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典型羟基化多溴联苯醚(OH-PBDEs)小鼠肝微粒体的体外代谢及关键代谢CYP450酶系的研究

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张易曦, 杨霞, 王博, 张圣虎. 典型羟基化多溴联苯醚(OH-PBDEs)小鼠肝微粒体的体外代谢及关键代谢CYP450酶系的研究[J]. 环境化学, 2020, (1): 19-27. doi: 10.7524/j.issn.0254-6108.2019082103
引用本文: 张易曦, 杨霞, 王博, 张圣虎. 典型羟基化多溴联苯醚(OH-PBDEs)小鼠肝微粒体的体外代谢及关键代谢CYP450酶系的研究[J]. 环境化学, 2020, (1): 19-27. doi: 10.7524/j.issn.0254-6108.2019082103
shu
ZHANG Yixi, YANG Xia, WANG Bo, ZHANG Shenghu. Research on in vitro metabolism of typical hydroxylated polybrominated diphenyl ethers (OH-PBDEs) in mouse liver microsomes and the key CYP450 enzyme system[J]. Environmental Chemistry, 2020, (1): 19-27. doi: 10.7524/j.issn.0254-6108.2019082103
Citation: ZHANG Yixi, YANG Xia, WANG Bo, ZHANG Shenghu. Research on in vitro metabolism of typical hydroxylated polybrominated diphenyl ethers (OH-PBDEs) in mouse liver microsomes and the key CYP450 enzyme system[J]. Environmental Chemistry, 2020, (1): 19-27. doi: 10.7524/j.issn.0254-6108.2019082103
shu

典型羟基化多溴联苯醚(OH-PBDEs)小鼠肝微粒体的体外代谢及关键代谢CYP450酶系的研究

  • 1. 安顺学院, 安顺, 561000;

  • 2. 湖北省环境科学研究院, 武汉, 430072;

  • 3. 生态环境部南京环境科学研究所, 南京, 210042

    • 通讯作者: 张圣虎, E-mail: zsh@nies.org
  • 基金项目:

    国家自然科学基金（21407055）和江苏省自然科学基金（BK20140115）资助.

Research on in vitro metabolism of typical hydroxylated polybrominated diphenyl ethers (OH-PBDEs) in mouse liver microsomes and the key CYP450 enzyme system

  • 1. Anshun University, Anshun, 561000, China;

  • 2. Hubei Academy of Environmental Sciences, Wuhan, 430072, China;

  • 3. Nanjing Institute of Environmental Sciences, Ministry of Ecology and Environment, Nanjing, 210042, China

    • Corresponding author: ZHANG Shenghu, zsh@nies.org
  • Fund Project: Supported by the National Natural Science Foundation of China (21407055) and the Natural Science Foundation of Jiangsu Province (BK20140115).

  • 摘要: 羟基化多溴联苯醚（OH-PBDEs）是一类具有内分泌干扰效应的酚类化合物，本研究以小鼠肝脏微粒体作为研究对象，考察了4种典型OH-PBDEs （3-OH-BDE-47、5-OH-BDE-47、6-OH-BDE-47和2'-OH-BDE-68）的体外代谢.研究结果表明，4种OH-PBDEs在小鼠肝脏微粒体中均能被代谢；OH-PBDE结构上的醚键（O）、羟基（OH）和溴原子（Br）的相对位置会影响其代谢效率，OH位于苯环上醚键邻位更有利于其代谢，即6-OH-BDE-47表现出相对较高的代谢率；抑制剂实验发现CYP1A2和CYP3A4是4种典型OH-PBDEs主要的CYP450代谢亚型.
    Abstract: Hydroxylation polybrominateddiphenyl ethers (OH-PBDEs) are a class of phenolic compounds that have been found to be endocrine disruption, the in vitro metabolism of four typical kinds of OH-PBDEs (3-OH-BDE-47、5-OH-BDE-47、6-OH-BDE-47 and 2'-OH-BDE-68) were investigated using mouse liver microsome in this study. The results show that the four kinds of OH-PBDEs could be metabolized in the mouse liver microsome; the relative positions of ether bond (O), hydroxyl (OH) and bromine atom (Br) in OH-PBDE structure would affect its metabolic efficiency, and OH-PBDEs with hydroxyl group adjacent to the ortho position of the ether bond on the benzene ring showed faster metabolic rates, i.e. 6-OH-BDE-47 had a relatively high metabolic rate; Inhibitor experiments found that CYP1A2 and CYP3A4 were the major CYP450 metabolic subtypes of four typical OH-PBDEs.
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  • 收稿日期:  2019-08-21
  • 刊出日期:  2020-01-01
张易曦, 杨霞, 王博, 张圣虎. 典型羟基化多溴联苯醚(OH-PBDEs)小鼠肝微粒体的体外代谢及关键代谢CYP450酶系的研究[J]. 环境化学, 2020, (1): 19-27. doi: 10.7524/j.issn.0254-6108.2019082103
引用本文: 张易曦, 杨霞, 王博, 张圣虎. 典型羟基化多溴联苯醚(OH-PBDEs)小鼠肝微粒体的体外代谢及关键代谢CYP450酶系的研究[J]. 环境化学, 2020, (1): 19-27. doi: 10.7524/j.issn.0254-6108.2019082103
shu
ZHANG Yixi, YANG Xia, WANG Bo, ZHANG Shenghu. Research on in vitro metabolism of typical hydroxylated polybrominated diphenyl ethers (OH-PBDEs) in mouse liver microsomes and the key CYP450 enzyme system[J]. Environmental Chemistry, 2020, (1): 19-27. doi: 10.7524/j.issn.0254-6108.2019082103
Citation: ZHANG Yixi, YANG Xia, WANG Bo, ZHANG Shenghu. Research on in vitro metabolism of typical hydroxylated polybrominated diphenyl ethers (OH-PBDEs) in mouse liver microsomes and the key CYP450 enzyme system[J]. Environmental Chemistry, 2020, (1): 19-27. doi: 10.7524/j.issn.0254-6108.2019082103
shu

典型羟基化多溴联苯醚(OH-PBDEs)小鼠肝微粒体的体外代谢及关键代谢CYP450酶系的研究

    通讯作者: 张圣虎, E-mail: zsh@nies.org
  • 1. 安顺学院, 安顺, 561000;
  • 2. 湖北省环境科学研究院, 武汉, 430072;
  • 3. 生态环境部南京环境科学研究所, 南京, 210042
  • 收稿日期:  2019-08-21
  • 网络出版日期:  2020-01-01
基金项目:

国家自然科学基金（21407055）和江苏省自然科学基金（BK20140115）资助.

摘要: 羟基化多溴联苯醚（OH-PBDEs）是一类具有内分泌干扰效应的酚类化合物，本研究以小鼠肝脏微粒体作为研究对象，考察了4种典型OH-PBDEs （3-OH-BDE-47、5-OH-BDE-47、6-OH-BDE-47和2'-OH-BDE-68）的体外代谢.研究结果表明，4种OH-PBDEs在小鼠肝脏微粒体中均能被代谢；OH-PBDE结构上的醚键（O）、羟基（OH）和溴原子（Br）的相对位置会影响其代谢效率，OH位于苯环上醚键邻位更有利于其代谢，即6-OH-BDE-47表现出相对较高的代谢率；抑制剂实验发现CYP1A2和CYP3A4是4种典型OH-PBDEs主要的CYP450代谢亚型.

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