高级搜索

对比施用生物炭和肥料对土壤有效镉及酶活性的影响

,  ,  ,  ,  , 

downloadPDF

上一篇

下一篇

王秀梅, 安毅, 秦莉, 韩建华, 林大松, 霍莉莉. 对比施用生物炭和肥料对土壤有效镉及酶活性的影响[J]. 环境化学, 2018, 37(1): 67-74. doi: 10.7524/j.issn.0254-6108.2017050101
引用本文: 王秀梅, 安毅, 秦莉, 韩建华, 林大松, 霍莉莉. 对比施用生物炭和肥料对土壤有效镉及酶活性的影响[J]. 环境化学, 2018, 37(1): 67-74. doi: 10.7524/j.issn.0254-6108.2017050101
shu
WANG Xiumei, AN Yi, QIN Li, HAN Jianhua, LIN Dasong, HUO Lili. Effects of different fertilizers on Cd bioavailability and enzyme activity in soil[J]. Environmental Chemistry, 2018, 37(1): 67-74. doi: 10.7524/j.issn.0254-6108.2017050101
Citation: WANG Xiumei, AN Yi, QIN Li, HAN Jianhua, LIN Dasong, HUO Lili. Effects of different fertilizers on Cd bioavailability and enzyme activity in soil[J]. Environmental Chemistry, 2018, 37(1): 67-74. doi: 10.7524/j.issn.0254-6108.2017050101
shu

对比施用生物炭和肥料对土壤有效镉及酶活性的影响

  • 1.

    农业部环境保护科研监测所,天津, 300191;

  • 2.

    天津市农业环境保护管理监测站, 天津, 300036

  • 基金项目:

    公益性行业（农业）科研专项"农产品产地重金属污染安全评估技术与设备研制"（201403014）和中国农业科学院创新工程项目（2016-cxgc-lyj）资助.

Effects of different fertilizers on Cd bioavailability and enzyme activity in soil

  • 1.

    Agro-Environmental Protection Institute, Ministry of Agriculture, Tianjin, 300191, China;

  • 2.

    Tianjin Agri-Environmental Monitoring Station, Tianjin, 300036, China

  • Fund Project: Supported by the Special Fund for Agro-Scientific Research in the Public Interest (201403014) and Innovative Engineering Project of Chinese Academy of Agricultural Sciences (2016-cxgc-lyj).

  • 摘要: 采用盆栽试验，研究了施用有机肥、菌肥、海藻肥及生物炭对土壤性质、土壤有效态镉含量、土壤酶活性、油菜Cd含量的影响.研究表明，使用4种修复材料均可降低土壤pH，增加土壤中有机质、速效N、速效P、速效K的含量；4种不同的修复材料均可降低土壤有效态镉含量及油菜中镉含量，其中有机肥的作用最为显著，可使土壤及油菜的镉含量分别降低25.5%-42.5%和16.4%-27.5%.添加有机肥、生物炭、菌肥可使土壤过氧化氢酶活性显著增加，而海藻肥的加入则降低了土壤过氧化氢酶活性.有机肥加入对过氧化氢酶的增加效果最为显著，增幅达34.2%-73.3%.有机肥可以使土壤磷酸酶活性降低22.2%-40.0%，而生物炭和菌肥对磷酸酶的活性无显著性影响.海藻肥低浓度时，土壤磷酸酶活性下降，高浓度时其活性增加.有机肥、菌肥、海藻肥的施入均可显著增加蔗糖酶的活性，而生物炭的施入会降低蔗糖酶活性.通过对土壤有效Cd、3种酶活性与土壤理化性质的相关性分析发现，土壤过氧化氢酶和磷酸酶活性与土壤速效N的含量在0.01水平上显著相关.
    Abstract: Pot experiments were conducted to investigate the effects of organic fertilizers, bacterial fertilizers, algae fertilizers, and biochars on the physicochemical properties of soil, Cd aviailability, enzyme activities and Cd content in rape. The results showed that all the four soil amendments decreased soil pH, but increased the contents of soil organic matter, available N, available P and available K. Also, they reduced the available Cd content in soil and rape. With addition of the organic fertilizer, the available Cd content in soil and rape decreased by 25.5%-42.5% and 16.4%-27.5%, respectively. The catalase activity in soil increased significantly by the organic fertilizer, biochar, and bacterial fertilizer, while decreased by the algae fertilizer. Organic fertilizer is the most significant factor in increasing catalase activity, with an increase of 34.2%-73.3%. Conversely, the organic fertilizer decreased phosphatase activity by 22.2%-40.0%. However, the addition of biochar and bacterial fertilizer had insignificant effect on phosphatase activity. Low-dose (80 g·kg-1) algae fertilizer decreased the phosphatase activity, but increased its activity at a high-dose (240 g·kg-1). The application of organic fertilizer, bacteria fertilizer, and algae fertilizer increased the activity of sucrase, whereas biochar reduced the activity of sucrase. The correlation analyses between the soil physicochemical properties and the available Cd content of soil or enzyme activities showed that the catalase activity and phosphatase activity were significantly correlated with available N content in soil(P<0.01).
  • 加载中
  • [1] 环境保护部, 国土资源部. 全国土壤污染状况调查公报[R]. 中国环保产业, 2014(5):10-11. Ministry of Environment Protection, Ministry of Land and Resources. The national soil pollution condition investigation communique[R]. China Environmental Protection Industry, 2014

    (5):10-11(in Chinese).

    [2] 李玉浸. 集约化农业的环境问题与对策[M]. 北京:中国农业出版社, 2001:57-82. LI Y J. Environmental problems and countermeasures of intensive agriculture[M]. Beijing:China Agriculture Press, 2001:57

    -82(in Chinese).

    [3] AKESSON A, JULIN B, WOLK A. Long-term dietary cadmium intake and postmenopausal endometrial cancer incidence:A population-based prospective cohort study[J]. Cancer Research, 2008, 68(15):6435-6441.
    [4] JALLOH M A, CHEN J, ZHEN F, et al. Effect of different N fertilizer forms on antioxidant capacity and grain yield of rice growing under Cd stress.[J]. Journal of Hazardous Materials, 2009, 162(2/3):1081-1085.
    [5] 李剑睿, 徐应明, 林大松,等. 农田重金属污染原位钝化修复研究进展[J]. 生态环境学报, 2014(4):721-728. LI J R, XU Y M, LIN D S, et al. In situ immobilization remediation of heavy metals in contaminated soils:A review[J]. Ecology and Environmental Sciences, 2014

    (4):721-728(in Chinese).

    [6] LI T, DI Z, YANG X, et al. Effects of dissolved organic matter from the rhizosphere of the hyperaccumulator Sedum alfredii, on sorption of zinc and cadmium by different soils[J]. Journal of Hazardous Materials, 2011, 192(3):1616-1622.
    [7] SHANG Y J, WANG H B, SHI J. Effects of straw biomass charcoal on enzyme activity in Cd contaminated soil[J]. Journal of Agricultural Resources & Environment, 2015,32(1):20-25.
    [8] 王强, 石伟勇. 海藻肥对番茄生长的影响及其机理研究[J]. 浙江农业科学, 2003, 1(2):67-70.

    WANG Q, SHI W Y. Study on effects and mechanism of seaweed on growth of tomato plants[J]. Journal of Zhejiang Agricultural Science, 2003, 1(2):67-70(in Chinese).

    [9] 高译丹, 梁成华, 裴中健,等. 施用生物炭和石灰对土壤镉形态转化的影响[J]. 水土保持学报, 2014, 28(2):258-261.

    GAO Y D, LIANG C H, PEI Z J, et al. Effects of biochar and lime on the fraction transform of cadmium in contaminated soil[J]. Journal of Soil and Water Conservation, 2014, 28(2):258-261(in Chinese).

    [10] 李发生, 韩梅, 熊代群,等. 不同浸提剂对几种典型土壤中重金属有效态的浸提效率研究[J]. 农业环境科学学报, 2003, 22(6):704-706.

    LI F S, HAN M, XIONG D Q, et al. Efficiency of some extractants for available heavy metals from several typical soils[J]. Journal of Agro-Environment Science, 2003, 22(6):704-706(in Chinese).

    [11] WEI S H, ZHOU Q X. Identification of weed species with hyperaccumulative characteristics of heavy metals[J]. Progress in Natural Science:Materials International, 2004,14(6):495-503.
    [12] 关松荫. 土壤酶及其研究方法[M]. 北京:中国农业出版社, 1987 GUAN S Y. Soil enzyme and it's research methods[M]. Beijing:China Agriculture Press, 1987(in Chinese).
    [13] ZHAO Z P, YAN S, LIU F, et al. Effects of chemical fertilizer combined with organic manure on Fuji apple quality, yield and soil fertility in apple orchard on the Loess Plateau of China[J]. International Journal of Agricultural & Biological Engineering, 2014, 7(2):45-55.
    [14] 朱丹, 张磊, 韦泽秀,等. 菌肥对青稞根际土壤理化性质以及微生物群落的影响[J]. 土壤学报, 2014,51(3):627-637.

    ZHU D, ZHANG L, WEI Z X, et al. Effects of bacterial manure on soil physicochemical properties and microbial community diversity in rhizosphere of highland barley[J].Acta Pedologica Sinica,2014,51(3):627-637(in Chinese).

    [15] 韩光, 张磊, 邱勤,等. 复合型PGPR和苜蓿对新垦地土壤培肥效果研究[J]. 土壤学报, 2011, 48(2):405-411.

    HAN G, ZHANG L, QIU Q, et al. Effects of PGPR and alfalfa on soil building of newly-reclaimed land[J]. Acta Pedologica Sinica,2011,48(2):405-411(in Chinese).

    [16] 保万魁. 海藻提取物与铜铁硼配施对小油菜营养特性的影响[D].北京:中国农业科学院, 2008. BAO W K. Effect of rape nutrition characteristics on sea extract combined with Fe, Cu, B application[D]. Beijing:Chinese Academy of Agricultural Sciences, 2008(in Chinese).
    [17] RATHORE S S, CHAhaudhary D R, Boricha G N, et al. Effect of seaweed extract on the growth, yield and nutrient uptake of soybean (Glycine max) under rainfed conditions[J]. South African Journal of Botany, 2009, 75(2):351-355.
    [18] 李智卫. 海藻对不同促生菌生长的影响及应用[D]. 泰安:山东农业大学, 2010. LI Z W. Influence of seaweed on different plant-growth promoting microorganisms and its application[D]. Tai'an:Shandong Agricultural University, 2010(in Chinese).
    [19] 周桂玉, 窦森, 刘世杰. 生物质炭结构性质及其对土壤有效养分和腐殖质组成的影响[J]. 农业环境科学学报, 2011, 30(10):2075-2080.

    ZHOU G Y, DOU S, LIU S J. The structural characteristics of biochar and its effect on soil available nutrients and humus composition[J]. Journal of Agro-Environment Science, 2011, 30(10):2075-2080(in Chinese).

    [20] TAGHIZADEHTOOSI A, CLOUGH T J, SHERLOCK R R, et al. Biochar adsorbed ammonia is bioavailable[J]. Plant and Soil, 2012, 350(1):57-69.
    [21] YUAN J H, XU R K. The amelioration effects of low temperature biochar generated from nine crop residues on an acidi cultisol[J]. Soil Use and Management,2011,27:110-115.
    [22] 陈灿, 潘亚男, 王欣,等. 凤眼莲生物炭对稻田土壤肥力的影响[J]. 环境化学, 2017, 36(4):907-914.

    CHEN C, PAN Y N, WANG X, et al. Influence of water hyacinth biochar on retention of nutrition in paddy soils[J]. Environmental Chemistry, 2017, 36(4):907-914(in Chinese).

    [23] 王健鹂, 李阿红, 王会志. 有机肥对土壤理化性质的影响[J]. 吉林蔬菜, 2007(4):51-53. WANG J P, LI A H, WANG H Z. Effects of organic fertilizer on soil physicochemical properties[J]. Jilin Vegetable, 2007

    (4):51-53(in Chinese).

    [24] TIAN X, LI T, YANG K, et al. Effect of humic acids on physicochemical property and Cd(Ⅱ) sorption of multiwalled carbon nanotubes[J]. Chemosphere, 2012, 89(11):1316-1322.
    [25] 华珞, 陈世宝. 有机肥对镉锌污染土壤的改良效应[J]. 农业环境科学学报, 1998, 17(2):55-59.

    HUA L, CHEN S B. Amelioration of soils polluted by cadmium and zinc by organic matter[J]. Journal of Agriculture Environment Science, 1998,17(2):55-59(in Chinese).

    [26] 曹书苗. 放线菌强化植物修复土壤铅镉污染的效应及机理[D]. 西安:长安大学, 2016. CAO S M. Actinomycetes enhance phytoremediation of lead and cadmium contaminated soil and its mechanisms[D]. Xi'an:Chang'an University, 2016(in Chinese).
    [27] MÉNDEZ A, GÍMEZ A, PAZ-FERREIRO J, et al. Effects of sewage sludge biochar on plant metal availability after application to a Mediterranean soil[J]. Chemosphere, 2012, 89(11):1354-1359.
    [28] BEESLEY L, MARMIROLI M. The immobilisation and retention of soluble arsenic, cadmium and zinc by biochar[J]. Environmental Pollution, 2011, 159(2):474-480(in Chinese).
    [29] LU H, ZHANG W, YANG Y, et al. Relative distribution of Pb 2+, sorption mechanisms by sludge-derived biochar[J]. Water Research, 2012, 46(3):854-862.
    [30]
    [31] 杨苞梅, 李国良, 姚丽贤, 等. 有机肥施用模式对蔬菜产量、品质及土壤酶活性的影响[J]. 土壤通报, 2011, 42(1):70-76.

    YANG B M, LI Liang G, YAO Li X, et al. effects of organic fertilizer application pattern on the vegetable yield, quality and soil enzyme activity[J]. Chinese Journal of Soil Science, 2011, 42(1):70-76(in Chinese).

    [32] 荆瑞勇, 王丽艳, 郭永霞. 生物有机肥对盆栽小白菜土壤酶活性和微生物数量的影响[J]. 水土保持研究, 2015, 22(2):79-83.

    JING R Y, WANG L Y, GUO Y X. Effects of bioorganic fertilizers on soil enzyme activities and amount of microorganism in pakchoi pot experiment[J]. Research of Soil and Water Conservation, 2015, 22(2):79-83(in Chinese).

    [33] 李金岚, 王红芬, 洪坚平. 生物菌肥对采煤沉陷区复垦土壤酶活性的影响[J]. 山西农业科学, 2010, 38(2):53-54.

    LI J L, WANG H F, HONG J P. Effects of bacterial manure on soil enzymatic activity in core-mining subsidence area[J]. Journal of Shanxi Agricultural Sciences, 2010, 38(2):53-54(in Chinese).

    [34] LI F, LIU M, LI Z, et al. Changes in soil microbial biomass and functional diversity with a nitrogen gradient in soil columns[J]. Applied Soil Ecology, 2013, 64(1):1-6.
    [35] GLASER B, LEHMANN J, ZECH W. Ameliorating physical and chemical properties of highly weathered soils in the tropics with charcoal-a review[J]. Biology and Fertility of Soils, 2002, 35(4):219-230.
    [36] 王震宇, 徐振华, 郑浩,等. 花生壳生物炭对中国北方典型果园酸化土壤改性研究[J]. 中国海洋大学学报自然科学版, 2013, 43(8):86-91.

    WANG Z Y, XU Z H, ZHENG H, et al. Effect of peanut hull biochar on amelioration of typical orchard acidic soil in northern China[J]. Periodical of Ocean University of China, 2013, 43(8):86-91(in Chinese).

    [37] HAMER U, MARSCHNER B, BRODOWSKI S, et al. Interactive priming of black carbon and glucose mineralisation[J]. Organic Geochemistry, 2004, 35(7):823-830.
    [38] YANG X, LIU J, MCGROUTHER K, et al. Effect of biochar on the extractability of heavy metals (Cd, Cu, Pb, and Zn) and enzyme activity in soil[J]. Environmental Science and Pollution Research, 2016, 23(2):974-984.
    [39] LI Z Y, TANG S, DENG X F, et al. Contrasting effects of elevated CO2 on Cu and Cd uptake by different rice varieties grown on contaminated soils with two levels of metals:Implication for phytoextraction and food safety[J]. Hazard Mater, 2010, 177:352-361.
    [40] SALATI S, QUADRI G, TAMBONE F, et al. Fresh organic matter of municipal solid waste enhances phytoextraction of heavy metals from contaminated soil[J]. Environmental Pollution, 2010, 158(5):1899-1906.
    [41] XUE T, WANG J, SUN X. Variation in enzymes activities of rhizospheric substrate and influencing factors during nursing of watermelon seedlings[J]. Agricul Tural Science & Technology, 2015, 16(7):1321.
  • 加载中
WeChat 点击查看大图
计量
  • 文章访问数:  1181
  • HTML全文浏览数:  1103
  • PDF下载数:  268
  • 施引文献:  0
出版历程
  • 收稿日期:  2017-05-01
  • 刊出日期:  2018-01-15
王秀梅, 安毅, 秦莉, 韩建华, 林大松, 霍莉莉. 对比施用生物炭和肥料对土壤有效镉及酶活性的影响[J]. 环境化学, 2018, 37(1): 67-74. doi: 10.7524/j.issn.0254-6108.2017050101
引用本文: 王秀梅, 安毅, 秦莉, 韩建华, 林大松, 霍莉莉. 对比施用生物炭和肥料对土壤有效镉及酶活性的影响[J]. 环境化学, 2018, 37(1): 67-74. doi: 10.7524/j.issn.0254-6108.2017050101
shu
WANG Xiumei, AN Yi, QIN Li, HAN Jianhua, LIN Dasong, HUO Lili. Effects of different fertilizers on Cd bioavailability and enzyme activity in soil[J]. Environmental Chemistry, 2018, 37(1): 67-74. doi: 10.7524/j.issn.0254-6108.2017050101
Citation: WANG Xiumei, AN Yi, QIN Li, HAN Jianhua, LIN Dasong, HUO Lili. Effects of different fertilizers on Cd bioavailability and enzyme activity in soil[J]. Environmental Chemistry, 2018, 37(1): 67-74. doi: 10.7524/j.issn.0254-6108.2017050101
shu

对比施用生物炭和肥料对土壤有效镉及酶活性的影响

  • 1.  农业部环境保护科研监测所,天津, 300191;
  • 2.  天津市农业环境保护管理监测站, 天津, 300036
  • 收稿日期:  2017-05-01
基金项目:

公益性行业（农业）科研专项"农产品产地重金属污染安全评估技术与设备研制"（201403014）和中国农业科学院创新工程项目（2016-cxgc-lyj）资助.

摘要: 采用盆栽试验，研究了施用有机肥、菌肥、海藻肥及生物炭对土壤性质、土壤有效态镉含量、土壤酶活性、油菜Cd含量的影响.研究表明，使用4种修复材料均可降低土壤pH，增加土壤中有机质、速效N、速效P、速效K的含量；4种不同的修复材料均可降低土壤有效态镉含量及油菜中镉含量，其中有机肥的作用最为显著，可使土壤及油菜的镉含量分别降低25.5%-42.5%和16.4%-27.5%.添加有机肥、生物炭、菌肥可使土壤过氧化氢酶活性显著增加，而海藻肥的加入则降低了土壤过氧化氢酶活性.有机肥加入对过氧化氢酶的增加效果最为显著，增幅达34.2%-73.3%.有机肥可以使土壤磷酸酶活性降低22.2%-40.0%，而生物炭和菌肥对磷酸酶的活性无显著性影响.海藻肥低浓度时，土壤磷酸酶活性下降，高浓度时其活性增加.有机肥、菌肥、海藻肥的施入均可显著增加蔗糖酶的活性，而生物炭的施入会降低蔗糖酶活性.通过对土壤有效Cd、3种酶活性与土壤理化性质的相关性分析发现，土壤过氧化氢酶和磷酸酶活性与土壤速效N的含量在0.01水平上显著相关.

English Abstract

    全文HTML

参考文献 (41)

返回顶部

目录

/

返回文章
返回

Copyright © 2019 中国科学院生态环境研究中心