高级搜索

环境相关浓度铅暴露诱导斑马鱼仔鱼神经行为毒性

,  ,  ,  , 

downloadPDF

上一篇

下一篇

张晴, 张斌, 赵静, 白建峰, 尹大强. 环境相关浓度铅暴露诱导斑马鱼仔鱼神经行为毒性[J]. 环境化学, 2018, 37(3): 445-452. doi: 10.7524/j.issn.0254-6108.2017080905
引用本文: 张晴, 张斌, 赵静, 白建峰, 尹大强. 环境相关浓度铅暴露诱导斑马鱼仔鱼神经行为毒性[J]. 环境化学, 2018, 37(3): 445-452. doi: 10.7524/j.issn.0254-6108.2017080905
shu
ZHANG Qing, ZHANG Bin, ZHAO Jing, BAI Jianfeng, YIN Daqiang. Neurobehavioral toxicity of zebrafish larvae caused by lead exposure at environmentally relevant concentrations[J]. Environmental Chemistry, 2018, 37(3): 445-452. doi: 10.7524/j.issn.0254-6108.2017080905
Citation: ZHANG Qing, ZHANG Bin, ZHAO Jing, BAI Jianfeng, YIN Daqiang. Neurobehavioral toxicity of zebrafish larvae caused by lead exposure at environmentally relevant concentrations[J]. Environmental Chemistry, 2018, 37(3): 445-452. doi: 10.7524/j.issn.0254-6108.2017080905
shu

环境相关浓度铅暴露诱导斑马鱼仔鱼神经行为毒性

  • 1.

    上海第二工业大学电子废弃物研究中心, 上海电子废弃物资源化协同创新中心, 上海, 201209;

  • 2.

    同济大学环境科学与工程学院, 长江水环境教育部重点实验室, 上海, 200092

  • 基金项目:

    国家自然科学基金（21507080，21577104），上海第二工业大学重点学科（XXKZD1602）和上海第二工业大学研究生项目基金（EGD16YJ046）资助.

Neurobehavioral toxicity of zebrafish larvae caused by lead exposure at environmentally relevant concentrations

  • 1.

    WEEE Research Centre of Shanghai Polytechnic University, Shanghai Collaborative Innovation Centre for WEEE Recycling, Shanghai, 201209, China;

  • 2.

    Key Laboratory of Yangtze River Water Environment, Ministry of Education, College of Environmental Science & Engineering, Tongji University, Shanghai, 200092, China

  • Fund Project: Supported by the National Natural Science Foundation of China(21507080, 21577104), the Key Subject of Shanghai Polytechnic University(XXKZD1602) and the Graduate Program Fund of Shanghai Polytechnic University (EGD16YJ046).

  • 摘要: 铅是公认的神经毒物，它的神经毒性是全世界备受关注的重要公共健康问题，特别是发育早期阶段铅暴露与神经行为变化之间的关联.斑马鱼被认为是研究发育早期阶段低浓度铅暴露诱导神经行为毒性的优良模式生物.因此，本研究以斑马鱼胚胎/仔鱼为研究对象，利用Zebrabox建立的行为学效应测试方法，包括运动行为、转动行为和社交行为，系统评价发育早期阶段环境相关浓度（以电子废弃物拆解场地为例）铅暴露的神经行为毒性效应.发育正常的斑马鱼胚胎暴露在浓度为0、5、10、20 μg·L-1铅暴露液中直至6 dpf.铅暴露对斑马鱼胚胎/仔鱼具有发育毒性作用，包括孵化率和存活率下降，卵黄囊肿、心包囊肿和脊柱弯曲等畸形现象.在光照条件下，铅暴露对斑马鱼仔鱼的运行行为和转向行为并未产生显著影响；但是在黑暗条件下，20 μg·L-1铅暴露后斑马鱼仔鱼运动距离和总转向次数均显著下降，但是其行进路径角度和偏转偏好却无变化.与运动行为和转向行为不同，在黑暗条件下，铅暴露对斑马鱼仔鱼的社交行为并未产生显著影响；但在光照条件下20 μg·L-1铅暴露却显著改变了仔鱼的社交次数.本研究结果表明，行为学指标不仅具备足够的灵敏度可用于指示电子废弃物拆解场地铅污染的潜在风险，还能更好地服务于人类健康早期预警的需求.
    Abstract: Lead (Pb) is recognized as a neurological toxicant. Up to date, Pb neurotoxicity continues to be a major public health problem around the world,especially the link between early developmental Pb exposure and neurological alterations. Zebrafish is considered as a useful model for the identification of the association between early-life low-dose Pb exposure and neurological adverse outcomes. In this study zebrafish embryos/larvae were used as research object and the test methods of the behavioral effects including locomotor activity, rotation activity and social activity were established with Zebrabox, which can systematically evaluate the neurobehavioral toxicity of early-life low-dose Pb exposure (referred from e-waste disposal sites). The normal zebrafish embryos were selected and exposed to Pb at the concentrations of 0, 5, 10,20 μg·L-1 until 6 dpf. The developmental toxicity of zebrafish embryos/larvae was induced by Pb exposure, including the decline of hatching rates and survival rates, and the malformation of yolk-edema, pericardial edema and axial spinal curvature. Pb exposure had no significant impact on the locomotor activity and rotation activity of the zebrafish larvae during the light illumination period. However, in the dark conditions, the locomotor distance and the total rotation times of zebrafish larvae were decreased significantly after exposume to 20 μg·L-1 Pb, and the path angle and orientation preference did not change. Different from the locomotor activity and rotation activity, in the dark conditions, Pb exposure had no significant impact on the social activity of zebrafish larvae. However, the social activity especially the contact times of zebrafish larvae were changed significantly with 20 μg·L-1 Pb exposure. Our results suggest that the behavioral indicators are not only sensitive enough to evaluate the potential risk of Pb pollution at e-waste disposal sites, but can also be applied to establish novel early warning system for health risk assessment.
  • 加载中
  • [1] DUAN H B, HU J K, TAN Q J, et al. Systematic characterization of generation and management of e-waste in China[J]. Environmental Science and Pollution Research, 2016, 23(2):1929-1943.
    [2] ZENG X L, GONG R Y, CHEN W Q, et al. Uncovering the recycling potential of "New" WEEE in China[J]. Environmental Science and Technology, 2016,50(3):1347-1358.
    [3] QIU Y W, LIN D, LIU J Q, et al. Bioaccumulation of trace metals in farmed fish from South China and potential risk assessment[J]. Ecotoxicology and Environmental Safety, 2011, 74(3):284-293.
    [4] ZHANG Q, YE J J, CHEN J Y, et al. Risk assessment of polychlorinated biphenyls and heavy metals in soils of an abandoned e-waste site in China[J]. Environmental Pollution, 2014, 185:258-265.
    [5] WU Q H, LEUNG J Y, GENG X H, et al. Heavy metal contamination of soil and water in the vicinity of an abandoned e-waste recycling site:Implications for dissemination of heavy metals[J]. Science of the Total Environment, 2015, 506:217-225.
    [6] HUO X, PENG L, XU X J, et al. Elevated blood lead levels of children in Guiyu, an electronic waste recycling town in China[J]. Environmental Health Perspectives, 2007, 115(7):1113-1117.
    [7] ZHENG L K, WU K S, LI Y, et al. Blood lead and cadmium levels and relevant factors among children from an e-waste recycling town in China[J]. Environmental Research, 2008, 108:15-20.
    [8] 赵静, 朱祥伟, 徐挺, 等. BDE17及OH-BDE17暴露对斑马鱼幼鱼的运动行为效应[J]. 环境化学, 2015, 34(7):1239-1245.

    ZHAO J, ZHU X W, XU T, et al. Locomotor behavior effect in zebrafish larvae after exposure to BDE17 and OH-BDE17[J]. Environmental Chemistry, 2015, 34(7):1239-1245(in Chinese).

    [9] VALERIA D G S, ADILSON P S, JHONNES M S T, et al. Metabolic and behavior changes in surubim acutely exposed to a glyphosate-based herbicide[J]. Archives of Environmental Contamination and Toxicology, 2014, 67(4):659-667.
    [10] IRONS T D, MACPHAIL R C, HUNTER D L, et al. Acute neuroactive drug exposures alter locomotor activity in larval zebrafish[J]. Neurotoxicology and Teratology, 2010, 32(1):84-90.
    [11] ABDULMAJEED W I, SULIEMAN H B, ZUBAYR M O, et al. Honey prevents neurobehavioural deficit and oxidative stress induced by lead acetate exposure in male wistar rats:A preliminary study[J]. Metabolic Brain Disease, 2016, 31(1):37-44.
    [12] AKTAR S, JAHAN M, ALAM S, et al. Individual and combined effects of arsenic and lead on behavioral and biochemical changes in mice[J]. Biological Trace Element Research, 2017, 177(2):288-296.
    [13] MANSOURI M T, CAULI O. Motor alterations induced by chronic lead exposure[J]. Environmental Toxicology and Pharmacology, 2009, 27:307-313.
    [14] BELLINGER D C. Very low lead exposures and children's neurodevelopment[J]. Current Opinion in Pediatrics, 2008, 20(2):172-177.
    [15] RICE C, GHORAI J K, ZALEWSKI K, et al. Developmental lead exposure causes startle response deficits in zebrafish[J]. Aquatic Toxicology, 2011, 105(3-4):600-608.
    [16] CHEN J, CHEN Y, LIU W, et al. Developmental lead acetate exposure induces embryonic toxicity and memory deficit in adult zebrafish[J]. Neurotoxicology and Teratology, 2012, 34(6):581-586.
    [17] ZHAO J, XU T, YIN D Q. Locomotor activity changes on zebrafish larvae with different 2,20,4,40-tetrabromodiphenyl ether (PBDE-47) embryonic exposure modes[J]. Chemosphere, 2014, 94:53-61.
    [18] WESTERFIELD M. A guide for the laboratory use of zebrafish (Danio rerio)[M]. Eugene, OR:University of Oregon Press, 2000.
    [19] 潘睿捷, 黄文平, 张斌, 等. 斑马鱼幼鱼运动行为测试评价饮用水安全[J]. 生态毒理学报, 2016, 11(4):18-25.

    PAN R J, HUANG W P, ZHANG B, et al. Toxicity assessment of drinking water using zebrafish swimming behavior tests[J]. Asian Journal of Ecotoxicology, 2016, 11(4):18-25(in Chinese).

    [20] ZHANG B, CHEN X L, PAN R J, et al. Effects of three different embryonic exposure modes of 2,2',4,4'-tetrabromodiphenyl ether on the path angle and social activity of zebra fish larvae[J]. Chemosphere, 2017, 169:542-549.
    [21] ZHU B R, WANG Q W, WANG X F, et al. Impact of co-exposure with lead and decabromodiphenyl ether(BDE-209) on thyroid function in zebrafish larvae[J]. Aquatic Toxicology, 2014, 157:186-195.
    [22] 张小晶. 胚胎期铅暴露对斑马鱼NMDA受体表达及行为的影响[D]. 温州:温州医学院, 2012. ZHANG X J. Effects on expression of NMDA receptor and behavior in zebrafish exposed to lead during embryonic period[D]. Wenzhou:Wenzhou Medical College, 2012(in Chinese).
    [23] ZHU B R, WANG Q W, SHI X J, et al. Effect of combined exposure to lead and decabromodiphenyl ether on neurodevelopment of zebrafish larvae[J]. Chemosphere, 2016, 144:1646-1654.
    [24] WIRBISKY S E, WEBER G J, LEE J W, et al. Novel dose-dependent alterations in excitatory GABA during embryonic development associated with lead (Pb) neurotoxicity[J]. Toxicology Letters, 2014, 229(1):1-8.
    [25] TU H W, FAN C J, CHEN X H, et al. Effects on cadmium, manganese, and lead on locomotor activity and neurexin 2A expression in zebrafish[J]. Environmental Toxicology and Chemistry, 2017, 38(8):2147-2154.
    [26] CHEN X J, HUANG C J, WANG X C, et al. BDE-47 disrupts axonal growth and motor behavior in developing zebrafish[J]. Aquatic Toxicology, 2012, 120-121:35-44.
    [27] CHEN L G, HUANG C J, HU C Y, et al. Acute exposure to DE-71:Effects on locomotor behavior and developmental neurotoxicity in zebrafish larvae[J]. Environmental Toxicology and Chemistry, 2012, 31(10):2338-2344.
    [28] CHEN L G, HUANG Y B, HUANG C J, et al. Acute exposure to DE-71 causes alterations in visual behavior in zebrafish larvae[J]. Environmental Toxicology and Chemistry, 2013, 32(6):1370-1375.
    [29] XU T, ZHAO J, Yin D Q, et al. High-throughput RNA sequencing reveals the effects of 2,2',4,4' -tetrabromodiphenyl ether on retina and bone development of zebrafish larvae[J]. BMC Genomics, 2015, 16(1):1-12.
    [30] ZHAO J, XU T, YIN D Q, et al. The regulatory roles of microRNA in effects of 2,2' 4,4'-tetrabromodiphenyl ether (BDE47) on the transcriptome of zebrafish larvae[J]. Plos One, 2017,12(1):e0169599.
    [31] XU T, LIU Y, PAN R J, et al. Vision, color vision, and visually guided behavior:The novel toxicological targets of 2,2',4,4'-tetrabromodiphenyl ether (BDE-47)[J]. Environmental Science & Technology Letters, 2017, 4(4):132-136.
    [32] VAN LEEUWEN J L, VOESENEK C J, MULLER U K. How body torque and Strouhal number change with swimming speed and developmental stage in larval zebrafish[J]. Journal of the Royal Society Interface, 2015, 12(110):1-11.
    [33] MULLER U K, VAN LEEUWEN J L. Swimming of larval zebrafish ontogeny of body waves and implications for locomotory development[J]. Journal of Experimental Biology, 2004, 207(5):853-868.
    [34] BUDICK S A, OMALLEY D M. Locomotor repertoire of the larval zebrafish swimming, turning and prey capture[J]. Journal of Experimental Biology, 2000, 203(17):2565-2579.
    [35] CRETON R. Automated analysis of behavior in zebrafish larvae[J]. Behavioural Brain Research, 2009, 203:127-136.
    [36] COLWILL R M, CRETON R. Imaging escape and avoidance behavior in zebrafish larvae[J]. Reviews in the Neurosciences, 2011, 22:63-73.
    [37] 邹苏琪, 殷梧, 杨昱鹏. 斑马鱼行为学实验在神经科学中的应用[J].生物化学与生物物理进展, 2009, 36(1):5-12.

    ZOU S Q, YIN W, YANG Y P, et al. The ethology application of zebrafish in neuroscience[J]. Progress in Biochemistry and Biophysics, 2009, 36(1):5-12(in Chinese).

    [38] BAILEY J M, OLIVERI A N, KARBHARI N, et al. Persistent behavioral effects following early life exposure to retinoic acid or valproic acid in zebrafish[J]. Neurotoxicology, 2016, 52:23-33.
  • 加载中
WeChat 点击查看大图
计量
  • 文章访问数:  1832
  • HTML全文浏览数:  1768
  • PDF下载数:  699
  • 施引文献:  0
出版历程
  • 收稿日期:  2017-08-09
  • 刊出日期:  2018-03-15
张晴, 张斌, 赵静, 白建峰, 尹大强. 环境相关浓度铅暴露诱导斑马鱼仔鱼神经行为毒性[J]. 环境化学, 2018, 37(3): 445-452. doi: 10.7524/j.issn.0254-6108.2017080905
引用本文: 张晴, 张斌, 赵静, 白建峰, 尹大强. 环境相关浓度铅暴露诱导斑马鱼仔鱼神经行为毒性[J]. 环境化学, 2018, 37(3): 445-452. doi: 10.7524/j.issn.0254-6108.2017080905
shu
ZHANG Qing, ZHANG Bin, ZHAO Jing, BAI Jianfeng, YIN Daqiang. Neurobehavioral toxicity of zebrafish larvae caused by lead exposure at environmentally relevant concentrations[J]. Environmental Chemistry, 2018, 37(3): 445-452. doi: 10.7524/j.issn.0254-6108.2017080905
Citation: ZHANG Qing, ZHANG Bin, ZHAO Jing, BAI Jianfeng, YIN Daqiang. Neurobehavioral toxicity of zebrafish larvae caused by lead exposure at environmentally relevant concentrations[J]. Environmental Chemistry, 2018, 37(3): 445-452. doi: 10.7524/j.issn.0254-6108.2017080905
shu

环境相关浓度铅暴露诱导斑马鱼仔鱼神经行为毒性

  • 1.  上海第二工业大学电子废弃物研究中心, 上海电子废弃物资源化协同创新中心, 上海, 201209;
  • 2.  同济大学环境科学与工程学院, 长江水环境教育部重点实验室, 上海, 200092
  • 收稿日期:  2017-08-09
基金项目:

国家自然科学基金（21507080，21577104），上海第二工业大学重点学科（XXKZD1602）和上海第二工业大学研究生项目基金（EGD16YJ046）资助.

摘要: 铅是公认的神经毒物，它的神经毒性是全世界备受关注的重要公共健康问题，特别是发育早期阶段铅暴露与神经行为变化之间的关联.斑马鱼被认为是研究发育早期阶段低浓度铅暴露诱导神经行为毒性的优良模式生物.因此，本研究以斑马鱼胚胎/仔鱼为研究对象，利用Zebrabox建立的行为学效应测试方法，包括运动行为、转动行为和社交行为，系统评价发育早期阶段环境相关浓度（以电子废弃物拆解场地为例）铅暴露的神经行为毒性效应.发育正常的斑马鱼胚胎暴露在浓度为0、5、10、20 μg·L-1铅暴露液中直至6 dpf.铅暴露对斑马鱼胚胎/仔鱼具有发育毒性作用，包括孵化率和存活率下降，卵黄囊肿、心包囊肿和脊柱弯曲等畸形现象.在光照条件下，铅暴露对斑马鱼仔鱼的运行行为和转向行为并未产生显著影响；但是在黑暗条件下，20 μg·L-1铅暴露后斑马鱼仔鱼运动距离和总转向次数均显著下降，但是其行进路径角度和偏转偏好却无变化.与运动行为和转向行为不同，在黑暗条件下，铅暴露对斑马鱼仔鱼的社交行为并未产生显著影响；但在光照条件下20 μg·L-1铅暴露却显著改变了仔鱼的社交次数.本研究结果表明，行为学指标不仅具备足够的灵敏度可用于指示电子废弃物拆解场地铅污染的潜在风险，还能更好地服务于人类健康早期预警的需求.

English Abstract

    全文HTML

参考文献 (38)

返回顶部

目录

/

返回文章
返回

Copyright © 2019 中国科学院生态环境研究中心