纳米二氧化钛对浮萍生长和生理特征的影响
Effects of TiO2-NPs on Growth and Physiological Characteristics of Lemna minor
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摘要: 为了揭示纳米二氧化钛（TiO2-NPs）对水生植物生长和生理特征的影响，选取浮萍为受试物种，输入锐钛矿型、金红石型和P25混合型（m（锐钛矿）：m（金红石）=4：1）3种晶型的TiO2-NPs，测定不同浓度（CK：0 mg·L-1，T25：25 mg·L-1，T50：50 mg·L-1，T75：75 mg·L-1，T100：100 mg·L-1）对浮萍叶片数、叶面积、叶绿素含量、超氧化物歧化酶（SOD）活性、过氧化物酶（POD）活性和过氧化氢酶（CAT）活性的影响。结果表明：（1）锐钛矿处理中，不同浓度处理均抑制浮萍生长，T25和T50处理对叶绿素a含量产生抑制作用，T75和T100处理对叶绿素a含量产生促进作用；不同浓度处理对叶绿素b和总叶绿素含量产生抑制作用，对SOD和POD活性产生促进作用；T25和T75处理对CAT活性产生促进作用，T50和T100处理对CAT活性产生抑制作用。（2）金红石处理中，不同浓度处理促进浮萍生长；对叶绿素a含量产生促进作用；对叶绿素b和总叶绿素含量产生抑制作用，T25、T50和T100处理对SOD活性产生促进作用，T75处理对SOD活性产生抑制作用；不同浓度处理对POD活性产生促进作用；T25、T75和T100处理对CAT活性产生抑制作用，T50处理对CAT活性产生促进作用。（3）P25处理中，不同浓度处理促进浮萍生长，对叶绿素a含量产生促进作用，对叶绿素b含量产生抑制作用，随着浓度的增加，抑制作用增强；T25、T50和T100处理对总叶绿素含量产生促进作用，T75处理对总叶绿素含量产生抑制作用；对SOD和POD活性均产生促进作用，T25、T50和T100处理对CAT活性产生抑制作用，T75处理对CAT活性产生促进作用。锐钛矿型TiO2-NPs会抑制浮萍生长，金红石型和P25混合型TiO2-NPs会促进浮萍生长。Abstract: To reveal the influence of TiO2-NPs on the growth and physiological characteristics of aquatic plants, Lemna minor was selected as the subject species and the three crystal types of TiO2-NPs (CK:0 mg·L-1, T25:25 mg·L-1, T50:50 mg·L-1, T75:75 mg·L-1, T100:100 mg·L-1) were input, which are anatase type, rutile type and P25 mixture type (anatase:rutile=4:1), and the effect of TiO2-NPs on the indexes of leaf number, leaf area, chlorophyll content, the activities of superoxide dismutase (SOD), peroxidase (POD) and catalase (CAT) of Lemna minor were measured. The results indicate that:(1) In the anatase type treated group, the growth of Lemna minor was inhibited by different concentration treatment, T25 and T50 treatment inhibited chlorophyll a content, T75 and T100 treatment promoted chlorophyll a content; different concentration treatment inhibited chlorophyll b and total chlorophyll content, and promoted SOD and POD activity; T25 and T75 treatment promoted CAT activity, T50 and T100 treatment inhibited CAT activity. (2) In the rutile type, treatment with different concentrations promoted the growth of Lemna minor, promoted chlorophyll a content and inhibited chlorophyll b and total chlorophyll content; T25, T50 and T100 treatment promoted SOD activity, T75 treatment inhibited SOD activity; different concentration treatment promoted POD activity; T25, T75 and T100 treatment inhibited CAT activity, T50 treatment promoted CAT activity. (3) In the P25 mixture type, treatment with different concentrations promoted the growth of Lemna minor, promoted chlorophyll a content, inhibited chlorophyll b content and with the increase of concentration, the inhibitory effect increased; T25, T50 and T100 treatment promoted total chlorophyll content, T75 treatment inhibited total chlorophyll content; SOD and POD activity were promoted by different concentration treatment; T25, T50 and T100 treatment inhibited CAT activity, T75 treatment promoted CAT activity.
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Key words:
- TiO2-NPs /
- Lemna minor /
- growth inhibition /
- enzyme activity
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