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罗梦, 易皓, 陈静, 张文哲, 韩云平, 肖本益. 高效液相串联质谱法检定活性污泥中N-高丝氨酸内酯[J]. 环境化学, 2019, 38(2): 281-286. doi: 10.7524/j.issn.0254-6108.2018030704
引用本文: 罗梦, 易皓, 陈静, 张文哲, 韩云平, 肖本益. 高效液相串联质谱法检定活性污泥中N-高丝氨酸内酯[J]. 环境化学, 2019, 38(2): 281-286. doi: 10.7524/j.issn.0254-6108.2018030704
shu
LUO Meng, YI Hao, CHEN Jing, ZHANG Wenzhe, HAN Yunping, XIAO Benyi. Determination of N-acyl-homoserine lactones in activated sludge by high performance liquid chromatography-tandem mass spectrometry[J]. Environmental Chemistry, 2019, 38(2): 281-286. doi: 10.7524/j.issn.0254-6108.2018030704
Citation: LUO Meng, YI Hao, CHEN Jing, ZHANG Wenzhe, HAN Yunping, XIAO Benyi. Determination of N-acyl-homoserine lactones in activated sludge by high performance liquid chromatography-tandem mass spectrometry[J]. Environmental Chemistry, 2019, 38(2): 281-286. doi: 10.7524/j.issn.0254-6108.2018030704
shu

高效液相串联质谱法检定活性污泥中N-高丝氨酸内酯

  • 1.

    中国科学院生态环境研究中心, 北京, 100085;

  • 2.

    中国科学院大学, 北京, 100049;

  • 3.

    环境保护部华南环境科学研究所, 广州, 510655;

  • 4.

    西安建筑科技大学环境与市政工程学院, 西安, 710055

  • 基金项目:

    国家自然科学基金（51378492）和国家水体污染控制与治理科技重大专项（2017ZX07106003）资助

Determination of N-acyl-homoserine lactones in activated sludge by high performance liquid chromatography-tandem mass spectrometry

  • 1.

    Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing, 100085, China;

  • 2.

    University of Chinese Academy of Sciences, Beijing, 100049, China;

  • 3.

    South China Institute of Environmental Sciences, Ministry of Environmental Protection, Guangzhou, 510655, China;

  • 4.

    School of Environmental and Municipal Engineering, Xi'an University of Architecture and Technology, Xi'an, 710055, China

  • Fund Project: Supported by the National Natural Science Foundation of China(51378492)and the Major Science and Technology Program for Water Pollution Control and Treatment of China (2017ZX07106003)

  • 摘要: N-高丝氨酸内酯（AHLs）是革兰氏阴性菌群体感应系统中最重要的一类信号分子.本研究建立了高效液相串联质谱（HPLC-MS/MS）检测活性污泥中7种AHLs的方法.采用乙腈-水为流动相，选择电喷雾源正离子模式，经过梯度洗脱分离，在多反应监测模式下进行测定，分析时间为10 min.本方法在1—1000 μg·L-1范围内具有良好的线性关系，仪器检出限为1.0—2.0 μg·L-1，仪器定量限为2.0—5.0 μg·L-1，平均加标回收率在80.0%—113.4%之间.采用该方法对某污水处理厂和实验室序批式活性污泥装置（SBR）中活性污泥进行检测，均可检测到不同种类的AHLs，并具有良好的峰型及分离效果，说明方法可满足实际测定要求.
    Abstract: N-homoserine lactones (AHLs) are vital quorum-sensing signal molecules of gram-negative bacteria. A high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) method was established to determine the AHLs in activated sludge. A mixture of acetonitrile and water served as the mobile phase and an electrospray positive ion mode was set. After seperation by mobile phase with gradient elution, the sample was measured in multi-reaction monitoring mode with a detection time of 10 min. The results showed that the method had a good linearity in the range of 1-1000 μg·L-1. The instrument detection limit, instrument quantification limit and average recovery efficiency were 1.0-2.0 μg·L-1, 2.0-5.0 μg·L-1, and 80.0%-113.4%, respectively. When this method was used to analyze the activated sludge from wastewater treatment plant and lab-scale sequential batch activated sludge reactor (SBR) system, different types of AHLs were detected with a good peak shapes and separation efficiency. Therefore, this method can satisfy the actual requirement for the measurement of AHLs in activated sludge.
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  • 收稿日期:  2018-03-07
  • 刊出日期:  2019-02-15
罗梦, 易皓, 陈静, 张文哲, 韩云平, 肖本益. 高效液相串联质谱法检定活性污泥中N-高丝氨酸内酯[J]. 环境化学, 2019, 38(2): 281-286. doi: 10.7524/j.issn.0254-6108.2018030704
引用本文: 罗梦, 易皓, 陈静, 张文哲, 韩云平, 肖本益. 高效液相串联质谱法检定活性污泥中N-高丝氨酸内酯[J]. 环境化学, 2019, 38(2): 281-286. doi: 10.7524/j.issn.0254-6108.2018030704
shu
LUO Meng, YI Hao, CHEN Jing, ZHANG Wenzhe, HAN Yunping, XIAO Benyi. Determination of N-acyl-homoserine lactones in activated sludge by high performance liquid chromatography-tandem mass spectrometry[J]. Environmental Chemistry, 2019, 38(2): 281-286. doi: 10.7524/j.issn.0254-6108.2018030704
Citation: LUO Meng, YI Hao, CHEN Jing, ZHANG Wenzhe, HAN Yunping, XIAO Benyi. Determination of N-acyl-homoserine lactones in activated sludge by high performance liquid chromatography-tandem mass spectrometry[J]. Environmental Chemistry, 2019, 38(2): 281-286. doi: 10.7524/j.issn.0254-6108.2018030704
shu

高效液相串联质谱法检定活性污泥中N-高丝氨酸内酯

  • 1.  中国科学院生态环境研究中心, 北京, 100085;
  • 2.  中国科学院大学, 北京, 100049;
  • 3.  环境保护部华南环境科学研究所, 广州, 510655;
  • 4.  西安建筑科技大学环境与市政工程学院, 西安, 710055
  • 收稿日期:  2018-03-07
基金项目:

国家自然科学基金（51378492）和国家水体污染控制与治理科技重大专项（2017ZX07106003）资助

摘要: N-高丝氨酸内酯（AHLs）是革兰氏阴性菌群体感应系统中最重要的一类信号分子.本研究建立了高效液相串联质谱（HPLC-MS/MS）检测活性污泥中7种AHLs的方法.采用乙腈-水为流动相，选择电喷雾源正离子模式，经过梯度洗脱分离，在多反应监测模式下进行测定，分析时间为10 min.本方法在1—1000 μg·L-1范围内具有良好的线性关系，仪器检出限为1.0—2.0 μg·L-1，仪器定量限为2.0—5.0 μg·L-1，平均加标回收率在80.0%—113.4%之间.采用该方法对某污水处理厂和实验室序批式活性污泥装置（SBR）中活性污泥进行检测，均可检测到不同种类的AHLs，并具有良好的峰型及分离效果，说明方法可满足实际测定要求.

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