高级搜索

水体中新烟碱类杀虫剂的固相萃取方法优化：响应曲面法

,  ,  , 

downloadPDF

上一篇

下一篇

张俊杰, 韦燕莉, 李慧珍, 游静. 水体中新烟碱类杀虫剂的固相萃取方法优化：响应曲面法[J]. 环境化学, 2017, 36(5): 1064-1071. doi: 10.7524/j.issn.0254-6108.2017.05.2016091202
引用本文: 张俊杰, 韦燕莉, 李慧珍, 游静. 水体中新烟碱类杀虫剂的固相萃取方法优化：响应曲面法[J]. 环境化学, 2017, 36(5): 1064-1071. doi: 10.7524/j.issn.0254-6108.2017.05.2016091202
shu
ZHANG Junjie, WEI Yanli, LI Huizhen, YOU Jing. Optimizing solid phase extraction method for neonicotinoids in water: Application of response surface methodology[J]. Environmental Chemistry, 2017, 36(5): 1064-1071. doi: 10.7524/j.issn.0254-6108.2017.05.2016091202
Citation: ZHANG Junjie, WEI Yanli, LI Huizhen, YOU Jing. Optimizing solid phase extraction method for neonicotinoids in water: Application of response surface methodology[J]. Environmental Chemistry, 2017, 36(5): 1064-1071. doi: 10.7524/j.issn.0254-6108.2017.05.2016091202
shu

水体中新烟碱类杀虫剂的固相萃取方法优化：响应曲面法

  • 1.

    暨南大学环境学院, 广东省环境污染与健康重点实验室和广州市环境暴露与健康重点实验室, 广州, 510632;

  • 2.

    中国科学院广州地球化学研究所, 有机地球化学国家重点实验室, 广州, 510640

  • 基金项目:

    中国博士后科学基金（2015M582431），国家自然科学基金（41503091，41473106） 和广东省自然科学基金（2015A030310219，2016A030312009）资助

Optimizing solid phase extraction method for neonicotinoids in water: Application of response surface methodology

  • 1.

    School of Environment, Guangzhou Key Laboratory of Environmental Exposure and Health and Guangdong Key Laboratory of Environmental Pollution and Health, Jinan University, Guangzhou, 510632, China;

  • 2.

    State Key Laboratory of Organic Geochemistry, Guangzhou Institute of Geochemistry, Chinese Academy of Sciences, Guangzhou, 510640, China

  • Fund Project: Supported by China Postdoctoral Science Foundation (2015M582431), the National Science Foundation of China (41503091, 41473106) and the Natural Science Foundation of Guangdong Province (2015A030310219, 2016A030312009)

  • 摘要: 建立了一种固相萃取（SPE）前处理，高效液相色谱/质谱联用分析（HPLC/MS）测定水体中痕量新烟碱类杀虫剂（啶虫脒、噻虫胺、呋虫胺、吡虫啉、噻虫啉和噻虫嗪）含量的方法.确立HPLC/MS分析方法，并采用响应曲面法中Box-Behnken实验设计优化了SPE的吸附剂类型和用量、洗脱液类型和用量，获得以100 mg HLB为吸附剂及10 mL甲醇为洗脱液的前处理方法.所建方法适用于分析水中较宽浓度范围（3个数量级：0.9—100 ng·mL-1）的新烟碱类杀虫剂，回收率范围为75.4%±0.98%—122%±1.7%.此外，目标物的方法检测限均低于3 ng·L-1，相对标准偏差范围为2.99%—7.92%，低于文献值，说明所建方法具有较好的灵敏度和精密度.最后，该方法成功用于分析野外采集的水样，验证了该方法分析环境水体中新烟碱类杀虫剂的适用性.
    Abstract: An method has been established for determining trace neonicotinoid insecticides (acetamiprid, clothianidin, dinotefuran, imidacloprid, thiacloprid and thiamethoxa) in water using high performance liquid chromatography/mass spectrometry (HPLC/MS) after sample preparation with solid phase extraction (SPE). After setting up HPLC-MS method, response surface methodology (Box-Behnken design) was applied to select the type and mass of sorbents and the type and volume of elution solution for SPE. The optimized SPE method used 100 mg HLB as the sorbent and 10 mL methanol as the elution solvent. The newly developed method was validated by analyzing target insecticides in water in a wide range of concentrations (up to three multitudes: 0.9 ng·L-1—100 ng·mL-1), and the recovery of analytes ranged from (75.4±0.98)% to (122±1.7)%. The method detection limits for all neonicotinoids were less than 3 ng·L-1 with the relative standard deviations from 2.99% to 7.92%. The values were lower than those in previous studies, indicating good sensitivity and precision of the established method. Eventually, the method was successfully applied to determine neonicotinoids in field water samples, validating the effectiveness of the newly developed method in the environment.
  • 加载中
  • [1] JESCHKE P, NAUEN R, SCHINDLER M,et al. Overview of the status and global strategy for neonicotinoids[J]. Journal of Agricultural and Food Chemistry, 2011, 59: 2897-2908.
    [2] GILLBURN AS, BUNNEFELD N, WILSON J M, et al. Are neonicotinoid insecticides driving declines of widespread butterflies?[J]. Peer J, 2015, 3: e1402.
    [3] RUNDLOF M, ANDERSSON G K, BOMMARCO R, et al. Seed coating with a neonicotinoid insecticide negatively affects wild bees[J]. Nature, 2015, 521: 77-80.
    [4] HALLMANN C A, FOPPEN R P, VAN TURNHOUT C A, et al. Declines in insectivorous birds are associated with high neonicotinoid concentrations[J]. Nature, 2014, 511: 341-343.
    [5] AGATZ A, ASHAUER R, BROWN C D. Imidacloprid perturbs feeding of Gammarus Pulex at environmentally relevant concentrations[J]. Environmental Toxicology and Chemistry, 2014, 33: 648-653.
    [6] ROESSINK I, MERGA L B, ZWEERS H J, et al. The neonicotinoid imidacloprid shows high chronic toxicity to mayfly nymphs[J]. Environmental Toxicology and Chemistry, 2013, 32: 1096-1100.
    [7] RIAZ M A, CHANDOR-PROUST A, DAUPHIN-VILLEMANT C, et al. Molecular mechanisms associated with increased tolerance to the neonicotinoid insecticide imidacloprid in the dengue vector Aedes Aegypti[J]. Aquatic Toxicology, 2013, 126: 326-337.
    [8] STARNER K, GOH K S. Detections of the neonicotinoid insecticide imidacloprid in surface waters of three agricultural regions of California, USA, 2010-2011[J]. Bulletin of Environmental Contamination and Toxicology, 2012, 88: 316-321.
    [9] MORRISSEY C A, MINEAU P, DEVRIES J H, et al. Neonicotinoid contamination of global surface waters and associated risk to aquatic invertebrates: A review[J]. Environment International, 2015, 74: 291-303.
    [10] MAIN A R, HEADLEY J V, PERU K M, et al. Widespread use and frequent detection of neonicotinoid insecticides in wetlands of Canada's Prairie Pothole Region[J]. PloS One, 2014, 9(3): e92821.
    [11] YOKOYAMA S, ITO M, NAGASAWA S, et al. Runoff and degradation of aerially applied dinotefuran in paddy fields and river[J]. Bulletin of Environmental Contamination and Toxicology, 2015, 94: 796-800.
    [12] FUENTES E, CID C, BAEZ M E. Determination of imidacloprid in water samples via photochemically induced fluorescence and second-order multivariate calibration[J]. Talanta, 2015, 134: 8-15.
    [13] SANCHEZ-BAYO F, HYNE R V. Detection and analysis of neonicotinoids in river waters-development of a passive sampler for three commonly used insecticides[J]. Chemosphere, 2014, 99: 143-151.
    [14] HLADIK M L, CALHOUN D L. Analysis of the herbicide diuron, three diuron degradates, and six neonicotinoid insecticides in water-method details and application to two Georgia streams[R]: U.S. Geological, 2012.
    [15] BEZERRA M A, SANTELI R E, OLIVEIRA E P, et al. Response surface methodology (RSM) as a tool for optimization in analytical chemistry[J]. Talanta, 2008, 76: 965-977.
    [16] YETILMEZSOY K, DEMIREL S, VANDERBEI R J. Response surface modeling of Pb(Ⅱ) removal from aqueous solution by Pistacia vera L.: Box-Behnken experimental design[J]. Journal of Hazardous Materials, 2009, 171: 551-562.
    [17] LI Y L, FANG Z X, YOU J. Application of Box-Behnken experimental design to optimize the extraction of insecticidal Cry1Ac from soil[J]. Journal of Agricultural and Food Chemistry, 2013, 61: 1464-1470.
    [18] YIN G, DANG Y. Optimization of extraction technology of the Lycium barbarum polysaccharides by Box-Behnken statistical design[J]. Carbohydrate Polymers, 2008, 74: 603-610.
    [19] BEZERRA M A, SANTELLI R E, OLIVEIRA E P, et al. Response surface methodology (RSM) as a tool for optimization in analytical chemistry[J]. Talanta, 2008, 76: 965-977.
    [20] JIN S, BLUEMLING B, MOL A P J. Information, trust and pesticide overuse: Interactions between retailers and cotton farmers in China[J]. NJAS-Wageningen Journal of Life Sciences, 2015, 72-73: 23-32.
    [21] 李菊颖,何健,吴文铸,等. 蜂蜜中9 种杀虫剂的UPLC-MS/MS 检测方法[J].环境化学, 2016, 35(9): 1921-1927.

    LI J Y, HE J, WU W Z, et al. UPLC-MS/MS detection of 9 insecticides in honey[J].Environmental Chemistry, 2016, 35(9):1921-1927(in Chinese).

    [22] SANCHEZ-BAYO F. The trouble with neonicotinoids[J]. Science, 2014, 346: 806-807.
  • 加载中
WeChat 点击查看大图
计量
  • 文章访问数:  914
  • HTML全文浏览数:  859
  • PDF下载数:  501
  • 施引文献:  0
出版历程
  • 收稿日期:  2016-09-21
  • 刊出日期:  2017-05-15
张俊杰, 韦燕莉, 李慧珍, 游静. 水体中新烟碱类杀虫剂的固相萃取方法优化：响应曲面法[J]. 环境化学, 2017, 36(5): 1064-1071. doi: 10.7524/j.issn.0254-6108.2017.05.2016091202
引用本文: 张俊杰, 韦燕莉, 李慧珍, 游静. 水体中新烟碱类杀虫剂的固相萃取方法优化：响应曲面法[J]. 环境化学, 2017, 36(5): 1064-1071. doi: 10.7524/j.issn.0254-6108.2017.05.2016091202
shu
ZHANG Junjie, WEI Yanli, LI Huizhen, YOU Jing. Optimizing solid phase extraction method for neonicotinoids in water: Application of response surface methodology[J]. Environmental Chemistry, 2017, 36(5): 1064-1071. doi: 10.7524/j.issn.0254-6108.2017.05.2016091202
Citation: ZHANG Junjie, WEI Yanli, LI Huizhen, YOU Jing. Optimizing solid phase extraction method for neonicotinoids in water: Application of response surface methodology[J]. Environmental Chemistry, 2017, 36(5): 1064-1071. doi: 10.7524/j.issn.0254-6108.2017.05.2016091202
shu

水体中新烟碱类杀虫剂的固相萃取方法优化：响应曲面法

  • 1.  暨南大学环境学院, 广东省环境污染与健康重点实验室和广州市环境暴露与健康重点实验室, 广州, 510632;
  • 2.  中国科学院广州地球化学研究所, 有机地球化学国家重点实验室, 广州, 510640
  • 收稿日期:  2016-09-21
基金项目:

中国博士后科学基金（2015M582431），国家自然科学基金（41503091，41473106） 和广东省自然科学基金（2015A030310219，2016A030312009）资助

摘要: 建立了一种固相萃取（SPE）前处理，高效液相色谱/质谱联用分析（HPLC/MS）测定水体中痕量新烟碱类杀虫剂（啶虫脒、噻虫胺、呋虫胺、吡虫啉、噻虫啉和噻虫嗪）含量的方法.确立HPLC/MS分析方法，并采用响应曲面法中Box-Behnken实验设计优化了SPE的吸附剂类型和用量、洗脱液类型和用量，获得以100 mg HLB为吸附剂及10 mL甲醇为洗脱液的前处理方法.所建方法适用于分析水中较宽浓度范围（3个数量级：0.9—100 ng·mL-1）的新烟碱类杀虫剂，回收率范围为75.4%±0.98%—122%±1.7%.此外，目标物的方法检测限均低于3 ng·L-1，相对标准偏差范围为2.99%—7.92%，低于文献值，说明所建方法具有较好的灵敏度和精密度.最后，该方法成功用于分析野外采集的水样，验证了该方法分析环境水体中新烟碱类杀虫剂的适用性.

English Abstract

    全文HTML

参考文献 (22)

返回顶部

目录

/

返回文章
返回

Copyright © 2019 中国科学院生态环境研究中心