著高于处理47 d和58 d的（P-1 4-BDE处理的过氧化物酶（POD）活性显著高于对照组（P-1 4-BDE处理的组培苗不定根的MDA含量呈现先上升后下降的趋势，POD活性呈逐渐上升的趋势.以上结果初步显示，毛白杨组培苗不定根对4-BDE胁迫具有一定的适应能力.Abstract: In order to understand the mechanisms of phytoremediation of 4-BDE(4-monobrominated diphenyl ether), the effects of 4-BDE on adventitious roots differentiation, phytocytohistology and some physiological-biochemical characteristics on tissue culture plantlet of Populus tomentosa were investigated. The results showed that 4-BDE promoted the differentiation of adventitious roots after an initial inhibition in a certain concentration range, which indicated that 4-BDE might have a certain biological effect on plant growth hormone. The adventitious roots under the exposure of 4-BDE became dark in color in comparison with the control, and 300 mg·L-1 4-BDE had a remarkable restraining effect on the length of adventitious roots. Compared with the control, the microstructure of adventitious roots treated with 4-BDE changed significantly. The cellular contents within adventitious roots increased and the cortical cells became closely arranged. In addition, for 30 mg·L-1 4-BDE treatment, MDA (Malondialdehyde) content of adventitious roots on 23th day was significantly higher than the content on 47th day and 58th day (P-1 4-BDE treatment was higher than that of the control(P-1 4-BDE treatment, the MDA content first increased with treatment time and then decreased, while POD activity presented a gradually increasing trend. These results showed that the adventitious roots of tissue culture plantlet of P. tomentosa had a certain adaptability to 4-BDE stress.
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