大气主要矿物颗粒/金黄色葡萄球菌模拟复合体特征分析
Characteristic analysis of atmospheric major mineral particles/ staphylococcus aureus simulation complex
-
摘要: 大气环境污染日益复杂，大气中矿物颗粒与人体表面正常菌的复合体性质现在还不清楚，是否对人体具有危害还不得而知，大气主要矿物颗粒/金黄色葡萄球菌复合体特征的研究已经迫在眉睫.本文选取了3种矿物颗粒（石英、方解石、蒙脱石）和金黄色葡萄球菌，在成功制备矿物颗粒/金黄色葡萄球菌复合体的基础上，结合粒径分析仪、扫描电镜（SEM）、Zeta电位分析仪、傅里叶红外光谱仪（FTIR）等技术，对所制备的矿物/细菌复合体的粒径、形貌、表面电性及表面基团特征进行分析.粒径结果表明形成后的矿物/细菌复合体呈现粒径增大趋势；SEM结果表明，大量的金黄色葡萄球菌及其代谢产物粘附在矿物颗粒絮凝团表面；Zeta电位结果显示，复合体的电位值趋于矿物或者细菌的电位值；FTIR结果显示，矿物颗粒/金黄色葡萄球菌复合体中出现了来自金黄色葡萄球菌的特征峰，矿物颗粒其他基团出现了位移的现象.Abstract: Atmospheric environmental pollution is becoming more and more intricacy, the nature and hazard of complexes between mineral particles in the atmosphere and normal bacteria on the surface of the human body is still unclear, therefore, the characteristic research of major atmospheric mineral particles/Staphylococcus aureus complexes is imminent. In this paper, Three mineral particles (quartz, calcite and montmorillonite) and Staphylococcus aureus was selected to preparation complexes. On this basis, the particle size, morphology, surface electrical properties and surface group characteristics of mineral granule/Staphylococcus aureus complexes were analyzed through particle size analyzer, scanning electron microscope (SEM), Zeta potential analyzer and Fourier infrared spectrometer (FTIR). The following results were concluded. The particle size results showed that the particle size of mineral/bacterial complexes was bigger than the orginal mineral. Secondly, scanning electron microscope results showed that a large number of Staphylococcus aureus and its metabolites adhered to the surface of mineral floc. And then Zeta potential results showed that the potential value of the complex tends to the mineral or bacteria potential value. Finally, FTIR results showed that the characteristic peaks from Staphylococcus aureus appeared in mineral granules/Staphylococcus aureus complex, and the displacement phenomenon occurs in other groups of mineral particles. This study is expected to provide basic data support for solving the complex pollution problem in the atmospheric environment with practical significance.
-
-
[1] WANG G H, HUANG L M, GAO S X, et al. Measurements of PM10 and PM2.5 in urban area of Nanjing, China and the assessment of pulmonary deposition of particle mass[J]. Chemosphere, 2002, 48(7):689-695. [2] INBO O, JIHO L, KANGMO A,et al. Association between particulate matter concentration and symptoms of atopic dermatitis in children living in an industrial urban area of South Korea[J]. Environmental Research, 2018, 160:462-468. [3] 杨继雷, 姚秀叶, 袁晓飞, 等. 张家口市大气颗粒物PM2.5、PM10浓度与慢性阻塞性肺疾病住院人次的关系研究[J]. 现代生物医学进展, 2018, 6:1167-1171,1200. YANG J L, YAO X Y, YUAN X F, et al. Relationship between concentration of airborne particulate matter PM2.5 and PM10 and number of inpatients with chronic obstructive pulmonary disease in Zhangjiakou City[J]. Progress in Modern Biomedicine, 2018, 6:1167-1171,1200(in Chinese).
[4] 孙兆彬,安兴琴,崔甍甍,等. 北京地区颗粒物健康效应研究——沙尘天气、非沙尘天气下颗粒物(PM2.5、PM10)对心血管疾病入院人次的影响[J]. 中国环境科学, 2016, 36(8):2536-2544. SUN Z B, AN X Q, CUI M M, et al. The effect of PM2.5 and PM10 on cardiovascular and cerebrovascular diseases admission visitors in Beijing areas during dust weather, non-dust weather and haze pollution[J]. China Environmental Science, 2016, 36(8):2536-2544(in Chinese).
[5] 白子娜, 段争. 石家庄市大气颗粒物(PM10/PM2.5)对下呼吸道感染住院影响的病例交叉研究[J]. 解放军医学杂志, 2016, 41(2):123-129. BAI Z L, DUAN Z. Atmospheric particulate matter and hospital admission due to lower respiratory tract infection:A case-cross study in Shijiazhuang, China[J]. Medical Journal of Chinese People's Liberation Army, 2016, 41(2):123-129(in Chinese).
[6] 胡敏, 唐倩, 彭剑飞, 等. 我国大气颗粒物来源及特征分析[J]. 环境与可持续发展, 2011, 36(5):15-19. HU M, TANG Q, PENG J F, et al. Study on characterization and source apportionment of atmospheric particulate matter in China[J]. Environment and Sustainable Development, 2011, 36(5):15-19(in Chinese).
[7] MANSOUR A, ALGHAMDI, MAGDY S, MARIA A R, et al. Microorganisms associated particulate matter:A preliminary study[J]. Science of the Total Environment,2014,479-480:109-116. [8] 清华研究组:北京雾霾藏1300种微生物[J]. 化学分析计量, 2014,23(2):53. Tsinghua research group:Beijing smog conceal 1300 kinds of microorganism[J]. Chemical Analysis and Meterage, 2014,23(2):53.
[9] HARA K, ZHANG D Z. Bacterial abundance and viability in long-range transported dust[J]. Atmospheric Environment,2012,47:20-25. [10] 董发勤, 代群威, 贺小春, 等. 可吸入矿物细颗粒物与微生物的相互作用[J]. 岩石矿物学杂质, 2009, 28(6):611-616. DONG F Q, DAI Q W, HE X C, et al. The interaction between inhalable mineral granules and microorganisms[J]. Acta Petrologica ET Mineralogica, 2009, 28(6):611-616(in Chinese).
[11] MUELLER B. Experimental interactions between clay minerals and bacteria:A review[J]. Pedosphere, 2015, 25(6):799-810. [12] DU H H, CHEN W L, CAI P, et al. Cd(Ⅱ) sorption on montmorillonite-humic acid-bacteria composites[J]. Scientific Reports, 2016, 6:194-199. [13] HUANG Q Y, WU H Y, CAI P, et al. Atomic force microscopy measurements of bacterial adhesion and biofilm formation onto clay-sized particles[J]. Scientific Reports, 2015, 5:16857-16869. [14] THEWES N, LOSKILL P, SPENGLER C, et al. A detailed guideline for the fabrication of single bacterial probes used for atomic force spectroscopy[J]. The European Physical Journal E, 2015, 38:140-149. [15] 张东晨, 王涛, 侯志翔, 等. 矿物表面性质生物调节机理的研究[J]. 洁净煤技术, 2013, 19(1):5-9 ,23. ZHANG D C, WANG T, HOU Z,X, et al.Mechanism of microorganism regulating mineral surface properties[J]. Clean Coal Technology, 2013, 19(1):5-9, 23(in Chinese).
[16] 陈红霞. 蒙脱石的吸附特征及其在环境治理中的应用[J]. 化工管理, 2018(30):138-139. CHEN H X. Adsorption characteristics of montmorillonite and its application in environmental treatment[J]. Chemical Enterprise Management, 2018 (30):138-139(in Chinese).
[17] [18] 乐毅全, 王土芬. 环境工程微生物[M]. 北京:化学工业出版社, 2011. LE Y Q, WANG T F. Environmental engineering microorganism[M]. Beijing:Chemical Industry Press,2011(in Chinese). [19] 赵玉连, 代群威, 董发勤, 等. 短小芽孢杆菌-蒙脱石相互作用实验研究[J]. 岩石矿物学杂志, 2015, 34(6):939-944. ZHAO Y L, DAI Q W, DONG F Q, et al. An experimental study of the interaction between Bacillus pumilus and montmorillonite[J]. Acta Petrologica ET Mineralogica, 2015, 34(6):939-944(in Chinese).
[20] 荣兴民, 黄巧云, 陈雯莉, 等. 土壤矿物与微生物相互作用的机理及其环境效应[J]. 生态学报, 2008, 28(1):376-387. RONG X M, HUANG Q Y, CHEN W L, et al. Interaction mechanisms of soil minerals with microorganisms and their environmental significance[J]. Acta Ecologica Sinica, 2008, 28(1):376-387(in Chinese).
[21] 王利明, 董发勤, 李圃, 等. 矿物粉尘与人体3种正常细菌相互作用的机制研究[J]. 国际检验医学杂志, 2006, 27(6):481-483. WANG L M, DONG F Q, LI P, et al. Study on the effect of mineral dusts on three kinds of natural normal bacterials in vitro[J]. International Journal of Laboratory Medicine, 2006, 27(6):481-483(in Chinese).
[22] 向红, 周藜, 廖春, 等. 金黄色葡萄球菌及其引起的食物中毒的研究进展[J]. 中国食品卫生杂志, 2015, 27(2):196-199. XIANG H, ZHOU L, LIAO C, et al. Progress of staphylococcus aureus and food poisoning[J]. Chinese Journal of Food Hygiene, 2015, 27(2):196-199(in Chinese).
[23] 高正旭,王晓玲,向华,等. 武汉市2014-2017年大气污染物分布特征及其潜在来源分析[J]. 环境科学学报, 2018, 38(11):4440-4453. GAO Z X, WANG X L,XAING H, et al. Variation characteristics and potential sources of air pollutants during 2014-2017 in Wuhan[J]. Acta Scientiae Circumstantiae, 2018, 38(11):4440-4453(in Chinese).
[24] JUSTIN P, MILLER S, MARTIN S, JAMES J,et al. Seasonal contribution of mineral dust and other major components to particulate matter at two remote sites in Central Asia[J]. AtmospHeric Environment,2015,119:11-20. [25] BOGUSH A, STEGEMANN J A, WOOD I,et al. Element composition and mineralogical characterisation of air pollution control residue from UK energy-from-waste facilities[J]. Waste Management,2015,36:119-129. [26] 李帅. 矿物PM2.5对活体菌近膜物质作用及活性研究[D]. 绵阳:西南科技大学, 2016. LI S. Study on the effect and activity of mineral PM2.5 on living bacteria near membrane[D]. Mianyang:Southwest University of Science and Technology, 2016 (in Chinese).
[27] YANG X X, LI Y, LU A H,et al. Effect of Bacillus mucilaginosus D4B1 on the structure and soil-conservation-related properties of montmorillonite[J]. Applied Clay Science, 2016, 119:141-145. [28] MUELLER B. Experimental interactions between clay minerals and bacteria:A Review[J]. PedospHere, 2015, 25(6):799-810. -
点击查看大图
计量
- 文章访问数: 1265
- HTML全文浏览数: 1265
- PDF下载数: 24
- 施引文献: 0
下载:
