黄孢原毛平革菌对氯代蒽的生物降解及降解途径
Biodegradation of chlorinated anthracene by Phanerochaete chrysosporium and its degradation pathway
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摘要: 氯代多环芳烃（chlorinated polycyclic aromatic hydrocarbons，Cl-PAHs）是多环芳烃的氯代衍生物，其毒性与母体相当甚至高于母体，在各种环境介质中广泛存在且难以降解，对生态环境和人类健康具有一定的潜在威胁.微生物降解是环境中去除有机物的主要途径之一，本文以白腐真菌的模式菌种-黄孢原毛平革菌（Phanerochaete chrysosporium，Pc）为代表，优化了Pc菌对氯代蒽的降解条件、探究了降解效果以及降解动力学，并分析了可能的降解途径.结果表明，Pc菌对氯代蒽有一定的降解能力.当液体培养基的初始pH值为4.5，Pc菌接种量约为每毫升1×105个时，在35℃，120 r·min-1的恒温摇床中培养6 d后，接入浓度为100 mg·L-1的底物能够达到较高的降解效率.在此条件下降解16 d后9-ClAnt和9，10-Cl2Ant的降解率分别达到了96.45%和92.83%.动力学分析表明，Pc菌降解氯代蒽的过程符合一级动力学方程.分析降解过程，检测到5种降解中间产物，结合生物催化反应的特点推测了氯代蒽可能的降解途径.Abstract: Chlorinated polycyclic aromatic hydrocarbons (Cl-PAHs) are chlorinated derivatives of PAHs, but the toxicity of them is equivalent or even higher than that of parent PAHs, Cl-PAHs are widely present in the environments and difficult to be degraded, posing a potential threat to the ecological environment and human health. Microbial degradation has been suggested as one of the best ways to remove organic pollutants from polluted environments. In this study, the optimized degradation conditions, biodegradation ability, the degradation kinetics of chlorinated anthracene(Cl-Ant) as well as the possible degradation pathway were investigated by the model species-Phanerochaete chrysosporium, The results showed a good effect of degrading Cl-Ant under the initial liquid medium at pH 4.5, 1×105 cells·mL-1 Phanerochaete chrysosporium at 35℃, 120 r·min-1 incubation for 6 days,after then 100 mg·L-1 substrate inoculated. After 16 days' experiments, the degradation efficiency of 9-ClAnt and 9,10-Cl2Ant reached to 96.45% and 92.83%, respectively. The kinetic analysis of Cl-Ant degradation follows the rule of the first-order kinetic equation. Five types of intermediate products were detected from the degradation system. Combining with the characteristics of biocatalytic reaction, the possible degradation pathways of Cl-Ant were proposed in this research.
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