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王政, 张兴华, 张逦嘉, 霍鹏, 张元勋, 张阳. 大气颗粒物中环境持久性自由基的电子顺磁共振检测方法[J]. 环境化学, 2020, (2): 317-325. doi: 10.7524/j.issn.0254-6108.2019022703
引用本文: 王政, 张兴华, 张逦嘉, 霍鹏, 张元勋, 张阳. 大气颗粒物中环境持久性自由基的电子顺磁共振检测方法[J]. 环境化学, 2020, (2): 317-325. doi: 10.7524/j.issn.0254-6108.2019022703
shu
WANG Zheng, ZHANG Xinghua, ZHANG Lijia, HUO Peng, ZHANG Yuanxun, ZHANG Yang. Detection of environmentally persistent free radicals in atmospheric particulate matter by electron paramagnetic resonance[J]. Environmental Chemistry, 2020, (2): 317-325. doi: 10.7524/j.issn.0254-6108.2019022703
Citation: WANG Zheng, ZHANG Xinghua, ZHANG Lijia, HUO Peng, ZHANG Yuanxun, ZHANG Yang. Detection of environmentally persistent free radicals in atmospheric particulate matter by electron paramagnetic resonance[J]. Environmental Chemistry, 2020, (2): 317-325. doi: 10.7524/j.issn.0254-6108.2019022703
shu

大气颗粒物中环境持久性自由基的电子顺磁共振检测方法

  • 1. 中国科学院大学, 北京, 100049;

  • 2. 中国科学院怀柔生态环境综合观测研究站, 北京, 101400;

  • 3. 中国标准化研究院, 北京, 100191

    • 通讯作者: 张阳, E-mail: zhangyang@ucas.ac.cn
  • 基金项目:

    国家自然科学基金（21677145，21307129，21577150）和中国科学院大学校部教师与研究所合作专项基金（Y65201MY00）资助.

Detection of environmentally persistent free radicals in atmospheric particulate matter by electron paramagnetic resonance

  • 1. University of the Chinese Academy of Sciences, Beijing, 100049, China;

  • 2. Huairou Eco-Environmental Observatory, Chinese Academy of Sciences, Beijing, 101400, China;

  • 3. China National Institute of Standardization, Beijing, 100191, China

    • Corresponding author: ZHANG Yang, zhangyang@ucas.ac.cn
  • Fund Project: Supported by National Science Foundation of China (21677145,21307129,21577150) and University of the Chinese Academy of Sciences Special Fund for Cooperation with the Institute of Chinese Academy of Sciences (Y65201MY00).

  • 摘要: 大气颗粒物中的环境持久性自由基具有强氧化性，参与大气中诸多化学反应，影响空气质量，进而影响人体健康，因此对其定性、定量检测具有重要意义，然而现阶段并没有成熟有效的分析检测方法.本文利用电子顺磁共振波谱仪，对比大气颗粒物的3种进样方式，建立了便捷、有效的大气颗粒物中环境持久性自由基检测方法.首先，配制不同标准物质（或溶液），确定仪器的最佳检测参数和大气颗粒物的最低响应浓度（或质量）.然后，分别使用水洗脱法和有机溶剂提取法对大气颗粒物进行前处理，优化大气颗粒物的水洗脱和有机提取条件，对比大气颗粒物直接进样、水洗脱样和有机提取样的测定结果.最后，确定了上述3种进样方法的适用条件，即直接检测法适用于石英膜和Teflon膜样品，且操作简便，易于定量测定大气颗粒物中自由基的浓度；水洗脱法仅适用于Teflon膜样品，但需要已知颗粒物洗脱效率才能定量计算；有机溶剂提取法不能有效的提取的颗粒物上的自由基.
    Abstract: Due to their high oxidation potentials, environmentally persistent free radicals (EPFRs) in particulate matter (PM) can widely participate in atmospheric chemical reactions, leading to adverse effects on air quality and human health. While it is critical to develop a comprehensive understanding of EPFRs' role on these effects significant research gaps still exists due to limitations of analytical methods on EPFRs. To this end, a method for improved detection EPFRs in PM was developed by utilizing electron paramagnetic resonance (EPR) analysis of the extracted solutions using either deionized water or organic solvents. Multipoint calibration using standard solution was conducted to the instrument. Then, a series of experiments were carried out to derive optimal conditions of the solution extractions of the filter samples, which included sonication time, solvent type and concentration etc. The optimal instrument operation conditions for EPR analysis of the different types of samples were determined. The results indicated that using PM filter (quartz or TeflonTM) samples for EPFRs analysis through EPR was the best for both qualification and quantification purposes. On the other hand, it was challenging to quantify EPFRs through EPR analysis using solvent extraction from PM filter samples without knowing the extraction efficiency. The experiment results also showed that water elution method is only applicable to TeflonTM filter samples, while organic solvents didn't seem to be efficient in extracting the EPFRs from filter samples at all.
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  • 收稿日期:  2019-02-27
王政, 张兴华, 张逦嘉, 霍鹏, 张元勋, 张阳. 大气颗粒物中环境持久性自由基的电子顺磁共振检测方法[J]. 环境化学, 2020, (2): 317-325. doi: 10.7524/j.issn.0254-6108.2019022703
引用本文: 王政, 张兴华, 张逦嘉, 霍鹏, 张元勋, 张阳. 大气颗粒物中环境持久性自由基的电子顺磁共振检测方法[J]. 环境化学, 2020, (2): 317-325. doi: 10.7524/j.issn.0254-6108.2019022703
shu
WANG Zheng, ZHANG Xinghua, ZHANG Lijia, HUO Peng, ZHANG Yuanxun, ZHANG Yang. Detection of environmentally persistent free radicals in atmospheric particulate matter by electron paramagnetic resonance[J]. Environmental Chemistry, 2020, (2): 317-325. doi: 10.7524/j.issn.0254-6108.2019022703
Citation: WANG Zheng, ZHANG Xinghua, ZHANG Lijia, HUO Peng, ZHANG Yuanxun, ZHANG Yang. Detection of environmentally persistent free radicals in atmospheric particulate matter by electron paramagnetic resonance[J]. Environmental Chemistry, 2020, (2): 317-325. doi: 10.7524/j.issn.0254-6108.2019022703
shu

大气颗粒物中环境持久性自由基的电子顺磁共振检测方法

    通讯作者: 张阳, E-mail: zhangyang@ucas.ac.cn
  • 1. 中国科学院大学, 北京, 100049;
  • 2. 中国科学院怀柔生态环境综合观测研究站, 北京, 101400;
  • 3. 中国标准化研究院, 北京, 100191
  • 收稿日期:  2019-02-27
  • 网络出版日期:  2020-02-26
基金项目:

国家自然科学基金（21677145，21307129，21577150）和中国科学院大学校部教师与研究所合作专项基金（Y65201MY00）资助.

摘要: 大气颗粒物中的环境持久性自由基具有强氧化性，参与大气中诸多化学反应，影响空气质量，进而影响人体健康，因此对其定性、定量检测具有重要意义，然而现阶段并没有成熟有效的分析检测方法.本文利用电子顺磁共振波谱仪，对比大气颗粒物的3种进样方式，建立了便捷、有效的大气颗粒物中环境持久性自由基检测方法.首先，配制不同标准物质（或溶液），确定仪器的最佳检测参数和大气颗粒物的最低响应浓度（或质量）.然后，分别使用水洗脱法和有机溶剂提取法对大气颗粒物进行前处理，优化大气颗粒物的水洗脱和有机提取条件，对比大气颗粒物直接进样、水洗脱样和有机提取样的测定结果.最后，确定了上述3种进样方法的适用条件，即直接检测法适用于石英膜和Teflon膜样品，且操作简便，易于定量测定大气颗粒物中自由基的浓度；水洗脱法仅适用于Teflon膜样品，但需要已知颗粒物洗脱效率才能定量计算；有机溶剂提取法不能有效的提取的颗粒物上的自由基.

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