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A/SMBBR处理生活污水效能研究及生物膜菌群结构分析

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贾晓硕, 谢者行, 李卫平, 杨文焕, 敬双怡, 刘文加. A/SMBBR处理生活污水效能研究及生物膜菌群结构分析[J]. 环境化学, 2018, 37(12): 2651-2658. doi: 10.7524/j.issn.0254-6108.2018010501
引用本文: 贾晓硕, 谢者行, 李卫平, 杨文焕, 敬双怡, 刘文加. A/SMBBR处理生活污水效能研究及生物膜菌群结构分析[J]. 环境化学, 2018, 37(12): 2651-2658. doi: 10.7524/j.issn.0254-6108.2018010501
shu
JIA Xiaoshuo, XIE Zhexing, LI Weiping, YANG Wenhuan, JING Shuangyi, LIU Wenjia. A/SMBBR treatment effectiveness of domestic sewage research and structural analysis of biofilm flora[J]. Environmental Chemistry, 2018, 37(12): 2651-2658. doi: 10.7524/j.issn.0254-6108.2018010501
Citation: JIA Xiaoshuo, XIE Zhexing, LI Weiping, YANG Wenhuan, JING Shuangyi, LIU Wenjia. A/SMBBR treatment effectiveness of domestic sewage research and structural analysis of biofilm flora[J]. Environmental Chemistry, 2018, 37(12): 2651-2658. doi: 10.7524/j.issn.0254-6108.2018010501
shu

A/SMBBR处理生活污水效能研究及生物膜菌群结构分析

  • 1.

    内蒙古科技大学能源与环境学院, 包头, 014010;

  • 2.

    北汽特来电(北京)新能源科技有限公司, 北京, 100176

  • 基金项目:

    内蒙古高校项目（NJZY16164）和内蒙古科技大学产学研项目（PY201501）资助.

A/SMBBR treatment effectiveness of domestic sewage research and structural analysis of biofilm flora

  • 1.

    School of Energy and E nvironment, Inner Mongolia University of Science and Technology, Baotou, 014010, China;

  • 2.

    Beijing AUV Bus Branch Company of Beiqi Foton Motor Co., Ltd., Beijing, 100176, China

  • Fund Project: Supported by Inner Mongolia University Project (NJZY16164) and the Production and Research Project of Inner Mongolia University of Science and Technology(PY201501).

  • 摘要: 采用SDC-03作为生物载体，考察特异性移动床生物膜法对生活污水中有机污染物的去除效果，并运用Illumina Hiseq高通量测序技术对微生物进行分析.实验结果表明，COD、氨氮和总氮的平均出水浓度分别为25.65 mg·L-1、5.14 mg·L-1、14.55 mg·L-1，平均去除率分别为91.05%、92.39%、83.44%.该工艺能适应较低温度，最佳HRT和DO浓度分别为10-12 h、3-4 mg·L-1之间.采用Chao1指数、Shannon指数、Simpson指数及PD whole tree值等指数对微生物种群多样性进行分析.对反应器的微生物从门和属两个科目进行分析，得出门水平丰度前10的微生种和属的物种组成.门水平前十的物种分别为变形菌门（Proteobacteria）、拟杆菌门（Bacteroidetes）、芽单胞菌门（Gemmatimonadetes）、Saccharibacteria、厚壁菌门（Firmicutes）、绿弯菌门（Chlorofflexi）、放线菌门（Actinobacteria）、硝化螺旋菌门（Nitrospira）、酸杆菌门（Acidobacteria）、Thermomicrobia.变形菌门和拟杆菌门大量存在于在好氧池中，芽单胞菌门和厚壁菌门有助于反硝化反应的进行；在属水平中得知未知的菌属占大部分.
    Abstract: Using SDC-03 as the biological carrier, this work examined the removal efficiency of the organic pollutants in domestic sewage by A/SMBBR treatment and analyzed the microorganisms therein by the Illumina Hiseq high-throughput sequencing technology. The experiments showed demonstrated that the average effluent concentrations of COD, ammonia nitrogen and total nitrogen were respectively 25.65 mg·L-1, 5.14 mg·L-1 and 14.55 mg·L-1, with average removal ratios of 91.05%, 92.39% and 83.44%. It was demonstrated that this process could better accommodate low temperatures. The most favorable hydraulic retention time remained between 10 and 12 hours, and the optimum dissolved oxygen concentration stayed between 3 and 4 mg·L-1. The microbial diversity was analyzed through Chao1 index, Shannon index, Simpson index, and PD whole tree methods. Microorganisms in the reactor were observed at both the phylum and the genus level. Based on the abundance level, the top 10 species of both phyluon and genus were derived. At the phylum level, Proteobacteria, Bacteroidetes, Gemmat imonadetes, Saccharibacteria, Firmicutes, Chlorofflexi, Actinobacteria, Nitrospira, Acidobacte-ria and Thermomicrobia were among the top 10. A large amount of Proteobacteria and Bacteroidetes were observed in aerobic tanks and Gemmatimonadetes and thick-walled fungi proved to be helpful for denitrification. At the genus level, unknown bacteria accounted for the most.
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  • 收稿日期:  2018-01-05
  • 刊出日期:  2018-12-15
贾晓硕, 谢者行, 李卫平, 杨文焕, 敬双怡, 刘文加. A/SMBBR处理生活污水效能研究及生物膜菌群结构分析[J]. 环境化学, 2018, 37(12): 2651-2658. doi: 10.7524/j.issn.0254-6108.2018010501
引用本文: 贾晓硕, 谢者行, 李卫平, 杨文焕, 敬双怡, 刘文加. A/SMBBR处理生活污水效能研究及生物膜菌群结构分析[J]. 环境化学, 2018, 37(12): 2651-2658. doi: 10.7524/j.issn.0254-6108.2018010501
shu
JIA Xiaoshuo, XIE Zhexing, LI Weiping, YANG Wenhuan, JING Shuangyi, LIU Wenjia. A/SMBBR treatment effectiveness of domestic sewage research and structural analysis of biofilm flora[J]. Environmental Chemistry, 2018, 37(12): 2651-2658. doi: 10.7524/j.issn.0254-6108.2018010501
Citation: JIA Xiaoshuo, XIE Zhexing, LI Weiping, YANG Wenhuan, JING Shuangyi, LIU Wenjia. A/SMBBR treatment effectiveness of domestic sewage research and structural analysis of biofilm flora[J]. Environmental Chemistry, 2018, 37(12): 2651-2658. doi: 10.7524/j.issn.0254-6108.2018010501
shu

A/SMBBR处理生活污水效能研究及生物膜菌群结构分析

  • 1.  内蒙古科技大学能源与环境学院, 包头, 014010;
  • 2.  北汽特来电(北京)新能源科技有限公司, 北京, 100176
  • 收稿日期:  2018-01-05
基金项目:

内蒙古高校项目（NJZY16164）和内蒙古科技大学产学研项目（PY201501）资助.

摘要: 采用SDC-03作为生物载体，考察特异性移动床生物膜法对生活污水中有机污染物的去除效果，并运用Illumina Hiseq高通量测序技术对微生物进行分析.实验结果表明，COD、氨氮和总氮的平均出水浓度分别为25.65 mg·L-1、5.14 mg·L-1、14.55 mg·L-1，平均去除率分别为91.05%、92.39%、83.44%.该工艺能适应较低温度，最佳HRT和DO浓度分别为10-12 h、3-4 mg·L-1之间.采用Chao1指数、Shannon指数、Simpson指数及PD whole tree值等指数对微生物种群多样性进行分析.对反应器的微生物从门和属两个科目进行分析，得出门水平丰度前10的微生种和属的物种组成.门水平前十的物种分别为变形菌门（Proteobacteria）、拟杆菌门（Bacteroidetes）、芽单胞菌门（Gemmatimonadetes）、Saccharibacteria、厚壁菌门（Firmicutes）、绿弯菌门（Chlorofflexi）、放线菌门（Actinobacteria）、硝化螺旋菌门（Nitrospira）、酸杆菌门（Acidobacteria）、Thermomicrobia.变形菌门和拟杆菌门大量存在于在好氧池中，芽单胞菌门和厚壁菌门有助于反硝化反应的进行；在属水平中得知未知的菌属占大部分.

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