密度梯度分离/免疫荧光技术检测再生水中隐孢子虫和贾第鞭毛虫

陈智敏; 张昱; 杨敏; 肖淑敏; 张冬青; 李红岩; 郭瑞光

环境工程学报

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陈智敏, 张昱, 杨敏, 肖淑敏, 张冬青, 李红岩, 郭瑞光. 密度梯度分离/免疫荧光技术检测再生水中隐孢子虫和贾第鞭毛虫[J]. 环境工程学报, 2011, 5(5): 982-986.

引用本文:

Chen Zhimin, Zhang Yu, Yang Min, Xiao Shumin, Zhang Dongqing, Li Hongyan, Guo Ruiguang. Detection of Cryptosporidium and Giardia in reclaimed wastewater systems using immunofluorescence assay and density gradient separation[J]. Chinese Journal of Environmental Engineering, 2011, 5(5): 982-986.

Citation:

Chen Zhimin, Zhang Yu, Yang Min, Xiao Shumin, Zhang Dongqing, Li Hongyan, Guo Ruiguang. Detection of Cryptosporidium and Giardia in reclaimed wastewater systems using immunofluorescence assay and density gradient separation[J]. Chinese Journal of Environmental Engineering, 2011, 5(5): 982-986.

密度梯度分离/免疫荧光技术检测再生水中隐孢子虫和贾第鞭毛虫

1.
1 .西安建筑科技大学环境与市政工程学院，西安 710000
2.
2 .中国科学院生态环境研究中心环境水质学国家重点实验室，北京 100085
基金项目:
国家“水体污染控制与治理”科技重大专项（2008ZX07314-003

008）

国家自然科学基金资助项目（50778171)
中图分类号: X832

Detection of Cryptosporidium and Giardia in reclaimed wastewater systems using immunofluorescence assay and density gradient separation

1.
1.College of Environmental and Municipal Engineering， Xi’an University of Architecture and Technology， Xi’an 710000，China
2.
2. State Key Laboratory of Environmental Aquatic Chemistry， Research Center for Eco-Environmental Sciences，Chinese Academy of Sciences，Beijing 100085， China
Fund Project:

摘要: 采用乙酸乙酯漂浮方法和酸溶解法分别解决了再生水进水中有机杂质以及再生水中剩余絮凝剂干扰隐孢子虫和贾第鞭毛虫检测的关键问题，在此基础上，建立了成本远低于EPA 1623法的隐孢子虫和贾第鞭毛虫检测方法的密度梯度分离/免疫荧光技术。利用该方法对我国北方2个城市的污水再生处理系统进行了检测，再生水进出水的回收率分别为18%~31%卵囊和24%~95%包囊，能够满足检测要求。结果表明，在5个再生水系统的9个进水样品中，隐孢子虫的阳性率为89%，平均浓度为26个/10 L；贾第鞭毛虫的阳性率为100%，平均浓度为138个/10 L。再生水出水中也有两虫检出。
- 再生水 /
- 隐孢子虫 /
- 贾第鞭毛虫 /
- 密度梯度分离
Abstract: For the reclaimed influents and the reclaimed effluents with excess coagulant， the ethyl acetate floatation and acid dissociation were suggested to be added to enhance the purification and increase the recovery， respectively. Then an immunofluorescence assay and density gradient separation method for detection of Cryptosporidium oocysts and Giardia cysts， was developed in reclaimed wastewater systems. The Cryptosporidium oocysts and Giardia cysts of reclaimed wastewaters were detected in T and B cities in north China using immunofluorescence assay and density gradient separation method. The acceptable recoveries obtained from reclaimed influents and effluents were in the range of 18%~31% oocysts and 24%~95% cysts. The results showed that Cryptosporidium were detected in 89% samples with mean concentration of 26 oocysts/10 L，while Giardia were detected in 100% samples with mean concentration of 138 cysts/10 L in the influents of 5 reclaimed wastewater systems. Besides，Cryptosporidium oocysts and Giardia cysts were also present in the effluents of some reclaimed wastewater systems.
- reclaimed water /
- Cryptosporidium /
- Giardia /
- density gradient separation

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Citation:

Chen Zhimin, Zhang Yu, Yang Min, Xiao Shumin, Zhang Dongqing, Li Hongyan, Guo Ruiguang. Detection of Cryptosporidium and Giardia in reclaimed wastewater systems using immunofluorescence assay and density gradient separation[J]. Chinese Journal of Environmental Engineering, 2011, 5(5): 982-986.

密度梯度分离/免疫荧光技术检测再生水中隐孢子虫和贾第鞭毛虫

1. 1 .西安建筑科技大学环境与市政工程学院，西安 710000
2. 2 .中国科学院生态环境研究中心环境水质学国家重点实验室，北京 100085

收稿日期: 2011-03-01

基金项目:

国家“水体污染控制与治理”科技重大专项（2008ZX07314-003

008）

国家自然科学基金资助项目（50778171)

关键词:

摘要: 采用乙酸乙酯漂浮方法和酸溶解法分别解决了再生水进水中有机杂质以及再生水中剩余絮凝剂干扰隐孢子虫和贾第鞭毛虫检测的关键问题，在此基础上，建立了成本远低于EPA 1623法的隐孢子虫和贾第鞭毛虫检测方法的密度梯度分离/免疫荧光技术。利用该方法对我国北方2个城市的污水再生处理系统进行了检测，再生水进出水的回收率分别为18%~31%卵囊和24%~95%包囊，能够满足检测要求。结果表明，在5个再生水系统的9个进水样品中，隐孢子虫的阳性率为89%，平均浓度为26个/10 L；贾第鞭毛虫的阳性率为100%，平均浓度为138个/10 L。再生水出水中也有两虫检出。

English Abstract

Detection of Cryptosporidium and Giardia in reclaimed wastewater systems using immunofluorescence assay and density gradient separation

1. 1.College of Environmental and Municipal Engineering， Xi’an University of Architecture and Technology， Xi’an 710000，China
2. 2. State Key Laboratory of Environmental Aquatic Chemistry， Research Center for Eco-Environmental Sciences，Chinese Academy of Sciences，Beijing 100085， China

Received Date: 2011-03-01

Fund Project:

Keywords:

Abstract: For the reclaimed influents and the reclaimed effluents with excess coagulant， the ethyl acetate floatation and acid dissociation were suggested to be added to enhance the purification and increase the recovery， respectively. Then an immunofluorescence assay and density gradient separation method for detection of Cryptosporidium oocysts and Giardia cysts， was developed in reclaimed wastewater systems. The Cryptosporidium oocysts and Giardia cysts of reclaimed wastewaters were detected in T and B cities in north China using immunofluorescence assay and density gradient separation method. The acceptable recoveries obtained from reclaimed influents and effluents were in the range of 18%~31% oocysts and 24%~95% cysts. The results showed that Cryptosporidium were detected in 89% samples with mean concentration of 26 oocysts/10 L，while Giardia were detected in 100% samples with mean concentration of 138 cysts/10 L in the influents of 5 reclaimed wastewater systems. Besides，Cryptosporidium oocysts and Giardia cysts were also present in the effluents of some reclaimed wastewater systems.

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密度梯度分离/免疫荧光技术检测再生水中隐孢子虫和贾第鞭毛虫

Detection of Cryptosporidium and Giardia in reclaimed wastewater systems using immunofluorescence assay and density gradient separation

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密度梯度分离/免疫荧光技术检测再生水中隐孢子虫和贾第鞭毛虫

English Abstract

Detection of Cryptosporidium and Giardia in reclaimed wastewater systems using immunofluorescence assay and density gradient separation

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