PCR-DGGE技术解析A2/O工艺好氧单元中微生物群落结构

吕晶华; 马挺; 郑先强; 段云霞; 李召雨

环境工程学报

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吕晶华, 马挺, 郑先强, 段云霞, 李召雨. PCR-DGGE技术解析A2/O工艺好氧单元中微生物群落结构[J]. 环境工程学报, 2012, 6(6): 1907-1914.

引用本文:

吕晶华, 马挺, 郑先强, 段云霞, 李召雨. PCR-DGGE技术解析A2/O工艺好氧单元中微生物群落结构[J]. 环境工程学报, 2012, 6(6): 1907-1914.

Lü Jinghua, Ma Ting, Zheng Xianqiang, Duan Yunxia, Li Zhaoyu. Application of PCR-DGGE to analysis of microbial community structure in aerobic unit of A2/O process[J]. Chinese Journal of Environmental Engineering, 2012, 6(6): 1907-1914.

Citation:

Lü Jinghua, Ma Ting, Zheng Xianqiang, Duan Yunxia, Li Zhaoyu. Application of PCR-DGGE to analysis of microbial community structure in aerobic unit of A2/O process[J]. Chinese Journal of Environmental Engineering, 2012, 6(6): 1907-1914.

PCR-DGGE技术解析A2/O工艺好氧单元中微生物群落结构

1.
1．天津市环境保护科学研究院研发部，天津 300191； 2．天津市联合环保工程设计有限公司，天津 300191
2.
3．南开大学生命科学学院石油微生物国家重点实验室，天津 300171
基金项目:
国家水体污染控制与治理科技重大专项（2008ZX07314-001）

国家自然科学基金资助项目（51178311）
中图分类号: X172

Application of PCR-DGGE to analysis of microbial community structure in aerobic unit of A2/O process

1.
1.Research and Development Department of Tianjin Academy of Environmental Sciences， Tianjin 300191， China； 2.Tianjin Federation Environment Engineering Design Co.， Ltd.， Tianjin 300191， China
2.
3. State Key Laboratory of Petroleum Microbiology， College of Life Sciences， Nankai University， Tianjin 300171， China
Fund Project:

摘要: GE方法，追踪了汉沽工业废水处理中好氧工艺的活性污泥系统中微生物群落结构动态变化过程及其微生物群落结构组成。研究结果表明：系统中的微生物群落结构随水质变化而变化，随着培养时间的延长，微生物群落结构趋于稳定，分别属于5大类群，与γ、δ、α、ε变形杆菌(Proteobacterias)、芽孢杆菌(Bacilli)的亲缘关系较近。其中γ变形杆菌是该废水处理过程中的主要菌群，包括Pseudomonas sp.、Rheinheimera sp.、Citrobacter sp.、Klebsiella sp.、Enterbacteriaceae、Stenotrophomonas maltophilia、Acinetobacter。在整个系统中uncultured Pseudomonas sp.、Halobacillus sp.、Pseudomonas sp.、Pseudomonas stutzeri、Acinetobacter sp.可稳定存在于系统中，为该污水处理系统中的优势微生物。因此，提高Halobacillus sp.、Pseudomonas sp.、Pseudomonas stutzeri、Acinetobacter sp.菌属在系统中的数量和质量，有利于提高废水生化处理的效果。
- PCR-DGGE /
- 活性污泥系统 /
- 微生物群落结构 /
- 优势菌
Abstract: The dynamic process and the microbial community structure in the aerobic activated sludge system of the industrial wastewater treatment were traced by the PCR-DGGE technology. The results show that the microbial community structure changes with the water quality in the system and becomes stable as the incubation time increasing. The microbial community structure is mainly composed of five categories which respectively have close relationships with the γ， δ， α， εProteobacterias， Bacilli. γProteobacterium is the main microbial community， which including Pseudomonas sp.， Rheinheimera sp.， Citrobacter sp.， Klebsiella sp.， Enterbacteriaceae， Stenotrophomonas maltophilia， Acinetobacter. Five bacteria including uncultured Pseudomonas sp.， Halobacillus sp.， Pseudomonas sp.， Pseudomonas stutzeri， Acinetobacter sp. can exist stably in the system and become dominant microorganisms. So the effect of wastewater treatment can be improved by improving quantity and quality of the dominant microorganisms in the system.
- PCR-DGGE /
- activated sludge system /
- microbial community structure /
- dominant microorganisms

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吕晶华, 马挺, 郑先强, 段云霞, 李召雨. PCR-DGGE技术解析A2/O工艺好氧单元中微生物群落结构[J]. 环境工程学报, 2012, 6(6): 1907-1914.

引用本文:

吕晶华, 马挺, 郑先强, 段云霞, 李召雨. PCR-DGGE技术解析A2/O工艺好氧单元中微生物群落结构[J]. 环境工程学报, 2012, 6(6): 1907-1914.

Citation:

PCR-DGGE技术解析A2/O工艺好氧单元中微生物群落结构

1. 1．天津市环境保护科学研究院研发部，天津 300191； 2．天津市联合环保工程设计有限公司，天津 300191
2. 3．南开大学生命科学学院石油微生物国家重点实验室，天津 300171

收稿日期: 2011-06-02

基金项目:

国家水体污染控制与治理科技重大专项（2008ZX07314-001）

国家自然科学基金资助项目（51178311）

关键词:

摘要: GE方法，追踪了汉沽工业废水处理中好氧工艺的活性污泥系统中微生物群落结构动态变化过程及其微生物群落结构组成。研究结果表明：系统中的微生物群落结构随水质变化而变化，随着培养时间的延长，微生物群落结构趋于稳定，分别属于5大类群，与γ、δ、α、ε变形杆菌(Proteobacterias)、芽孢杆菌(Bacilli)的亲缘关系较近。其中γ变形杆菌是该废水处理过程中的主要菌群，包括Pseudomonas sp.、Rheinheimera sp.、Citrobacter sp.、Klebsiella sp.、Enterbacteriaceae、Stenotrophomonas maltophilia、Acinetobacter。在整个系统中uncultured Pseudomonas sp.、Halobacillus sp.、Pseudomonas sp.、Pseudomonas stutzeri、Acinetobacter sp.可稳定存在于系统中，为该污水处理系统中的优势微生物。因此，提高Halobacillus sp.、Pseudomonas sp.、Pseudomonas stutzeri、Acinetobacter sp.菌属在系统中的数量和质量，有利于提高废水生化处理的效果。

English Abstract

Application of PCR-DGGE to analysis of microbial community structure in aerobic unit of A2/O process

1. 1.Research and Development Department of Tianjin Academy of Environmental Sciences， Tianjin 300191， China； 2.Tianjin Federation Environment Engineering Design Co.， Ltd.， Tianjin 300191， China
2. 3. State Key Laboratory of Petroleum Microbiology， College of Life Sciences， Nankai University， Tianjin 300171， China

Received Date: 2011-06-02

Fund Project:

Keywords:

Abstract: The dynamic process and the microbial community structure in the aerobic activated sludge system of the industrial wastewater treatment were traced by the PCR-DGGE technology. The results show that the microbial community structure changes with the water quality in the system and becomes stable as the incubation time increasing. The microbial community structure is mainly composed of five categories which respectively have close relationships with the γ， δ， α， εProteobacterias， Bacilli. γProteobacterium is the main microbial community， which including Pseudomonas sp.， Rheinheimera sp.， Citrobacter sp.， Klebsiella sp.， Enterbacteriaceae， Stenotrophomonas maltophilia， Acinetobacter. Five bacteria including uncultured Pseudomonas sp.， Halobacillus sp.， Pseudomonas sp.， Pseudomonas stutzeri， Acinetobacter sp. can exist stably in the system and become dominant microorganisms. So the effect of wastewater treatment can be improved by improving quantity and quality of the dominant microorganisms in the system.

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PCR-DGGE技术解析A2/O工艺好氧单元中微生物群落结构

Application of PCR-DGGE to analysis of microbial community structure in aerobic unit of A2/O process

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PCR-DGGE技术解析A2/O工艺好氧单元中微生物群落结构

English Abstract

Application of PCR-DGGE to analysis of microbial community structure in aerobic unit of A2/O process

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