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丙烯酰胺对水环境中典型微藻的毒性效应

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徐文静, 张凯奇, 付强, 谭丽菊. 丙烯酰胺对水环境中典型微藻的毒性效应[J]. 环境化学, 2020, (1): 71-79. doi: 10.7524/j.issn.0254-6108.2019013101
引用本文: 徐文静, 张凯奇, 付强, 谭丽菊. 丙烯酰胺对水环境中典型微藻的毒性效应[J]. 环境化学, 2020, (1): 71-79. doi: 10.7524/j.issn.0254-6108.2019013101
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XU Wenjing, ZHANG Kaiqi, FU Qiang, TAN Liju. Toxic effects of acrylamide on phytoplankton in natural waters[J]. Environmental Chemistry, 2020, (1): 71-79. doi: 10.7524/j.issn.0254-6108.2019013101
Citation: XU Wenjing, ZHANG Kaiqi, FU Qiang, TAN Liju. Toxic effects of acrylamide on phytoplankton in natural waters[J]. Environmental Chemistry, 2020, (1): 71-79. doi: 10.7524/j.issn.0254-6108.2019013101
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丙烯酰胺对水环境中典型微藻的毒性效应

  • 1. 中国海洋大学化学化工学院, 青岛, 266100;

  • 2. 江苏省水文水资源勘测局盐城分局, 盐城, 224002;

  • 3. 盐城工学院环境科学与工程学院, 江苏省环境保护海涂生态与污染控制重点实验室, 盐城, 224051

    • 通讯作者: 谭丽菊, E-mail: lijutan@ouc.edu.cn
  • 基金项目:

    国家重点研发计划（2016YFC1402101）和国家科技支撑计划（2012BAF14B04）资助.

Toxic effects of acrylamide on phytoplankton in natural waters

  • 1. College of Chemistry and Chemical Engineering of Ocean University of China, Qingdao, 266100, China;

  • 2. Jiangsu Province Hydrology and Water Resources Investigation Bureau Yancheng Branch Municipal, Yancheng, 224002, China;

  • 3. Key Laboratory for Ecology and Pollution Control of Coastal Wetlands(Environmental Protection Department of Jiangsu Province), School of Environmental Science and Engineering, Yancheng Institute of Technology, Yancheng, 224051, China

    • Corresponding author: TAN Liju, lijutan@ouc.edu.cn
  • Fund Project: Supported by the National Key Research and Development Program (2016YFC1402101) and the National Science and Technology Support Program (2012BAF14B04).

  • 摘要: 作为可疑致癌物，丙烯酰胺（acrylamide，AM）是目前各国政府和广大民众普遍关注的重要污染物.为探究AM对水体生物的毒性效应及可能存在的生态风险，本文以海洋微藻东海原甲藻（Prorocentrum donghaiense）和淡水微藻莱茵衣藻（Chlamydomonas reinhardtii）为研究对象，采用室内培养法，测定了不同暴露浓度的AM对2种微藻生长、形态和生理状态的影响.结果显示，AM对2种藻类生长均有显著的抑制作用（P<0.05），96 h半抑制质量浓度（EC50）分别为22.79 mg·L-1和161.8 mg·L-1；最高无抑制浓度（NOEC）分别为1.04 mg·L-1和9.84 mg·L-1.不同微藻对AM胁迫的响应存在较大的差异性，与莱茵衣藻相比，东海原甲藻对AM更敏感.扫描电镜（SEM）和透射电镜（TEM）结果显示，当受到AM影响时，2种藻细胞严重变形，表现出塌陷、质壁分离、空泡数量增多和叶绿体片层结构少量断裂等现象；叶绿素含量和Fv/Fm（PSⅡ最大光化学量子产量）测试表明AM可以通过破坏微藻的光合系统而抑制光合作用.AM对东海原甲藻属于中毒性物质，对莱茵衣藻属于低毒性物质.
    Abstract: Acrylamide (AM), as a suspected carcinogen, is an important pollutant concerned by governments and the general public. To explore the toxic effects and associated mechanisms of AM on algae growth, marine algae (Prorocentrum donghaiense) and freshwater algae (Chlamydomonas reinhardtii) were investigated by determining various physiological and biochemical parameters, such as cell abundance, cell morphology, chlorophyll content and maximum photosystem Ⅱ (PSⅡ) quantum yield (Fv/Fm) under controlled laboratory conditions. The results demonstrated that the growth of the two algae were inhibited significantly by AM at 96 h (P < 0.05). Half maximal effect concentrations (EC50) for P. donghaiense and C. reinhardtii were 22.79 mg·L-1 and 161.8 mg·L-1, and no observed effect concentration (NOEC) for P. donghaiense and C. reinhardtii were 1.04 and 9.84 mg·L-1, respectively. P. donghaiense was more sensitive to the toxicity of AM, while C. reinhardtii was more tolerant. Scanning electron microscopy (SEM) and transmission electron microscopy (TEM) showed that 50 mg·L-1 AM exposure caused visible cell deformation, plasmolysis, and disintegration of chloroplasts. Chlorophyll content and Fv/Fm showed that AM could inhibit photosynthesis by destroying the photosynthetic system of microalgae. AM had medium toxicity to P. donghaiense, and low toxicity to C. reinhardtii.
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  • 收稿日期:  2019-01-31
  • 刊出日期:  2020-01-01

丙烯酰胺对水环境中典型微藻的毒性效应

    通讯作者: 谭丽菊, E-mail: lijutan@ouc.edu.cn
  • 1. 中国海洋大学化学化工学院, 青岛, 266100;
  • 2. 江苏省水文水资源勘测局盐城分局, 盐城, 224002;
  • 3. 盐城工学院环境科学与工程学院, 江苏省环境保护海涂生态与污染控制重点实验室, 盐城, 224051
  • 收稿日期:  2019-01-31
  • 网络出版日期:  2020-01-01
基金项目:

国家重点研发计划（2016YFC1402101）和国家科技支撑计划（2012BAF14B04）资助.

摘要: 作为可疑致癌物，丙烯酰胺（acrylamide，AM）是目前各国政府和广大民众普遍关注的重要污染物.为探究AM对水体生物的毒性效应及可能存在的生态风险，本文以海洋微藻东海原甲藻（Prorocentrum donghaiense）和淡水微藻莱茵衣藻（Chlamydomonas reinhardtii）为研究对象，采用室内培养法，测定了不同暴露浓度的AM对2种微藻生长、形态和生理状态的影响.结果显示，AM对2种藻类生长均有显著的抑制作用（P<0.05），96 h半抑制质量浓度（EC50）分别为22.79 mg·L-1和161.8 mg·L-1；最高无抑制浓度（NOEC）分别为1.04 mg·L-1和9.84 mg·L-1.不同微藻对AM胁迫的响应存在较大的差异性，与莱茵衣藻相比，东海原甲藻对AM更敏感.扫描电镜（SEM）和透射电镜（TEM）结果显示，当受到AM影响时，2种藻细胞严重变形，表现出塌陷、质壁分离、空泡数量增多和叶绿体片层结构少量断裂等现象；叶绿素含量和Fv/Fm（PSⅡ最大光化学量子产量）测试表明AM可以通过破坏微藻的光合系统而抑制光合作用.AM对东海原甲藻属于中毒性物质，对莱茵衣藻属于低毒性物质.

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