草木樨在汞胁迫下的遗传分化研究

郭洪泽; 张秋华; 段毅豪; 李翠兰; 王钰婧

生态毒理学报

草木樨在汞胁迫下的遗传分化研究

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郭洪泽, 张秋华, 段毅豪, 李翠兰, 王钰婧. 草木樨在汞胁迫下的遗传分化研究[J]. 生态毒理学报, 2011, 6(3): 326-330.

引用本文:

郭洪泽, 张秋华, 段毅豪, 李翠兰, 王钰婧. 草木樨在汞胁迫下的遗传分化研究[J]. 生态毒理学报, 2011, 6(3): 326-330.

Guo Hongze, Zhang Qiuhua, Duan Yihao, Li Cuilan, Wang Yujing. Genetic Differentiation among Melilotus officinalis Seedlings Exposed to Mercury[J]. Asian journal of ecotoxicology, 2011, 6(3): 326-330.

Citation:

Guo Hongze, Zhang Qiuhua, Duan Yihao, Li Cuilan, Wang Yujing. Genetic Differentiation among Melilotus officinalis Seedlings Exposed to Mercury[J]. Asian journal of ecotoxicology, 2011, 6(3): 326-330.

草木樨在汞胁迫下的遗传分化研究

1. 山西大学环境与资源学院;
2. 山西大学生命科学学院

作者简介: Guo Hongze(1984-),male,research field:genetic ecotoxicology,E-mail:ott227@163.com

通讯作者: 段毅豪, E-mail: duany@sxu.edu.cn ;

基金项目:
Fund for Returned Scholars from Abroad of Shanxi(2010)

Shanxi Natural Science Fund(No.2006011015)
中图分类号: X171.5

Genetic Differentiation among Melilotus officinalis Seedlings Exposed to Mercury

1. School of Environmental Science and Resources of Shanxi University, Taiyuan, Shanxi 030006, China;
2. School of Life Science of Shanxi University, Taiyuan, Shanxi 030006, China

Corresponding author: Duan Yihao, duany@sxu.edu.cn ;

Fund Project: Fund for Returned Scholars from Abroad of Shanxi(2010) Shanxi Natural Science Fund(No.2006011015)

摘要: 以草木樨(Melilotus officinalis)种子为研究对象,将其暴露于0、20、40和60mg·L^-1浓度的氯化汞中进行萌发,并对存活的幼苗利用水平淀粉凝胶电泳进行等位酶分析。结果表明,草木樨在Me和Pgi基因座具有多态性,其平均的等位基因数为3.0基因座-1,杂合性为0.332-0.342,草木樨符合种群遗传研究的条件。Roger’s遗传距离聚类分析表明草木樨在对照组和汞暴露存活个体组间存在明显的遗传距离分化。草木樨的某些基因型极有可能对汞污染存抗性作用,汞暴露通过定向选择导致遗传分化。研究表明,野化的草木樨是在黄土高原地区进行矿区植物修复的理想潜在物种。
- 草木樨 /
- 氯化汞 /
- 水平淀粉凝胶电泳 /
- Roger's遗传距离聚类分析 /
- 遗传分化
Abstract: To examine the genotypic responses of forage grass species to acute exposure to mercury chloride(HgCl₂),Melilotus officinalis seed was selected for germinating in concentrations of 0,20,40 and 60 mg·L^-1 HgCl₂.Polymeric allozyme genotypes of seedlings were tested using horizontal starch gel electrophoresis.The allozyme analysis showed high polymorphism at PGI and ME with the mean allele frequency of 3.0 and the mean observed heterozygosity of 0.332-0.342 for M.officinalis,suggesting the suitability of the species for allozyme analysis on genetic adaptations.The cluster analysis using Roger’s genetic distance showed remarkable deviation between the surviving individuals and the control group.This indicates that certain genotypes of Melilotus officinalis may be more resistant to acute HgCl₂ under laboratory conditions.The genetic differentiations are observed via directional selection process.The richness in genetic variation in wild Melilotus officinalis makes it being a potentially promising species in soil remediation in mining areas in loess plateau.
- Melilotus officinalis /
- mercury chloride /
- horizontal starch gel electrophoresis /
- Roger's genetic distance cluster analysis /
- genetic differentiation

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郭洪泽, 张秋华, 段毅豪, 李翠兰, 王钰婧. 草木樨在汞胁迫下的遗传分化研究[J]. 生态毒理学报, 2011, 6(3): 326-330.

引用本文:

郭洪泽, 张秋华, 段毅豪, 李翠兰, 王钰婧. 草木樨在汞胁迫下的遗传分化研究[J]. 生态毒理学报, 2011, 6(3): 326-330.

Guo Hongze, Zhang Qiuhua, Duan Yihao, Li Cuilan, Wang Yujing. Genetic Differentiation among Melilotus officinalis Seedlings Exposed to Mercury[J]. Asian journal of ecotoxicology, 2011, 6(3): 326-330.

Citation:

草木樨在汞胁迫下的遗传分化研究

通讯作者: 段毅豪, E-mail: duany@sxu.edu.cn ;

作者简介: Guo Hongze(1984-),male,research field:genetic ecotoxicology,E-mail:ott227@163.com
1. 山西大学环境与资源学院;

2. 山西大学生命科学学院

收稿日期: 2010-10-31

基金项目:

Fund for Returned Scholars from Abroad of Shanxi(2010)

Shanxi Natural Science Fund(No.2006011015)

关键词:

摘要: 以草木樨(Melilotus officinalis)种子为研究对象,将其暴露于0、20、40和60mg·L^-1浓度的氯化汞中进行萌发,并对存活的幼苗利用水平淀粉凝胶电泳进行等位酶分析。结果表明,草木樨在Me和Pgi基因座具有多态性,其平均的等位基因数为3.0基因座-1,杂合性为0.332-0.342,草木樨符合种群遗传研究的条件。Roger’s遗传距离聚类分析表明草木樨在对照组和汞暴露存活个体组间存在明显的遗传距离分化。草木樨的某些基因型极有可能对汞污染存抗性作用,汞暴露通过定向选择导致遗传分化。研究表明,野化的草木樨是在黄土高原地区进行矿区植物修复的理想潜在物种。

English Abstract

Genetic Differentiation among Melilotus officinalis Seedlings Exposed to Mercury

Corresponding author: Duan Yihao, duany@sxu.edu.cn ;

1. School of Environmental Science and Resources of Shanxi University, Taiyuan, Shanxi 030006, China;
2. School of Life Science of Shanxi University, Taiyuan, Shanxi 030006, China

Received Date: 2010-10-31

Fund Project: Fund for Returned Scholars from Abroad of Shanxi(2010) Shanxi Natural Science Fund(No.2006011015)

Keywords:

Melilotus officinalis /
mercury chloride /
horizontal starch gel electrophoresis /
Roger's genetic distance cluster analysis /
genetic differentiation

Abstract: To examine the genotypic responses of forage grass species to acute exposure to mercury chloride(HgCl₂),Melilotus officinalis seed was selected for germinating in concentrations of 0,20,40 and 60 mg·L^-1 HgCl₂.Polymeric allozyme genotypes of seedlings were tested using horizontal starch gel electrophoresis.The allozyme analysis showed high polymorphism at PGI and ME with the mean allele frequency of 3.0 and the mean observed heterozygosity of 0.332-0.342 for M.officinalis,suggesting the suitability of the species for allozyme analysis on genetic adaptations.The cluster analysis using Roger’s genetic distance showed remarkable deviation between the surviving individuals and the control group.This indicates that certain genotypes of Melilotus officinalis may be more resistant to acute HgCl₂ under laboratory conditions.The genetic differentiations are observed via directional selection process.The richness in genetic variation in wild Melilotus officinalis makes it being a potentially promising species in soil remediation in mining areas in loess plateau.

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草木樨在汞胁迫下的遗传分化研究

作者简介: Guo Hongze(1984-),male,research field:genetic ecotoxicology,E-mail:ott227@163.com

通讯作者: 段毅豪, E-mail: duany@sxu.edu.cn ;

Genetic Differentiation among Melilotus officinalis Seedlings Exposed to Mercury

Corresponding author: Duan Yihao, duany@sxu.edu.cn ;

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草木樨在汞胁迫下的遗传分化研究

通讯作者: 段毅豪, E-mail: duany@sxu.edu.cn ;

作者简介: Guo Hongze(1984-),male,research field:genetic ecotoxicology,E-mail:ott227@163.com 1. 山西大学环境与资源学院; 2. 山西大学生命科学学院

English Abstract

Genetic Differentiation among Melilotus officinalis Seedlings Exposed to Mercury

Corresponding author: Duan Yihao, duany@sxu.edu.cn ;

全文HTML

目录

作者简介: Guo Hongze(1984-),male,research field:genetic ecotoxicology,E-mail:ott227@163.com
1. 山西大学环境与资源学院;

2. 山西大学生命科学学院