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太湖周边饮用水处理厂中抗生素抗性基因污染分布特征

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赵婉婷, 黄智峰, 郭雪萍, 徐挺, 尹大强. 太湖周边饮用水处理厂中抗生素抗性基因污染分布特征[J]. 环境化学, 2020, (12): 3271-3278. doi: 10.7524/j.issn.0254-6108.2019091502
引用本文: 赵婉婷, 黄智峰, 郭雪萍, 徐挺, 尹大强. 太湖周边饮用水处理厂中抗生素抗性基因污染分布特征[J]. 环境化学, 2020, (12): 3271-3278. doi: 10.7524/j.issn.0254-6108.2019091502
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ZHAO Wanting, HUANG Zhifeng, GUO Xueping, XU Ting, YIN Daqiang. Pollution and distribution characteristics of antibiotic resistance genes in drinking water treatment plants around Taihu Lake[J]. Environmental Chemistry, 2020, (12): 3271-3278. doi: 10.7524/j.issn.0254-6108.2019091502
Citation: ZHAO Wanting, HUANG Zhifeng, GUO Xueping, XU Ting, YIN Daqiang. Pollution and distribution characteristics of antibiotic resistance genes in drinking water treatment plants around Taihu Lake[J]. Environmental Chemistry, 2020, (12): 3271-3278. doi: 10.7524/j.issn.0254-6108.2019091502
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太湖周边饮用水处理厂中抗生素抗性基因污染分布特征

  • 同济大学环境科学与工程学院, 上海, 200092

    • 通讯作者: 徐挺, E-mail: xuting@tongji.edu.cn ;  尹大强, E-mail: yindq@tongji.edu.cn
  • 基金项目:

    水体污染控制与治理科技重大专项（2017ZX07201005）资助.

Pollution and distribution characteristics of antibiotic resistance genes in drinking water treatment plants around Taihu Lake

  • College of Environmental Science and Engineering, Tongji University, Shanghai, 200092, China

    • Corresponding authors: XU Ting, xuting@tongji.edu.cn ;  YIN Daqiang, yindq@tongji.edu.cn
  • Fund Project: Supported by the Major Science and Technology Program for Water Pollution Control and Treatment(2017ZX07201005).

  • 摘要: 为研究太湖周边不同水源的饮用水处理厂中抗生素抗性基因污染的分布特征，文章采用实时荧光定量PCR法对4个饮用水厂原水中12种ARGs（包括磺胺类sul1、sul2；四环素类tetC、tetW、β-内酰胺类blaTEM-1、blaOXA-1、blaampC；大环内酯类mphA、ermB；氨基糖苷类strA、aacC4；喹诺酮类qnrS）及Ⅰ类整合酶基因intⅠ1进行定性定量分析.结果表明，sul1、sul2、tetC、blaTEM-1、blaOXA-1、mphA、strA、qnrS和intⅠ1的检出率均为100%，其中磺胺类抗性基因sul1相对丰度最高（1.68×10-3—6.21×10-2），属于优势抗性基因.在4个水厂原水中，以太湖为水源的B水厂中检出ARGs的种类最多且相对丰度最高，而以长江为水源的D水厂中ARGs相对丰度水平最低.intⅠ1相对丰度和6种抗性基因相对丰度（sul2、tetC、blaTEM-1、blaOXA-1、mphA、qnrS）呈显著正相关（P<0.05），说明intⅠ1介导的ARGs水平转移是环境中微生物获得耐药性的重要途径.根据基因丰度检测结果结合水源分析，太湖区域水体ARGs污染水平高于长江下游水体.本研究充分阐明了太湖和长江下游原水中抗生素抗性基因的污染特征，为今后饮用水处理中对抗生素抗性基因的去除提供理论依据和数据支撑.
    Abstract: In order to investigate the distribution and pollution status of antibiotic resistance genes (ARGs) in drinking water treatment plants (DWTPs) fed by Taihu Lake and the lower reaches of the Yangtze River around Taihu Lake, quantitative real-time PCR were applied to analyze the 12 ARGs (including sulfonamide resistance genes sul1, sul2; tetracycline resistance genes tetC, tetW; β-lactams resistance genes blaTEM-1, blaOXA-1, blaampC;macrolide resistance genes mphA、ermB; aminoglycoside resistance genes strA、aacC4; quinolone resistance gene qnrS and class Ⅰ integron gene intⅠ1 of raw water in 4 drinking water treatment plants. In this study, sul1, sul2, tetC, blaTEM-1, blaOXA-1, mphA, strA, qnrS and intⅠ1 were investigated in all water samples. Sul1 had the highest relative abundance (1.68×10-3— 6.21×10-2), and was the predominant resistance gene in 12 ARGs. The results suggested that B had the most abundant ARGs and the highest total relative abundance genes, while D had the lowest among the 4 DWTPs. There was a significant positive correlation between intⅠ1 and several ARGs(sul2, tetC, blaTEM-1, blaOXA-1, mphA, qnrS)(P<0.05), indicating that mediated horizontal gene transfer by intⅠ1 was a crucial way to acquire resistance. According to the concentration and water source analysis, the pollution status of Taihu was found to be worse than lower reaches of Yangtze river. Overall, our results revealed the occurrence and abundance of antibiotic resistance genes in the Taihu Lake and the lower reaches of the Yangtze River, and provided important information in theory and data support to contribute to their elimination in drinking water treatment.
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  • 收稿日期:  2019-09-15

太湖周边饮用水处理厂中抗生素抗性基因污染分布特征

  • 收稿日期:  2019-09-15
基金项目:

水体污染控制与治理科技重大专项（2017ZX07201005）资助.

摘要: 为研究太湖周边不同水源的饮用水处理厂中抗生素抗性基因污染的分布特征，文章采用实时荧光定量PCR法对4个饮用水厂原水中12种ARGs（包括磺胺类sul1、sul2；四环素类tetC、tetW、β-内酰胺类blaTEM-1、blaOXA-1、blaampC；大环内酯类mphA、ermB；氨基糖苷类strA、aacC4；喹诺酮类qnrS）及Ⅰ类整合酶基因intⅠ1进行定性定量分析.结果表明，sul1、sul2、tetC、blaTEM-1、blaOXA-1、mphA、strA、qnrS和intⅠ1的检出率均为100%，其中磺胺类抗性基因sul1相对丰度最高（1.68×10-3—6.21×10-2），属于优势抗性基因.在4个水厂原水中，以太湖为水源的B水厂中检出ARGs的种类最多且相对丰度最高，而以长江为水源的D水厂中ARGs相对丰度水平最低.intⅠ1相对丰度和6种抗性基因相对丰度（sul2、tetC、blaTEM-1、blaOXA-1、mphA、qnrS）呈显著正相关（P<0.05），说明intⅠ1介导的ARGs水平转移是环境中微生物获得耐药性的重要途径.根据基因丰度检测结果结合水源分析，太湖区域水体ARGs污染水平高于长江下游水体.本研究充分阐明了太湖和长江下游原水中抗生素抗性基因的污染特征，为今后饮用水处理中对抗生素抗性基因的去除提供理论依据和数据支撑.

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