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胞内砷磷含量和比值对莱茵衣藻砷酸盐和亚砷酸盐耐性的影响

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郑燕恒, 李颢, 张春华, 葛滢. 胞内砷磷含量和比值对莱茵衣藻砷酸盐和亚砷酸盐耐性的影响[J]. 环境化学, 2018, 37(1): 75-81. doi: 10.7524/j.issn.0254-6108.2017040702
引用本文: 郑燕恒, 李颢, 张春华, 葛滢. 胞内砷磷含量和比值对莱茵衣藻砷酸盐和亚砷酸盐耐性的影响[J]. 环境化学, 2018, 37(1): 75-81. doi: 10.7524/j.issn.0254-6108.2017040702
shu
ZHENG Yanheng, LI Hao, ZHANG Chunhua, GE Ying. Effects of intracellular arsenic and phosphorus content and ratio on the tolerance of arsenate and arsenite in Chlamydomonas reinhardtii[J]. Environmental Chemistry, 2018, 37(1): 75-81. doi: 10.7524/j.issn.0254-6108.2017040702
Citation: ZHENG Yanheng, LI Hao, ZHANG Chunhua, GE Ying. Effects of intracellular arsenic and phosphorus content and ratio on the tolerance of arsenate and arsenite in Chlamydomonas reinhardtii[J]. Environmental Chemistry, 2018, 37(1): 75-81. doi: 10.7524/j.issn.0254-6108.2017040702
shu

胞内砷磷含量和比值对莱茵衣藻砷酸盐和亚砷酸盐耐性的影响

  • 1.

    南京农业大学资源与环境科学学院,江苏省海洋生物学重点实验室, 南京, 210095;

  • 2.

    南京农业大学生命科学实验中心,元素与生命科学示范实验室, 南京, 210095

  • 基金项目:

    国家自然科学基金（41371468）资助.

Effects of intracellular arsenic and phosphorus content and ratio on the tolerance of arsenate and arsenite in Chlamydomonas reinhardtii

  • 1.

    College of Resource and Environmental Sciences, Jiangsu Provincial Key Laboratory of Marine Biology, Nanjing Agricultural University, Nanjing, 210095, China;

  • 2.

    Demonstration Laboratory of Element and Life Science, Laboratory Centre of Life Science, Nanjing Agricultural University, Nanjing, 210095, China

  • Fund Project: Supported by the National Natural Science Foundation of China (41371468).

  • 摘要: 为探索胞内砷（As）与磷（P）含量和比值与莱茵衣藻As耐性的关系，本文设置两个磷酸盐（PO43-）和系列砷酸盐（AsV））、亚砷酸盐（AsⅢ））浓度，处理72 h后测定莱茵衣藻生长情况和胞内As、P含量，并以培养液As浓度（[As]dis）、胞内As含量（[As]intra）、胞内砷磷比（[As：P]intra）推算半数效应浓度（EC50），比较这3种指标对莱茵衣藻As耐性的评价效果.结果表明，随着[As]dis的增加，莱茵衣藻[As]intra上升，提高培养液PO43-浓度不影响AsⅢ）处理下的[As]intra，但显著降低了AsV）处理下的[As]intra.以[As]dis表征EC50时，两种PO43-水平（0.315、3.15 mg·L-1）下的AsⅢ）-EC50（2090.3、21183.6 μg·L-1-As）明显高于AsV）-EC50（162.1、2358.3 μg·L-1-As）.基于[As]intra的EC50数据显示，PO43-水平不影响AsⅢ）-EC50（123.9、125.0 μg·g-1-As-dw），但显著影响AsV）-EC50（7.4、58.6 μg·g-1-As-dw）.由[As：P]intra推算的EC50可知，PO43-对两种形态As毒性的影响相反，AsⅢ）-EC50分别为21.1、6.1（mol/mol，As/P），AsV）-EC50分别为1.3、3.4（mol/mol，As/P）.研究结果说明，AsV）对该藻的毒性大于AsⅢ），莱茵衣藻对AsV）和AsⅢ）的耐性除了受到培养液PO43-浓度的制约外，还受到胞内As、P含量及其比值的影响.
    Abstract: In order to explore the relationship between intracellular arsenic(As) and phosphorus(P) content and their ratios with As tolerance in Chlamydomonas reinhardtii, two phosphate (PO43-) levels and a series of arsenate (As(V)) and arsenite (As(Ⅲ)) concentrations were set up, and the growth of C. reinhardtii and the contents of As and P in the C. reinhardtii cells after 72 h treatment were measured. The half maximal effective concentration values (EC50) based on the As concentration in growth media ([As]dis), the intracellular arsenic content ([As]intra) and the ratio of intracellular arsenic to phosphorus([As:P]intra) were calculated. Results showed that the[As]intra of C. reinhardtii increased with the increase of[As]dis. Higher level of PO43- in the culture did not affect the[As]intra under As (Ⅲ) treatment, but significantly decreased it under As(V) treatment. When EC50 is characterized by[As]dis, As(Ⅲ)-EC50 (2090.3, 21183.6 μg·L-1-As under two PO43- levels) were significantly higher than the As(V)-EC50 (162.1, 2358.3 μg·L-1-As). The data of[As]intra EC50 showed that PO43- level did not affect As(Ⅲ)-EC50 (123.9, 125.0 μg·g-1-As-dw), but significantly affected As(V)-EC50 (7.4, 58.6 μg·g-1-As-dw). From the EC50 derived from[As:P]intra, we found that the effect of PO43- on the tolerance of two As species was opposite. The As(Ⅲ)-EC50 were 21.1 and 6.1(mol/mol, As/P), while As(V)-EC50 were 1.3 and 3.4(mol/mol, As/P). Taken together, these data indicated that As(V) was more toxic to the microalgae than As(Ⅲ). The tolerance of As(Ⅴ) and As (Ⅲ) by C. reinhardtii was not only affected by the PO43- concentration in the culture, but also affected by intracellular As and P content and their ratios.
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  • 收稿日期:  2017-04-07
  • 刊出日期:  2018-01-15
郑燕恒, 李颢, 张春华, 葛滢. 胞内砷磷含量和比值对莱茵衣藻砷酸盐和亚砷酸盐耐性的影响[J]. 环境化学, 2018, 37(1): 75-81. doi: 10.7524/j.issn.0254-6108.2017040702
引用本文: 郑燕恒, 李颢, 张春华, 葛滢. 胞内砷磷含量和比值对莱茵衣藻砷酸盐和亚砷酸盐耐性的影响[J]. 环境化学, 2018, 37(1): 75-81. doi: 10.7524/j.issn.0254-6108.2017040702
shu
ZHENG Yanheng, LI Hao, ZHANG Chunhua, GE Ying. Effects of intracellular arsenic and phosphorus content and ratio on the tolerance of arsenate and arsenite in Chlamydomonas reinhardtii[J]. Environmental Chemistry, 2018, 37(1): 75-81. doi: 10.7524/j.issn.0254-6108.2017040702
Citation: ZHENG Yanheng, LI Hao, ZHANG Chunhua, GE Ying. Effects of intracellular arsenic and phosphorus content and ratio on the tolerance of arsenate and arsenite in Chlamydomonas reinhardtii[J]. Environmental Chemistry, 2018, 37(1): 75-81. doi: 10.7524/j.issn.0254-6108.2017040702
shu

胞内砷磷含量和比值对莱茵衣藻砷酸盐和亚砷酸盐耐性的影响

  • 1.  南京农业大学资源与环境科学学院,江苏省海洋生物学重点实验室, 南京, 210095;
  • 2.  南京农业大学生命科学实验中心,元素与生命科学示范实验室, 南京, 210095
  • 收稿日期:  2017-04-07
基金项目:

国家自然科学基金（41371468）资助.

摘要: 为探索胞内砷（As）与磷（P）含量和比值与莱茵衣藻As耐性的关系，本文设置两个磷酸盐（PO43-）和系列砷酸盐（AsV））、亚砷酸盐（AsⅢ））浓度，处理72 h后测定莱茵衣藻生长情况和胞内As、P含量，并以培养液As浓度（[As]dis）、胞内As含量（[As]intra）、胞内砷磷比（[As：P]intra）推算半数效应浓度（EC50），比较这3种指标对莱茵衣藻As耐性的评价效果.结果表明，随着[As]dis的增加，莱茵衣藻[As]intra上升，提高培养液PO43-浓度不影响AsⅢ）处理下的[As]intra，但显著降低了AsV）处理下的[As]intra.以[As]dis表征EC50时，两种PO43-水平（0.315、3.15 mg·L-1）下的AsⅢ）-EC50（2090.3、21183.6 μg·L-1-As）明显高于AsV）-EC50（162.1、2358.3 μg·L-1-As）.基于[As]intra的EC50数据显示，PO43-水平不影响AsⅢ）-EC50（123.9、125.0 μg·g-1-As-dw），但显著影响AsV）-EC50（7.4、58.6 μg·g-1-As-dw）.由[As：P]intra推算的EC50可知，PO43-对两种形态As毒性的影响相反，AsⅢ）-EC50分别为21.1、6.1（mol/mol，As/P），AsV）-EC50分别为1.3、3.4（mol/mol，As/P）.研究结果说明，AsV）对该藻的毒性大于AsⅢ），莱茵衣藻对AsV）和AsⅢ）的耐性除了受到培养液PO43-浓度的制约外，还受到胞内As、P含量及其比值的影响.

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