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董小娜, 陈泽慧, 毛林强, 王明新, 张文艺. 微生物溶藻进程与机制的三维荧光分析方法[J]. 环境化学, 2018, 37(6): 1337-1342. doi: 10.7524/j.issn.0254-6108.2017091206
引用本文: 董小娜, 陈泽慧, 毛林强, 王明新, 张文艺. 微生物溶藻进程与机制的三维荧光分析方法[J]. 环境化学, 2018, 37(6): 1337-1342. doi: 10.7524/j.issn.0254-6108.2017091206
shu
DONG Xiaona, CHEN Zehui, MAO Linqiang, WANG Mingxin, ZHANG Wenyi. Three-dimensional fluorescence analysis of the processes and mechanisms of algae-lysing by microorganism[J]. Environmental Chemistry, 2018, 37(6): 1337-1342. doi: 10.7524/j.issn.0254-6108.2017091206
Citation: DONG Xiaona, CHEN Zehui, MAO Linqiang, WANG Mingxin, ZHANG Wenyi. Three-dimensional fluorescence analysis of the processes and mechanisms of algae-lysing by microorganism[J]. Environmental Chemistry, 2018, 37(6): 1337-1342. doi: 10.7524/j.issn.0254-6108.2017091206
shu

微生物溶藻进程与机制的三维荧光分析方法

  • 1.

    常州大学环境与安全工程学院, 常州, 213164

  • 基金项目:

    国家自然科学基金（41571471）和江苏省及常州市科技项目（BE2016653，CE20175009，WS201621）资助.

Three-dimensional fluorescence analysis of the processes and mechanisms of algae-lysing by microorganism

  • 1.

    College of Environmental & Safety Engineering, Changzhou University, Changzhou, 213164, China

  • Fund Project: Supported by the National Natural Science Foundation of China(41571471)and Science and Technology Project of Jiangsu Province and Changzhou City(BE2016653, CE20175009, WS201621).

  • 摘要: 以铜绿微囊藻（Microcystis aeruginosa）为实验对象，利用三维荧光谱解析微生物溶藻进程与溶藻机制.构建了藻细胞数量-荧光光强、叶绿素a-荧光光强关系模型，验证荧光法测定藻液的准确性.通过Em 656 nm下荧光激发光谱，考察了溶藻菌R1菌（Lysinibacillus macroides）的溶藻能力.根据菌藻混合液三维荧光光谱图，解析了藻细胞分泌物和溶藻（降解）产物.结果表明，荧光光强可以用来表征藻细胞浓度，在低浓度下（浓度阈值为细胞数量120 cell·mL-1或chl-a含量0.2 mg·L-1），其与藻细胞及其叶绿素a呈正相关（R2>0.95，P<0.01）；采用荧光强度表征溶藻菌R1溶藻率，10 d溶藻率可达89.8%，与chl-a表征的溶藻率（82.64%）基本一致.菌藻混合液三维荧光光谱图解析表明，溶藻菌R1对铜绿微囊藻的溶藻产物中含有藻细胞溶解生成的可溶性物质、芳香族蛋白质；混合液中类腐殖酸大量减少，推测其为细菌分泌的溶藻活性物质.溶藻机理为细胞分泌胞外物质（酸性物质）作用于藻细胞→细胞结构被破坏→胞内物质（蛋白质物质等）释放出来.
    Abstract: Three-dimensional fluorescence spectroscopy(EEMs)was employed to study the processes and algae-lysing mechanisms by microorganism, with Microcystis aeruginosa as the experimental subject. The correlation of fluorescence intensity with the number of algal cell and chlorophyll-a were established, and used to examine the accuracy of algal cell concentration measured by EEMs. The algae-lysing ability of Lysinibacillus macroides was studied by fluorescence excitation spectroscopy at Em of 656 nm. The products of algae-lysing and secretion of bacteria were analyzed by identifying the mixtures of algae and bacteria with the help of EEMs. The results showed that the fluorescence intensity could be employed to measure the concentration of algae. When the number of algal cells was below 120 cell·mL-1 or quantity of chl-a was below 0.2 mg·L-1, the fluorescence intensity was linearly correlatied with the number of algal cells and quantity of chlorophyll-a, with a linear correlation coefficient of R2>0.95 and PMicrocystis aeruginosa involved the soluble algal cells secretions and aromatic proteins. Lysing of algae could be attributed to the presence of humic acids-like substances, because the amount of humic acids-like substances declined remarkably in the mixture of algae and bacteria. The proposed alge-lysing mechanism in that the bacteria cells secreted extracellular (acid-like) substances, which destroyed algae structure, and then the organelles in cells were released.
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  • 收稿日期:  2017-09-12
  • 刊出日期:  2018-06-15
董小娜, 陈泽慧, 毛林强, 王明新, 张文艺. 微生物溶藻进程与机制的三维荧光分析方法[J]. 环境化学, 2018, 37(6): 1337-1342. doi: 10.7524/j.issn.0254-6108.2017091206
引用本文: 董小娜, 陈泽慧, 毛林强, 王明新, 张文艺. 微生物溶藻进程与机制的三维荧光分析方法[J]. 环境化学, 2018, 37(6): 1337-1342. doi: 10.7524/j.issn.0254-6108.2017091206
shu
DONG Xiaona, CHEN Zehui, MAO Linqiang, WANG Mingxin, ZHANG Wenyi. Three-dimensional fluorescence analysis of the processes and mechanisms of algae-lysing by microorganism[J]. Environmental Chemistry, 2018, 37(6): 1337-1342. doi: 10.7524/j.issn.0254-6108.2017091206
Citation: DONG Xiaona, CHEN Zehui, MAO Linqiang, WANG Mingxin, ZHANG Wenyi. Three-dimensional fluorescence analysis of the processes and mechanisms of algae-lysing by microorganism[J]. Environmental Chemistry, 2018, 37(6): 1337-1342. doi: 10.7524/j.issn.0254-6108.2017091206
shu

微生物溶藻进程与机制的三维荧光分析方法

  • 1. 常州大学环境与安全工程学院, 常州, 213164
  • 收稿日期:  2017-09-12
基金项目:

国家自然科学基金（41571471）和江苏省及常州市科技项目（BE2016653，CE20175009，WS201621）资助.

摘要: 以铜绿微囊藻（Microcystis aeruginosa）为实验对象，利用三维荧光谱解析微生物溶藻进程与溶藻机制.构建了藻细胞数量-荧光光强、叶绿素a-荧光光强关系模型，验证荧光法测定藻液的准确性.通过Em 656 nm下荧光激发光谱，考察了溶藻菌R1菌（Lysinibacillus macroides）的溶藻能力.根据菌藻混合液三维荧光光谱图，解析了藻细胞分泌物和溶藻（降解）产物.结果表明，荧光光强可以用来表征藻细胞浓度，在低浓度下（浓度阈值为细胞数量120 cell·mL-1或chl-a含量0.2 mg·L-1），其与藻细胞及其叶绿素a呈正相关（R2>0.95，P<0.01）；采用荧光强度表征溶藻菌R1溶藻率，10 d溶藻率可达89.8%，与chl-a表征的溶藻率（82.64%）基本一致.菌藻混合液三维荧光光谱图解析表明，溶藻菌R1对铜绿微囊藻的溶藻产物中含有藻细胞溶解生成的可溶性物质、芳香族蛋白质；混合液中类腐殖酸大量减少，推测其为细菌分泌的溶藻活性物质.溶藻机理为细胞分泌胞外物质（酸性物质）作用于藻细胞→细胞结构被破坏→胞内物质（蛋白质物质等）释放出来.

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