氧化石墨烯的制备、表征及其对发酵液中鼠李糖脂吸附分离特性
Preparation and characterization of graphene oxide and its adsorption and separation properties of rhamnolipid from fermentation broth
-
摘要: 以改良的Hummers氧化法制备了氧化石墨烯(GO),并研究其对铜绿假单胞菌发酵液中鼠李糖脂(Rha)的吸附分离特性.结果表明,通过Hummers氧化法,可使石墨粉氧化为GO,并形成层数很少甚至单层的GO片,使所得GO的比表面积较石墨粉显著增大.以所得GO对铜绿假单胞菌NY3发酵液中的Rha进行吸附,发现当发酵液的pH值为4.0、吸附温度为25℃时,Rha在GO表面的吸附可迅速平衡,其最大吸附量约为1.7 g·g-1.pH 13的氢氧化钠溶液可使Rha一次洗脱回收率为86.1%,是较佳的洗脱剂.重复利用实验表明,且所得的GO吸附剂可重复利用.Abstract: Graphene oxide (GO) was prepared using the method of improved Hummers, and its adsorption and separation properties of rhamnolipid from the fermentation broth of P. aeruginosa were studied. The results showed that, graphene has been oxidize and peeled to fewer layers with obviously lager specific surface area after oxidized by Hummer method. The adsorption of rhamnolipid on the surface of GO reached equilibrium quickly and the maximum adsorption amounts got to be 1.7 g·g-1 when pH and temperature of the fermentation broth was 4.0 and 25℃ respectively. The once desorption rate of rhamnolipid reached 86.1% using sodium hydroxide solution with pH of 13 as optimum desorption reagent. Meanwhile, the adsorbents of GO could be used recycling.
-
Key words:
- rhamnolipid /
- graphene oxide /
- adsorption /
- desorption
-
-
[1] ZULFIQAR ALI RAZA, MUHAMMAD SALEEM KHAN, et al. Evaluation of distant carbon sources in biosuactant production by a gamma ray-jnduced Pseudo-monas putida mutant[J].Pmcess Biochemjstry,2006,42(4):686-692. [2] 张天胜. 生物表面活性剂及其应用[M]. 北京:化学工业出版社, 2005. ZHANG T S. Biosurfactant and its application[M]. Beijing:Chemical Industry Press, 2005(in Chinese). [3] NEU T R, PORALLA K. Emulsifying agents from bacteria isolated during screening for cells with hydrophobic surfaces[J]. Applied Microbiology & Biotechnology, 1990, 32(5):521-525. [4] BODOUR A A, MILLERMAIER R M. Application of a modified drop-collapse technique for surfactant quantitation and screening of biosurfactant-producing microorganisms[J]. Journal of Microbiological Methods, 1998, 32(3):273-280. [5] DESAI J D, BANAT I M. Microbial production of surfactants and their commercial potential.[J]. Microbiology & Molecular Biology Reviews Mmbr, 1997, 61(1):47-64. [6] 徐仁扣, 赵安珍, 肖双成, 等. 农作物残体制备的生物质炭对水中亚甲基蓝的吸附作用[J]. 环境科学, 2012, 33(1):142-146. XU R K, ZHAO A Z, XIAO S C, et al. Adsorption of methylene blue in water by biomass charcoal prepared from crop residues[J]. Environmental Science, 2012, 33(1):142-146(in Chinese).
[7] 任方旭, 孙全莲, 董文博, 等. 发酵液中鼠李糖脂的活性炭吸附-酸沉淀分离[J]. 化学工程, 2016, 44(7):15-18. REN F X, SUN Q L, DONG W B, et al. Activated carbon adsorption-acid precipitation separation of rhamnolipid in fermentation broth[J]. Chemical Engineering, 2016, 44(7):15-18(in Chinese).
[8] XU D P, YOON S H, MOCHIDA I, et al. Synthesis of mesoporous carbon and its adsorption property to biomolecules[J]. Microporous & Mesoporous Materials, 2008, 115(3):461-468. [9] MARCANO D C, KOSYNKIN D V, BERLIN J M, et al. Improved Synthesis of Graphene Oxide[J]. ACS NANO, 2010, 4(8):4806-4814. [10] GEIM A K. Graphene:status and prospects.[J]. Science, 2009, 324(5934):1530-1534. [11] 常虹, 聂麦茜, 葛碧洲, 等. 铜绿假单胞菌NY3所产表面活性剂对原油降解的影响[J]. 环境工程学报, 2013, 7(2):771-776. CHANG H, NIE M Q, GE B Z, et al. Effect of surfactants produced by pseudomonas aeruginosa NY3 on crude oil degradation[J]. Chinese Journal of Environmental Engineering, 2013, 7(2):771-776(in Chinese).
[12] [13] CARMEN DOS SANTOS MENDES DE OLIVEIRA, BEZERRA M D S, MELCHUNA A M, et al. Recovery of rhamnolipids produced by pseudomonas aeruginosa using acidic precipitation, extraction, and adsorption on activated carbon[J]. Separation Science & Technology, 2013, 48(18):2852-2859. [14] NAGASHIMA A, NUKA K, ITOH H, et al. Electronic states of monolayer graphite formed on TiC(111) surface[J]. Surface Science Letters, 1993, 291(93):93-98. [15] CHEN W, YAN< L. Chemical reduction of graphene oxide to graphene by sulfur-containing compounds[J]. Journal of Physical Chemistry C, 2010, 114(47):19885-19890. [16] 朱洪胜. 碳源对NY3菌产Rha的影响及其在石油烃污染物降解中的作用研究[D]. 西安:西安建筑科技大学, 2017. ZHU H S. Effect of carbon source on Rha produced by NY3 strain and its role in the degradation of petroleum hydrocarbon pollutants[D]. Xi'an:Xi'an University of Architecture and Technology, 2017(in Chinese). -
![WeChat](http://eekw.rcees.ac.cn//eekw-data/hjhx/2019/10/PIC/wechat_cn0e7d167d-8a8a-41af-9718-3e951e9c300e.jpg)
计量
- 文章访问数: 1024
- HTML全文浏览数: 1024
- PDF下载数: 28
- 施引文献: 0