污水处理过程中抗生素抗性基因的检测及其水平转移机制的研究进展
Detection and horizontal transfer of antibiotic resistance genes during wastewater treatment processes
-
摘要: 抗生素的滥用使得近年来环境中抗生素频繁检出,同时污水处理厂接纳不同来源的污水后也促进了抗性基因的水平转移.现阶段抗性基因和水平转移的定性、定量检测手段多样化,尤其是水平转移的机制也备受关注.本文介绍了抗生素抗性基因和水平转移的检测方法以及抗性基因水平转移的研究进展,展望了今后的发展方向,以期为抑制抗性基因的扩散提供技术参考.Abstract: Antibiotics are frequently detected in environment these days due to their abuse. Their horizontal genes transfer (HGT) in wastewater treatment plants is intensified because wastewater from different source is collected here (There are various). Qualitative and quantitative detection methods of antibiotic resistance genes(ARGs) and horizontal transfer at present, and the pathways of HGT have particularly received great attention. This review introduces the detection method of ARGs and HGT, summarizes the research progress on horizontal transfer of ARGs, then proposes the direction of future work aimed to provide technical reference for the elimination of dissemination of ARGs.
-
-
[1] DANTAS G, SOMMER M O. Context matters-the complex interplay between resistome genotypes and resistance phenotypes[J]. Current Opinion in Microbiology, 2012, 15(5):577-582. [2] MARTINEZ J L, BAQUERO F, ANDERSSON D I. Predicting antibiotic resistance[J]. Nat Rev Microbiol, 2007, 5(12):958-965. [3] LUBY E, IBEKWE A M, ZILLES J, et al. Molecular methods for assessment of antibiotic resistance in agricultural ecosystems:Prospects and challenges[J]. Journal of Environmental Quality, 2016, 45(2):441-453. [4] RIZZO L, MANAIA C, MERLIN C, et al. Urban wastewater treatment plants as hotspots for antibiotic resistant bacteria and genes spread into the environment:A review[J]. Science of the Total Environment, 2013, 447:345-360. [5] SCHMIEDER R, EDWARDS R. Insights into antibiotic resistance through metagenomic approaches[J]. Future Microbiology, 2012, 7(1):73-89. [6] RODRIGUEZ-MOZAZ S, CHAMORRO S, MARTI E, et al. Occurrence of antibiotics and antibiotic resistance genes in hospital and urban wastewaters and their impact on the receiving river[J]. Water Research, 2015, 69:234-242. [7] SHARMA V K, JOHNSON N, CIZMAS L, et al. A review of the influence of treatment strategies on antibiotic resistant bacteria and antibiotic resistance genes[J]. Chemosphere, 2016, 150:702-714. [8] MA Y, METCH J W, YANG Y, et al. Shift in antibiotic resistance gene profiles associated with nanosilver during wastewater treatment[J]. FEMS Microbiology Ecology, 2016, 92(3):1-8(fiw022). [9] YANG Y, LI B, JU F, et al. Exploring variation of antibiotic resistance genes in activated sludge over a four-year period through a metagenomic approach[J]. Environmental Science & Technology, 2013, 47(18):10197-10205. [10] LIU B, POP M. Ardb-antibiotic resistance genes database[J]. Nucleic Acids Research, 2009, 37(suppl 1):D443-D447. [11] WANG Z, ZHANG X X, HUANG K, et al. Metagenomic profiling of antibiotic resistance genes and mobile genetic elements in a tannery wastewater treatment plant[J]. PloS One, 2013, 8(10):e76079. [12] GUPTA S K, PADMANABHAN B R, DIENE S M, et al. Arg-annot, a new bioinformatic tool to discover antibiotic resistance genes in bacterial genomes[J]. Antimicrobial Agents and Chemotherapy, 2014, 58(1):212-220. [13] HUGHES D, ANDERSSON D I. Selection of resistance at lethal and non-lethal antibiotic concentrations[J]. Current Opinion in Microbiology, 2012, 15(5):555-560. [14] ANDERSSON D I, HUGHES D. Microbiological effects of sublethal levels of antibiotics[J]. Nature Reviews Microbiology, 2014, 12(7):465-478. [15] RICHAUME A, ANGLE J S, SADOWSKY M J. Influence of soil variables on in situ plasmid transfer from escherichia coli to rhizobium fredii[J]. Applied and Environmental Microbiology, 1989, 55(7):1730-1734. [16] TOP E M, DE RORE H, COLLARD J M, et al. Retromobilization of heavy metal resistance genes in unpolluted and heavy metal polluted soil[J]. FEMS Microbiology Ecology, 1995, 18(3):191-203. [17] SØRENSEN S J, JENSEN L E. Transfer of plasmid rp4 in the spermosphere and rhizosphere of barley seedling[J]. Antonie van Leeuwenhoek, 1998, 73(1):69-77. [18] JUTKINA J, RUTGERSSON C, FLACH C F, et al. An assay for determining minimal concentrations of antibiotics that drive horizontal transfer of resistance[J]. Sci Total Environ, 2016, 548-549:131-138. [19] KLÜMPER U, RIBER L, DECHESNE A, et al. Broad host range plasmids can invade an unexpectedly diverse fraction of a soil bacterial community[J]. The ISME Journal, 2015, 9(4):934-945. [20] SZCZEPANOWSKI R, KRAHN I, BOHN N, et al. Novel macrolide resistance module carried by the incp-1β resistance plasmid prsb111, isolated from a wastewater treatment plant[J]. Antimicrobial Agents and Chemotherapy, 2007, 51(2):673-678. [21] ZHANG T, ZHANG X X, YE L. Plasmid metagenome reveals high levels of antibiotic resistance genes and mobile genetic elements in activated sludge[J]. PloS One, 2011, 6(10):e26041. [22] ADATO O, NINYO N, GOPHNA U, et al. Detecting horizontal gene transfer between closely related taxa[J]. PLoS Comput Biol, 2015, 11(10):e1004408. [23] TOP E M, SPRINGAEL D. The role of mobile genetic elements in bacterial adaptation to xenobiotic organic compounds[J]. Current Opinion in Biotechnology, 2003, 14(3):262-269. [24] SZCZEPANOWSKI R, LINKE B, KRAHN I, et al. Detection of 140 clinically relevant antibiotic-resistance genes in the plasmid metagenome of wastewater treatment plant bacteria showing reduced susceptibility to selected antibiotics[J]. Microbiology, 2009, 155(7):2306-2319. [25] 何基兵, 胡安谊, 陈猛, 等. 九龙江河口及厦门污水处理设施抗生素抗性基因污染分析[J]. 微生物学通报, 2012, 39(5):683-695. HE J B, HU A Y, CHEN M, et al. Studies on the pollution levels of antibiotic resistance genes in Jiulong River estuary and wastewater treatment plants in Xiamen[J]. Microbiology China, 2012, 39(5):683-695(in Chinese).
[26] ŁUCZKIEWICZ A, JANKOWSKA K, FUDALA-KSIA,EK S, et al. Antimicrobial resistance of fecal indicators in municipal wastewater treatment plant[J]. Water Research, 2010, 44(17):5089-5097. [27] 黄圣琳, 何势, 魏欣, 等. 污水处理厂中四环素类抗生素残留及其抗性基因污染特征研究进展[J]. 化工进展, 2015, 34(6):1779-1785. HUANG S L, HE S, WEI X, et al. Pollution characteristics of tetracycline residues and tetracycline resistance genes in sewage treatment plants:A review[J]. Chemical Industry and Engineering Progress, 2015, 34(6):1779-1785(in Chinese).
[28] BOUKI C, VENIERI D, DIAMADOPOULOS E. Detection and fate of antibiotic resistant bacteria in wastewater treatment plants:A review[J]. Ecotoxicology and Environmental Safety, 2013, 91:1-9. [29] FORSBERG K J, REYES A, WANG B, et al. The shared antibiotic resistome of soil bacteria and human pathogens[J]. Science, 2012, 337(6098):1107-1111. [30] KARKMAN A, JOHNSON T A, LYRA C, et al. High-throughput quantification of antibiotic resistance genes from an urban wastewater treatment plant[J]. FEMS Microbiol Ecol, 2016, 92(3):fiw014. [31] FORSBERG K J, PATEL S, GIBSON M K, et al. Bacterial phylogeny structures soil resistomes across habitats[J]. Nature, 2014, 509(7502):612-616. [32] MUNCK C, ALBERTSEN M, TELKE A, et al. Limited dissemination of the wastewater treatment plant core resistome[J]. Nature Communications, 2015, 6:8452-8461. [33] MA Y, WILSON C A, NOVAK J T, et al. Effect of various sludge digestion conditions on sulfonamide, macrolide, and tetracycline resistance genes and class I integrons[J]. Environmental Science & Technology, 2011, 45(18):7855-7861. [34] GILLINGS M R. Evolutionary consequences of antibiotic use for the resistome, mobilome, and microbial pangenome[J]. The Multiple Roles of Antibiotics and Antibiotic Resistance in Nature, 2013, 4:56-65. [35] BAHAROGLU Z, MAZEL D. Vibrio cholerae triggers SOS and mutagenesis in response to a wide range of antibiotics:A route towards multiresistance[J]. Antimicrobial Agents and Chemotherapy, 2011, 55(5):2438-2441. [36] TEZEL U, PAVLOSTATHIS S G. Quaternary ammonium disinfectants:Microbial adaptation, degradation and ecology[J]. Current Opinion in Biotechnology, 2015, 33:296-304. [37] BEABER J W, HOCHHUT B, WALDOR M K. Sos response promotes horizontal dissemination of antibiotic resistance genes[J]. Nature, 2004, 427(6969):72-74. [38] VON WINTERSDORFF C J, PENDERS J, VAN NIEKERK J M, et al. Dissemination of antimicrobial resistance in microbial ecosystems through horizontal gene transfer[J]. Front Microbiol, 2016, 7:173. [39] NORMAN A, HANSEN L H, SØRENSEN S J. Conjugative plasmids:Vessels of the communal gene pool[J]. Philosophical Transactions of the Royal Society B:Biological Sciences, 2009, 364(1527):2275-2289. [40] CAMBRAY G, GUEROUT A M, MAZEL D. Integrons[J]. Annual Review of Genetics, 2010, 44:141-166. [41] 覃彩霞, 佟娟, 申佩弘, 等. 污水处理过程中细菌整合子的研究进展[J]. 环境科学与技术, 2015, 38(11):1-7. QIN C X, TONG J, SHEN P H, et al. Integron-containing antibiotic resistant bacteria wastewater treatment processes:An overview[J]. Environmental Science & Technology, 2015, 38(11):1-7(in Chinese).
[42] STALDER T, BARRAUD O, JOVÉ T, et al. Quantitative and qualitative impact of hospital effluent on dissemination of the integron pool[J]. The ISME Journal, 2014, 8(4):768-777. [43] GAZE W H, ZHANG L, ABDOUSLAM N A, et al. Impacts of anthropogenic activity on the ecology of class 1 integrons and integron-associated genes in the environment[J]. The ISME Journal, 2011, 5(8):1253-1261. [44] BORRUSO L, HARMS K, JOHNSEN P J, et al. Distribution of class 1 integrons in a highly impacted catchment[J]. Science of the Total Environment, 2016, 566:1588-1594. [45] DI CESARE A, ECKERT E M, D'URSO S, et al. Co-occurrence of integrase 1, antibiotic and heavy metal resistance genes in municipal wastewater treatment plants[J]. Water Research, 2016, 94:208-214. [46] HU H W, WANG J T, LI J, et al. Field-based evidence for copper contamination induced changes of antibiotic resistance in agricultural soils[J]. Environ Microbiol, 2016, 18(11):3896-3909. [47] ZHANG Y, GU A Z, HE M, et al. Sub-inhibitory concentrations of disinfectants promote the horizontal transfer of multidrug resistance genes within and across genera[J]. Environmental Science & Technology, 2017, 51:570-580. [48] GETINO M, SANABRIA-RIOS D J, FERNANDEZ-LOPEZ R, et al. Synthetic fatty acids prevent plasmid-mediated horizontal gene transfer[J]. MBio, 2015, 6(5):e01032-01015. [49] ALAM M K, ALHHAZMI A, DECOTEAU J F, et al. RecA inhibitors potentiate antibiotic activity and block evolution of antibiotic resistance[J]. Cell Chem Biol, 2016, 23(3):381-391. -

计量
- 文章访问数: 1878
- HTML全文浏览数: 1735
- PDF下载数: 360
- 施引文献: 0