曝气量对微生物燃料电池脱氮的影响
Effect of aeration rate on nitrogen removal by microbial fuel cells
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摘要: 实验构建生物阴极双室微生物燃料电池，探究在微氧条件下曝气量对其产电性能和阴极脱氮的影响.以乙酸钠为碳源，氯化铵为氮源.实验在25℃温度下，阴极持续曝气，并控制反应器内为微氧状态，富集培养短程硝化反硝化菌群.实现了在特定曝气量条件下生物阴极短程硝化反硝化脱氮.实验结果表明，在曝气量为1.64 mL·min-1的条件下，短程硝化反硝化脱氮效果最好.亚硝态氮积累率为81.70%，总氮去除率达到69.66%，最大稳定电压达0.47 V左右，库伦效率为43.8%，产电效能较好.针对实际污水处理开展相关实验，MFC阴极短程硝化反硝化总氮去除率可达到81.93%，优于全程硝化反硝化.在短程硝化反硝化的微生物群落中，Betaproteobacteria纲和Thauera菌属在短程硝化反硝化中得到了有效的富集，有利于生物脱氮，并且Nitrosomonas菌是主要的氨氧化菌属.Abstract: A bio-cathode double chamber microbial fuel cell was constructed to investigate the influence of aeration rate on its electrical generation performance and cathodic nitrogen removal under micro-aerobic conditions. In the experiment, sodium acetate and ammonium chloride were used as carbon source and nitrogen source, respectively. The reactor was started at 25℃. Continuous aeration was applied to the cathode chamber for the micro-aerobic condition to enrich a short-cut nitrification and denitrification microbial community. The short-cut nitrification and denitrification were realized in the biological cathode at a specific aeration rate. The experimental results showed that at an aeration rate of 1.64 mL·min-1, short-cut nitrification and denitrification were the best. The accumulation rate of nitrite nitrogen was 81.70%, total nitrogen removal rate reached 69.66%, the maximum stable voltage was around 0.47 V, the coulombic efficiency was 43.8%, and the power generation was better. Related experiments on actual sewage treatment were carried out. The total nitrogen removal rate of short-cut nitrification and denitrification in MFC cathode reached 81.93%, which is better than that of the whole process nitrification and denitrification. In microbial community of short-cut nitrification and denitrification, Betaproteobacteria and Thauera were effectively enriched, which is good for biological nitrogen removal. Besides,Nitrosomonas is the main nitrite-oxidizing bacteria.
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