摘要:
为给中国花鲈(Lateolabrax maculatus)养殖提供污染生物学的理论数据,研究了中国花鲈幼鱼的铜离子中毒症状和半致死浓度(LC50);检测了幼鱼肝脏的谷胱甘肽(GSH)和丙二醛(MDA)含量及超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、谷丙转氨酶(GPT)和谷草转氨酶(GOT)活力;分析了铜离子胁迫对幼鱼的红细胞总核异率和微核率的影响。结果表明:铜离子对幼鱼24、48、72和96h的LC50分别为1.85、1.66、1.48和0.86mg·L-1。濒死的幼鱼肝脏组织溶解性坏死,鳃部组织出现大量的血窦,幼鱼肝脏的GSH和MDA含量及SOD、GPT和GOT活力对铜离子胁迫反应敏感,而CAT活力则反应不敏感。在铜离子浓度为0.42mg·L-1的浓度组中暴露96h,幼鱼的总核异率和微核率显著高于对照组(p<0.05)。以上结果说明铜离子胁迫损害了中国花鲈幼鱼肝脏的抗氧化系统、肝脏组织和鳃部组织,并具有一定的遗传毒性。
Abstract:
In order to provide data of pollution biology for the perch(Lateolabrax maculatus) culture,the toxic symptom and LC50 were studied in the juvenile perch exposed to Cu2+.The levels of GSH and MDA and activities of SOD,CAT,GOT and GPT were examined;The effects of Cu2+ stress on the total dyskaryosis and micronuclear rates of the juvenile erythrocytes were analyzed.The results showed that LC50 for juvenile exposed to Cu2+ for 24,48,72 and 96 h was 1.85,1.66,1.48 and 0.86 mg·L-1,respectively.The liver tissue of agonal individuals necrosed and a mass of blood sinuses occurred in the gill tissue.GSH,MDA,SOD,GPT and GOT in the liver were sensitive,while CAT was not sensitive to Cu2+ stress.Exposed to Cu2+ at concentration of 0.42 mg L-1 for 96 h,total dyskaryosis and micronuclear rates of the juvenile erythrocytes in the treated groups were significantly higher(p<0.05) than that in the control.Cu2+ stress not only damaged the liver antioxidant system,liver and gill tissues,but also had definite genetic toxity to the juvenile perch.
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