一株芴降解菌的分离鉴定及其对多环芳烃的降解广谱性研究
Research on isolation and identification of a fluorine-degrading strain and its broad-spectrum property for degradation of polycyclic aromatic hydrocarbons
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摘要: 从某焦化厂活性污泥中筛选出一株芴的优势降解菌株W-1，经形态学观察、生理生化实验和16S rDNA基因序列分析，确定W-1为粪产碱杆菌。在接种量10%（V/V），初始芴浓度40 mg/L，pH 7.0，温度30℃的条件下，接种培养11 d后，菌株W-1对芴的降解效率达到87.8%。菌株W-1对多环芳烃的降解具有广谱性，在分别含有20 mg/L的(艹屈)(chrysene)、芘、蒽和菲的培养体系中，该菌对(艹屈)、芘、蒽和菲的降解效率分别可达95.5%、71%、85.7% 和80%。研究结果表明，该菌可作为修复多环芳烃复合污染环境的优良微生物资源。Abstract: A predominant fluorine-degrading strain W-1 was isolated and screened from activated sludge of a coking plant. The strain was identified as Alcaligenes faecalis by the results of morphological observation, physio-biochemical test and 16S rDNA gene sequence analysis. Under the conditions of inoculation amount of 10% (V/V), initial concentration of fluorene of 40 mg/L, pH 7.0 and temperature of 30℃, the degradation efficiency of fluorene by the strain W-1 reached 87.8% within 11 days. The degradable PAHs as substrates for metabolism of the strain W-1 are diversiform. In the cultivation system solely containing chrysene, pyrene, anthracene or phenanthrene of 20 mg/L, the degradation efficiency of chrysene, pyrene, anthracene and phenanthrene by the strain W-1 reached 95.5%、71.0%、85.7% and 80.0%, respectively. The results showed that the strain W-1 could be used as superordinary microorganism resource to remedy the environment complexly polluted by polycyclic aromatic hydrocarbons.
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