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利用毒代动力学模型研究氟虫腈在夹杂带丝蚓体内的手性选择性积累过程

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王顺慧, 李慧珍, 游静. 利用毒代动力学模型研究氟虫腈在夹杂带丝蚓体内的手性选择性积累过程[J]. 环境化学, 2020, (1): 12-18. doi: 10.7524/j.issn.0254-6108.2019012601
引用本文: 王顺慧, 李慧珍, 游静. 利用毒代动力学模型研究氟虫腈在夹杂带丝蚓体内的手性选择性积累过程[J]. 环境化学, 2020, (1): 12-18. doi: 10.7524/j.issn.0254-6108.2019012601
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WANG Shunhui, LI Huizhen, YOU Jing. Enantioselective bioaccumulation of waterborne fipronil in Lumbriculus variegatus with toxicokinetic modeling[J]. Environmental Chemistry, 2020, (1): 12-18. doi: 10.7524/j.issn.0254-6108.2019012601
Citation: WANG Shunhui, LI Huizhen, YOU Jing. Enantioselective bioaccumulation of waterborne fipronil in Lumbriculus variegatus with toxicokinetic modeling[J]. Environmental Chemistry, 2020, (1): 12-18. doi: 10.7524/j.issn.0254-6108.2019012601
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利用毒代动力学模型研究氟虫腈在夹杂带丝蚓体内的手性选择性积累过程

  • 1. 中国科学院广州地球化学研究所有机地球化学国家重点实验室, 广州, 510640;

  • 2. 暨南大学环境学院, 广东省环境污染与健康重点实验室, 广州, 510632;

  • 3. 西南石油大学化学化工学院, 四川省油气田应用化学重点实验室, 成都, 610500;

  • 4. 中国科学院大学, 北京, 100049

    • 通讯作者: 游静, E-mail: youjing@jnu.edu.cn
  • 基金项目:

    广东省自然科学基金（2017A030313065，2015A030310219，2016A030312009）资助.

Enantioselective bioaccumulation of waterborne fipronil in Lumbriculus variegatus with toxicokinetic modeling

  • 1. State Key Laboratory of Organic Geochemistry, Guangzhou Institute of Geochemistry, Chinese Academy of Sciences, Guangzhou, 510640, China;

  • 2. School of Environment and Guangdong Key Laboratory of Environmental Pollution and Health, Jinan University, Guangzhou, 510632, China;

  • 3. School of Chemistry and Chemical Engineering, Oil&Gas Field Applied Chemistry Key Laboratory of Sichuan Province, Southwest Petroleum University, Chengdu, 610500, China;

  • 4. University of Chinese Academy of Science, Beijing, 100049, China

    • Corresponding author: YOU Jing, youjing@jnu.edu.cn
  • Fund Project: Supported by the Natural Science Foundation of Guangdong Province (2017A030313065, 2015A030310219, 2016A030312009).

  • 摘要: 农药的生物积累直接影响其毒性，但是手性农药在生物体内的选择性积累过程仍不明确.本文在恒定环境水浓度的条件下，开展手性农药氟虫腈的外消旋体及其对映体在底栖无脊椎动物夹杂带丝蚓体内的生物积累实验.在96 h和72 h的吸收和消除实验过程中，测定生物体内的氟虫腈的外消旋体和对映体浓度随时间的变化情况，构建毒代动力学模型，获取吸收和消除速率常数.结果表明夹杂带丝蚓对R-氟虫腈的生物浓缩因子（1981 L·kg-1脂肪）比对S-氟虫腈（1748 L·kg-1脂肪）更大.R-氟虫腈和S-氟虫腈的吸收速率常数相当，分别为311±11 L·kg-1脂肪·h-1和313±13 L·kg-1脂肪·h-1，而R-氟虫腈的消除速率常数小于S-氟虫腈，分别为0.157±0.006 h-1和0.179±0.008 h-1，因此相对较慢的消除是导致R-氟虫腈在夹杂带丝蚓体内更高生物积累的主要原因.研究说明毒代动力学参数可有效阐释手性外源物质在生物体内选择性积累的差异.
    Abstract: Bioaccumulation of pesticides directly affects their toxicity, but the enantioselective bioaccumulation processes of chiral pesticides in organism remained unclear. In the present study, the bioaccumulation tests of fipronil, a chiral pesticide, in a benthic invertebrate Lumbriculus variegatus were conducted with constant water concentrations of fipronil throughout testing. During the courses of the 96 h absorption and 72 h elimination experiments, the concentrations of fipronil racemate and enantiomers were analyzed in organism over time. Then, toxicokinetic models were established and absorption and elimination rate constants of individual fipronil enantiomers were estimated from the toxicokinetic models. Results showed that L. variegatus had a higher bioconcentration factor to R-fipronil (1981 L·kg-1 lipid) than S-fipronil (1748 L·kg-1 lipid). The R- and S-fipronil had similar absorption rate constants, being 311±11 and 313±13 L·kg-1 lipid·h-1, respectively. On the other hand, the elimination rate constant of R-fipronil was smaller than that of S-fipronil, with the values being 0.157±0.006 h-1 and 0.179±0.008 h-1, respectively. As a consequence of slower elimination, the bioaccumulation potential of R-fipronil was greater than S-fipronil in the blackworms. The current study showed that toxicokinetic parameters could effectively elucidate enantioselective bioaccumulation of xenobiotics in the organisms.
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  • 收稿日期:  2019-01-26
  • 刊出日期:  2020-01-01
王顺慧, 李慧珍, 游静. 利用毒代动力学模型研究氟虫腈在夹杂带丝蚓体内的手性选择性积累过程[J]. 环境化学, 2020, (1): 12-18. doi: 10.7524/j.issn.0254-6108.2019012601
引用本文: 王顺慧, 李慧珍, 游静. 利用毒代动力学模型研究氟虫腈在夹杂带丝蚓体内的手性选择性积累过程[J]. 环境化学, 2020, (1): 12-18. doi: 10.7524/j.issn.0254-6108.2019012601
shu
WANG Shunhui, LI Huizhen, YOU Jing. Enantioselective bioaccumulation of waterborne fipronil in Lumbriculus variegatus with toxicokinetic modeling[J]. Environmental Chemistry, 2020, (1): 12-18. doi: 10.7524/j.issn.0254-6108.2019012601
Citation: WANG Shunhui, LI Huizhen, YOU Jing. Enantioselective bioaccumulation of waterborne fipronil in Lumbriculus variegatus with toxicokinetic modeling[J]. Environmental Chemistry, 2020, (1): 12-18. doi: 10.7524/j.issn.0254-6108.2019012601
shu

利用毒代动力学模型研究氟虫腈在夹杂带丝蚓体内的手性选择性积累过程

    通讯作者: 游静, E-mail: youjing@jnu.edu.cn
  • 1. 中国科学院广州地球化学研究所有机地球化学国家重点实验室, 广州, 510640;
  • 2. 暨南大学环境学院, 广东省环境污染与健康重点实验室, 广州, 510632;
  • 3. 西南石油大学化学化工学院, 四川省油气田应用化学重点实验室, 成都, 610500;
  • 4. 中国科学院大学, 北京, 100049
  • 收稿日期:  2019-01-26
  • 网络出版日期:  2020-01-01
基金项目:

广东省自然科学基金（2017A030313065，2015A030310219，2016A030312009）资助.

摘要: 农药的生物积累直接影响其毒性，但是手性农药在生物体内的选择性积累过程仍不明确.本文在恒定环境水浓度的条件下，开展手性农药氟虫腈的外消旋体及其对映体在底栖无脊椎动物夹杂带丝蚓体内的生物积累实验.在96 h和72 h的吸收和消除实验过程中，测定生物体内的氟虫腈的外消旋体和对映体浓度随时间的变化情况，构建毒代动力学模型，获取吸收和消除速率常数.结果表明夹杂带丝蚓对R-氟虫腈的生物浓缩因子（1981 L·kg-1脂肪）比对S-氟虫腈（1748 L·kg-1脂肪）更大.R-氟虫腈和S-氟虫腈的吸收速率常数相当，分别为311±11 L·kg-1脂肪·h-1和313±13 L·kg-1脂肪·h-1，而R-氟虫腈的消除速率常数小于S-氟虫腈，分别为0.157±0.006 h-1和0.179±0.008 h-1，因此相对较慢的消除是导致R-氟虫腈在夹杂带丝蚓体内更高生物积累的主要原因.研究说明毒代动力学参数可有效阐释手性外源物质在生物体内选择性积累的差异.

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