摘要:
采用日本青鳉幼鱼及胚胎暴露评估染织排水的毒性效应。4个测试水样采自染织工厂排水口。96h急性毒性试验表明:1和2号水样对幼鱼急性毒性效应不明显,3和4号水样对幼鱼具有急性毒性效应,毒性单位分别为0.47、0.53、9.93和1.68TUa,1和2号水样为微毒,3号水样为中毒,4号水样为低毒。14d胚胎幼鱼慢性毒性试验表明:1、3和4号水样引起胚胎死亡,胚胎孵化的无观察效应浓度(NOEC)分别为90%、20%和33%。4号水样52%和64%试验组延长了胚胎孵化的时间并与对照组产生了显著性差异(p<0.05)。2号水样严重抑制胚胎孵化,胚胎孵化率的NOEC为2.5%,其EC25为39.2%。水样的慢性毒性单位分别为:1.11、40、5和3.03TUc。鱼苗经3d暴露后,仅1和3号水样中的试验组对幼鱼产生致死效应。存活率NOEC分别为90%、20%、10%和64%。
Abstract:
The effect of toxicity of dye effluent was evaluated by Japanese medaka(Oryzias latipes) from embryo to fry.4 samples were obtained from the discharge of dye factories.The results of 96 h-acute toxicity test showed that sample 3 and 4 had acute toxicity to juvenile fish whereas acute toxicity of sample 1 and 2 was not significant.The acute toxicity units of 4 samples were 0.47,0.53,9.93 and 1.68 TUa,respectively.Sample 1 and 2 are mildly toxic,sample 3 was medium toxic and sample 4 was low toxic.For 14 d chronic toxicity,the results showed that the lethal of embryos in sample 1,3 and 4 were observed,the no observed effect concentrations(NOEC) for hatching rate were 90%,20% and 33%,respectively.The hatching time are delayed in group of 52% and 64% of sample 4 and significantly different with the control(p>0.05).The inhibition of hatching of embryo were significant,NOEC for hatching rate was 2.5%,and EC25 was 39.2%.The chronic toxicity units of the four samples were 1.11,40,5 and 3.03 TUc,respectively.The fish fry were resulted in death by the treatment groups of sample 1 and 3 after 3 d exposure.NOEC for survival rate of fish fry are 90%,20%,10% and 64%,respectively.