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一株高效邻苯二甲酸二丁酯降解菌的筛选、鉴定及其降解特性研究

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李容榛, 李成, 赵暹, 刘春敬, 孟靖凯, 谢建治. 一株高效邻苯二甲酸二丁酯降解菌的筛选、鉴定及其降解特性研究[J]. 环境化学, 2019, (10): 2274-2282. doi: 10.7524/j.issn.0254-6108.2018111502
引用本文: 李容榛, 李成, 赵暹, 刘春敬, 孟靖凯, 谢建治. 一株高效邻苯二甲酸二丁酯降解菌的筛选、鉴定及其降解特性研究[J]. 环境化学, 2019, (10): 2274-2282. doi: 10.7524/j.issn.0254-6108.2018111502
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LI Rongzhen, LI Cheng, ZHAO Xian, LIU Chunjing, MENG Jinkai, XIE Jian. Isolation and identification of a highly efficient DBP degrading bacteria and its degradation characteristics[J]. Environmental Chemistry, 2019, (10): 2274-2282. doi: 10.7524/j.issn.0254-6108.2018111502
Citation: LI Rongzhen, LI Cheng, ZHAO Xian, LIU Chunjing, MENG Jinkai, XIE Jian. Isolation and identification of a highly efficient DBP degrading bacteria and its degradation characteristics[J]. Environmental Chemistry, 2019, (10): 2274-2282. doi: 10.7524/j.issn.0254-6108.2018111502
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一株高效邻苯二甲酸二丁酯降解菌的筛选、鉴定及其降解特性研究

  • 河北农业大学资源与环境科学学院, 河北省农田生态环境重点实验室, 保定, 071001

    • 通讯作者: 谢建治, E-mail: xjianzhi@126.com
  • 基金项目:

    国家水体污染控制与治理科技重大专项（2015ZX07203-005）和河北省高等学校科学技术研究青年基金（QN2016255）资助.

Isolation and identification of a highly efficient DBP degrading bacteria and its degradation characteristics

  • Key Laboratory of Ecological Environment of FarmLand in Hebei Province, College of Resources and Environmental Science, Agricultural University of Hebei, Baoding, 071001, China

    • Corresponding author: XIE Jian, xjianzhi@126.com
  • Fund Project: Supported by the National Water Pollution Control and Management Technology Major Project of China(2015ZX07203-005) and the Science and Technology Research Fund for Young Scholars of Hebei Higher Institutions(QN2016255).

  • 摘要: 从活性污泥中分离出1株以邻苯二甲酸二丁酯（DBP）为碳源和能源生长的高效降解菌DP-2，经形态观察、生化鉴定及16S rDNA序列分析，鉴定该菌株为不动杆菌（Acinetobacter sp.）.采用单因素试验研究了不同试验条件（接种量、DBP浓度、NaCl浓度和碳源）对菌株DBP降解特性的影响，结果表明：接种量大于10%时，菌株DP-2在3 d内对初始浓度为10 mg·L-1的DBP降解率可达到90%以上；DBP初始浓度为5—50 mg·L-1时，菌株在6 d内对DBP降解率均能达到90%以上，但高浓度DBP会影响菌株DP-2生长，DBP浓度为1000 mg·L-1时，DBP降解率仅为26.88%；菌株降解DBP的最佳NaCl浓度范围为0—20 g·L-1；此外，醋酸钠、蔗糖、葡萄糖添加对于菌株降解DBP均有一定的促进作用，其中葡萄糖效果最为明显.在此基础上，采用响应曲面法优化了菌株降解DBP的培养条件并进行了试验验证，在盐度为5 g·L-1，接种量为17.14%，底物浓度为9.81 mg·L-1，菌株对DBP的降解率为85.86%.
    Abstract: A bacterial strain DP-2, which could utilize dibutyl phthalate (DBP) as the carbon source and energy source, was isolated from activated sludge of sewage treatment plant. This strain was identified as Acinetobacter sp. by the combination of morphological observation, biochemical identification and 16S rDNA sequence analysis. The effect of inoculation amount, DBP concentration, NaCl concentration and carbon source on the DBP degradation characteristics of DP-2 was studied by a single factor test. The results showed that the DBP degradation rate of DP-2 could reach more than 90% within 3 days when the initial inoculation amount was more than 10% and the initial DBP concentration was 10 mg·L-1. The degradation rate of DBP could reach more than 90% within 6 days when the initial DBP concentration was 5-50 mg·L-1, however, the DBP degradation rate was only 26.88% when the DBP concentration reached to 1000 mg·L-1, which indicated higher DBP concentration could impact the growth of DP-2. The optimum NaCl concentration range of DP-2 was 0-20 g·L-1 for DBP degradation. In addition, sodium acetate, sucrose and glucose could promote the degradation of DBP, and the effect of glucose was the most obvious. Based on this, a response surface methodology test was conducted to optimize the culture conditions of DP-2 for DBP degradation. The results showed that the DBP degradation rate of DP-2 was 85.86% at salinity of 5 g·L-1, inoculation amount of 17.14% and DBP concentration of 9.81 mg·L-1.
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  • 收稿日期:  2018-11-15

一株高效邻苯二甲酸二丁酯降解菌的筛选、鉴定及其降解特性研究

    通讯作者: 谢建治, E-mail: xjianzhi@126.com
  • 河北农业大学资源与环境科学学院, 河北省农田生态环境重点实验室, 保定, 071001
  • 收稿日期:  2018-11-15
基金项目:

国家水体污染控制与治理科技重大专项（2015ZX07203-005）和河北省高等学校科学技术研究青年基金（QN2016255）资助.

摘要: 从活性污泥中分离出1株以邻苯二甲酸二丁酯（DBP）为碳源和能源生长的高效降解菌DP-2，经形态观察、生化鉴定及16S rDNA序列分析，鉴定该菌株为不动杆菌（Acinetobacter sp.）.采用单因素试验研究了不同试验条件（接种量、DBP浓度、NaCl浓度和碳源）对菌株DBP降解特性的影响，结果表明：接种量大于10%时，菌株DP-2在3 d内对初始浓度为10 mg·L-1的DBP降解率可达到90%以上；DBP初始浓度为5—50 mg·L-1时，菌株在6 d内对DBP降解率均能达到90%以上，但高浓度DBP会影响菌株DP-2生长，DBP浓度为1000 mg·L-1时，DBP降解率仅为26.88%；菌株降解DBP的最佳NaCl浓度范围为0—20 g·L-1；此外，醋酸钠、蔗糖、葡萄糖添加对于菌株降解DBP均有一定的促进作用，其中葡萄糖效果最为明显.在此基础上，采用响应曲面法优化了菌株降解DBP的培养条件并进行了试验验证，在盐度为5 g·L-1，接种量为17.14%，底物浓度为9.81 mg·L-1，菌株对DBP的降解率为85.86%.

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