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丙烯酰胺对水环境中典型微藻的毒性效应

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徐文静, 张凯奇, 付强, 谭丽菊. 丙烯酰胺对水环境中典型微藻的毒性效应[J]. 环境化学, 2020, (1): 71-79. doi: 10.7524/j.issn.0254-6108.2019013101
引用本文: 徐文静, 张凯奇, 付强, 谭丽菊. 丙烯酰胺对水环境中典型微藻的毒性效应[J]. 环境化学, 2020, (1): 71-79. doi: 10.7524/j.issn.0254-6108.2019013101
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XU Wenjing, ZHANG Kaiqi, FU Qiang, TAN Liju. Toxic effects of acrylamide on phytoplankton in natural waters[J]. Environmental Chemistry, 2020, (1): 71-79. doi: 10.7524/j.issn.0254-6108.2019013101
Citation: XU Wenjing, ZHANG Kaiqi, FU Qiang, TAN Liju. Toxic effects of acrylamide on phytoplankton in natural waters[J]. Environmental Chemistry, 2020, (1): 71-79. doi: 10.7524/j.issn.0254-6108.2019013101
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丙烯酰胺对水环境中典型微藻的毒性效应

  • 1. 中国海洋大学化学化工学院, 青岛, 266100;

  • 2. 江苏省水文水资源勘测局盐城分局, 盐城, 224002;

  • 3. 盐城工学院环境科学与工程学院, 江苏省环境保护海涂生态与污染控制重点实验室, 盐城, 224051

    • 通讯作者: 谭丽菊, E-mail: lijutan@ouc.edu.cn
  • 基金项目:

    国家重点研发计划（2016YFC1402101）和国家科技支撑计划（2012BAF14B04）资助.

Toxic effects of acrylamide on phytoplankton in natural waters

  • 1. College of Chemistry and Chemical Engineering of Ocean University of China, Qingdao, 266100, China;

  • 2. Jiangsu Province Hydrology and Water Resources Investigation Bureau Yancheng Branch Municipal, Yancheng, 224002, China;

  • 3. Key Laboratory for Ecology and Pollution Control of Coastal Wetlands(Environmental Protection Department of Jiangsu Province), School of Environmental Science and Engineering, Yancheng Institute of Technology, Yancheng, 224051, China

    • Corresponding author: TAN Liju, lijutan@ouc.edu.cn
  • Fund Project: Supported by the National Key Research and Development Program (2016YFC1402101) and the National Science and Technology Support Program (2012BAF14B04).

  • 摘要: 作为可疑致癌物，丙烯酰胺（acrylamide，AM）是目前各国政府和广大民众普遍关注的重要污染物.为探究AM对水体生物的毒性效应及可能存在的生态风险，本文以海洋微藻东海原甲藻（Prorocentrum donghaiense）和淡水微藻莱茵衣藻（Chlamydomonas reinhardtii）为研究对象，采用室内培养法，测定了不同暴露浓度的AM对2种微藻生长、形态和生理状态的影响.结果显示，AM对2种藻类生长均有显著的抑制作用（P<0.05），96 h半抑制质量浓度（EC50）分别为22.79 mg·L-1和161.8 mg·L-1；最高无抑制浓度（NOEC）分别为1.04 mg·L-1和9.84 mg·L-1.不同微藻对AM胁迫的响应存在较大的差异性，与莱茵衣藻相比，东海原甲藻对AM更敏感.扫描电镜（SEM）和透射电镜（TEM）结果显示，当受到AM影响时，2种藻细胞严重变形，表现出塌陷、质壁分离、空泡数量增多和叶绿体片层结构少量断裂等现象；叶绿素含量和Fv/Fm（PSⅡ最大光化学量子产量）测试表明AM可以通过破坏微藻的光合系统而抑制光合作用.AM对东海原甲藻属于中毒性物质，对莱茵衣藻属于低毒性物质.
    Abstract: Acrylamide (AM), as a suspected carcinogen, is an important pollutant concerned by governments and the general public. To explore the toxic effects and associated mechanisms of AM on algae growth, marine algae (Prorocentrum donghaiense) and freshwater algae (Chlamydomonas reinhardtii) were investigated by determining various physiological and biochemical parameters, such as cell abundance, cell morphology, chlorophyll content and maximum photosystem Ⅱ (PSⅡ) quantum yield (Fv/Fm) under controlled laboratory conditions. The results demonstrated that the growth of the two algae were inhibited significantly by AM at 96 h (P < 0.05). Half maximal effect concentrations (EC50) for P. donghaiense and C. reinhardtii were 22.79 mg·L-1 and 161.8 mg·L-1, and no observed effect concentration (NOEC) for P. donghaiense and C. reinhardtii were 1.04 and 9.84 mg·L-1, respectively. P. donghaiense was more sensitive to the toxicity of AM, while C. reinhardtii was more tolerant. Scanning electron microscopy (SEM) and transmission electron microscopy (TEM) showed that 50 mg·L-1 AM exposure caused visible cell deformation, plasmolysis, and disintegration of chloroplasts. Chlorophyll content and Fv/Fm showed that AM could inhibit photosynthesis by destroying the photosynthetic system of microalgae. AM had medium toxicity to P. donghaiense, and low toxicity to C. reinhardtii.
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  • [1] WANG S Y, YU C P, PAN Y L, et al. Metabolomics analysis of serum from subjects after occupational exposure to acrylamide using UPLC-MS[J]. Molecular & Cellular Endocrinology, 2017, 444:67-75.
    [2] WEIDEBORG M, KALLQVIST T, ODEGARD K E, et al. Environmental risk assessment of acrylamide and methylolacrylamide from a grouting agent used in the tunnel construction of Romeriksporten, Norway[J]. Water Research, 2001, 35(11):2645-2652.
    [3] 张志荣, 李斌, 肖经纬, 等.丙烯酰胺的生物标志物研究概况[J]. 毒理学杂志, 2011,25(2):149-152.

    ZHANG Z R, LI B, XIAO J W, et al. Study on biomarkers of acrylamide[J]. Journal of Toxicology, 2011, 25(2):149-152(in Chinese).

    [4] 简龙海, 郑荣, 陈丹丹, 等.液相色谱-串联质谱法快速测定化妆品中的丙烯酰胺[J]. 分析试验室, 2015, 34(11):1317-1321.

    JIAN L H, ZHENG R, CHEN D D, et al. Rapid determination of acrylamide in cosmetics by liquid chromatography-tandem mass spectrometry[J]. Chinese Journal of Analysis Laboratory, 2015, 34(11):1317-1321(in Chinese).

    [5] 任祥祥, 李支薇, 王华, 等.GC-MS测定塑料中丙烯酰胺残留量[J]. 广东化工, 2016, 43(8):188-189.

    REN X X, LI Z W, WANG H, et al. Determination of acrylamide in plastics by gas chromatography mass-spectrometry[J]. Guangdong Chemical, 2016, 43(8):188-189(in Chinese).

    [6] JE Y. Dietary acrylamide intake and risk of endometrial cancer in prospective cohort studies[J]. Archives of Gynecology & Obstetrics, 2015, 291(6):1395-1401.
    [7] 陈芳, 袁媛, 刘野, 等.食品中丙烯酰胺研究进展[J]. 中国粮油学报, 2006, 21(2):124-128.

    CHEN F, YUAN Y, LIU Y, et al. Recent research on acrylamide in food[J]. Journal of the Chinese Cereals and Oils Association, 2006, 21(2):124-128(in Chinese).

    [8] HOGERVORST J G, PA V D B, GODSCHALK R W, et al. The influence of single nucleotide polymorphisms on the association between dietary acrylamide intake and endometrial cancer risk[J]. Scientific Reports, 2016, 6:34902
    [9] 樊振江, 孟楠.油炸食品中丙烯酰胺的形成及减少措施[J]. 现代食品, 2017, 3(6):27-29.

    FAN Z J, MENG N. Formation and reduction of acrylamide in fried food[J]. Modern Food, 2017, 3(6):27-29(in Chinese).

    [10] 阴永泼.姜粉对热加工食品中丙烯酰胺生成影响的研究[D]. 广州:华南理工大学, 2017, 1-3. YIN Y P. Effect of ginger powder on the formation of acrylamide in heat processed food[D]. Guangzhou:South China University of Technology, 2017, 1

    -3(in Chinese).

    [11] 丁茂柏.科学评估丙烯酰胺危害[J]. 中国职业医学, 2007, 34(1):61-64.

    DING M B. Scientifice valuation on the hazards of acrylamide[J]. China Occupational Medicine, 2007, 34(1):61-64(in Chinese).

    [12] HU Q, XU X, FU Y, et al. Rapid methods for detecting acrylamide in thermally processed foods:A review[J]. Food Control, 2015, 56:135-146.
    [13] HUANG M M, JIAO J J, WANG J, et al. Exposure to acrylamide induces cardiac developmental toxicity in zebrafish during cardiogenesis[J]. Environmental Pollution, 2017, 234:656-666.
    [14] AYUOB N, ALKARIM S, ELASSOULI S, et al. Effects of low dose acrylamide on the rat reproductive organs structure, fertility and gene integrity[J]. Asian Pacific Journal of Reproduction, 2015, 4(3):179-187.
    [15] 赵红, 江城梅, 赵文红, 等.丙烯酰胺致大鼠氧化损伤作用的实验研究[J]. 环境与健康杂志, 2008, 25(11):1013-1014.

    ZHAO H, JIANG C M, ZHAO W H, et al. Experimental study of oxidative damage induced by acrylamide in rats[J]. Journal of Envoirnment and Health, 2008, 25(11):1013-1014(in Chinese).

    [16] LEE K Y, SHIBUTANI M, KUROIWA K, et al. Chemoprevention of acrylamide toxity by antioxidative agents in rats-effective suppression of testicular toxicity by phenylethyl isothiocyanate[J]. Archices of Toxicology, 2005, 79(9):531-541.
    [17] CHEN J H, YANG C H, WANG Y S, et al. Acrylamide-induced mitochondria collapse and apoptosis in human astrocytoma cells[J]. Food & Chemical Toxicology, 2013, 51(1):446-452.
    [18] 曹秀明, 罗飞, 樊宇, 等.丙烯酰胺对斑马鱼各器官的毒性作用及生殖细胞的DNA损伤[J]. 生态毒理学报, 2016, 11(1):382-388.

    CAO X M, LUO F, FAN Y, et al. Toxic effects of acrylamide on zebrafish organs and DNA damage of germ cell[J]. Asian Journal of Ecotoxicology, 2016, 11(1):382-388(in Chinese).

    [19] 国家质检总局检验监管司.欧盟物质和混合物分类、标签和包装法规(CLP)指南[M]. 北京:中国标准出版社, 2010. General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China. EU Regulation on Classification, Labeling and Packaging of Substances and Mixtures (CLP)[M]. Beijing:Standards Press of China, 2010(in Chinese).
    [20] IARC. Monographs on the evaluation of carcinogenic risks to humans:some industrial chemicals[J]. Lyon:International Agency for Research on Cancer, 1994, 60:389-433.
    [21] 吴明珠, 何梅琳, 邹山梅, 等.纳米MgO对斜生栅藻的毒性效应及致毒机理[J]. 环境化学, 2015, 34(7):1259-1267.

    WU M Z, HE M L, ZOU S M, et al. Toxicities and mechanisms of MgO nanoparticles to Scenedesmus obliquus [J]. Environmental Chemistry, 2015, 34(7):1259-1267(in Chinese).

    [22] 徐田枚, 张洪涛, 吾甫尔·米吉提, 等.莱茵衣藻Nfr-4突变株中类胡萝卜素含量的变化及其对藻生长的影响[J]. 植物生理学报, 2007, 43(3):417-420.

    XU T M, ZHANG H T, GHOPUR MIJIT, et al. Change in carotenoid contents of Chlamydomonas reinhardtii mutant strain Nfr-4 and its effect on alge growth[J]. Plant Physiology Communications, 2007, 43(3):417-420(in Chinese).

    [23] 张鑫鑫, 唐学玺, 姜爽, 等.2, 2', 4, 4'-四溴联苯醚(BDE-47)对米氏凯伦藻的毒性效应[J]. 海洋环境科学, 2013, 32(4):491-496.

    ZHANG X X, TANG X X, JIANG S, et al. Toxic effect of 2, 2', 4, 4'-tetrabromodiphenyl ether (BDE-47) on Karenia mikimotoi in the different levels of biological organizations[J]. Marine Environmental Science, 2013, 32(4):491-496(in Chinese).

    [24] 解瑞丽, 周启星. 丙烯酰胺的环境暴露、生态行为与毒理效应研究进展[J]. 生态学杂志, 2013, 32(5):1347-1354.

    XIE R L, ZHOU Q X. Environmental exposure, ecological behavior, and toxicological effect of acrylamide:A review[J]. Chinese Journal of Ecology, 32(5):1347-1354(in Chinese).

    [25] 王自齐.有毒化学品卫生与安全实用手册[M]. 北京:化学工业出版社, 1993:88-89. WANG Z Q. Practical manual on hygiene and safety of toxic chemicals[M]. Beijing:Chemical Industry Press, 1993:88

    -89(in Chinese).

    [26] 徐英江, 黄会, 邹荣婕, 等.乙酰甲喹对小新月菱形藻、等鞭金藻3011的毒性效应[J]. 南方水产科学, 2017, 13(3):97-103.

    XU Y J, HUANG H, ZOU R J, et al. Toxic effect of mequindox on Nitzschia closterium f. minutissima and Isochrysis galbana parke 3011[J]. South China Fisheries Science, 2017, 13(3):97-103(in Chinese).

    [27] XUE Q N, WANG R, XU W J, et al. The stresses of allelochemicals isolated from culture solution of diatom Phaeodactylum tricornutum Bohlin on growth and physiology of two marine algae[J]. Aquatic Toxicology, 2018, 205:51-57.
    [28] SVERDRUP L E, KALLQVIST T, KELLEY A E, et al. Comparative toxicity of acrylic acid to marine and freshwater microalgae and the significance for environmental effects assessments[J]. Chemosphere, 2001, 45(4):653-658.
    [29] 姜思, 刘莹莹, 佟少明.4种常用抗生素对莱茵衣藻生长及光化学活性的影响[J]. 生物加工过程, 2017, 15(2):13-20.

    JIANG S, LIU Y Y, TONG S M. Effects of four antibiotics on growth and photochemical activities of Chlamydomonas reinhardtii[J]. Chinese Journal of Bioprocess Engineering, 2017, 15(2):13-20(in Chinese).

    [30] 刘莉莉, 孙凯峰, 李玉成, 等.壬基酚对四尾棚藻生长及群体形成的影响[J]. 安徽农业大学学报, 2016, 43(2):266-269.

    LIU L L, SUN K C, LI Y C, et al. Effect of nonylphenol on Scenedesmus quadricanda growth and colony formation[J]. Journal of Anhui Agricultural University, 2016, 43(2):266-269(in Chinese).

    [31] 王昭玉.叶绿素荧光参数对氮、磷限制的响应及其在赤潮生消过程中的变化特征研究[D]. 青岛:中国海洋大学, 2013. WANG Z Y. Responses of Chlorophyll a fluorescence parameter to nitrogen or phosphorus limitation and the variation of Chlorophyll a fluorescence parameter during the process of algal blooms[D]. Qingdao:Ocean University of China, 2013(in Chinese).
    [32] WANG R, HUA M, YU Y, et al. Evaluating the effects of allelochemical ferulic acid on Microcystis aeruginosa by pulse-amplitude-modulated (PAM) fluorometry and flow cytometry[J]. Chemosphere, 2016, 147:264-271.
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  • 收稿日期:  2019-01-31
  • 刊出日期:  2020-01-01

丙烯酰胺对水环境中典型微藻的毒性效应

    通讯作者: 谭丽菊, E-mail: lijutan@ouc.edu.cn
  • 1. 中国海洋大学化学化工学院, 青岛, 266100;
  • 2. 江苏省水文水资源勘测局盐城分局, 盐城, 224002;
  • 3. 盐城工学院环境科学与工程学院, 江苏省环境保护海涂生态与污染控制重点实验室, 盐城, 224051
  • 收稿日期:  2019-01-31
  • 网络出版日期:  2020-01-01
基金项目:

国家重点研发计划（2016YFC1402101）和国家科技支撑计划（2012BAF14B04）资助.

摘要: 作为可疑致癌物，丙烯酰胺（acrylamide，AM）是目前各国政府和广大民众普遍关注的重要污染物.为探究AM对水体生物的毒性效应及可能存在的生态风险，本文以海洋微藻东海原甲藻（Prorocentrum donghaiense）和淡水微藻莱茵衣藻（Chlamydomonas reinhardtii）为研究对象，采用室内培养法，测定了不同暴露浓度的AM对2种微藻生长、形态和生理状态的影响.结果显示，AM对2种藻类生长均有显著的抑制作用（P<0.05），96 h半抑制质量浓度（EC50）分别为22.79 mg·L-1和161.8 mg·L-1；最高无抑制浓度（NOEC）分别为1.04 mg·L-1和9.84 mg·L-1.不同微藻对AM胁迫的响应存在较大的差异性，与莱茵衣藻相比，东海原甲藻对AM更敏感.扫描电镜（SEM）和透射电镜（TEM）结果显示，当受到AM影响时，2种藻细胞严重变形，表现出塌陷、质壁分离、空泡数量增多和叶绿体片层结构少量断裂等现象；叶绿素含量和Fv/Fm（PSⅡ最大光化学量子产量）测试表明AM可以通过破坏微藻的光合系统而抑制光合作用.AM对东海原甲藻属于中毒性物质，对莱茵衣藻属于低毒性物质.

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