PM2.5急性暴露致运动大鼠BALF和心脏氧化损伤的相关性研究
Study on Relationship between BALF and Cardiac Oxidative Damage Induced by Acute PM2.5 Exposure in Exercise Rats
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摘要: 收集体育场馆内细颗粒物，对大鼠经气管滴注细颗粒物悬液并进行递增负荷运动，以探究体育场馆内细颗粒物对运动大鼠肺和心脏组织的氧化损伤作用。实验选取32只雄性SPF级Wistar大鼠，随机分为对照组、高剂量染毒组（30 mg·kg-1）、中剂量染毒组（15 mg·kg-1）、低剂量染毒组（7.5 mg·kg-1），采用一次性气管滴注染毒后，进行递增负荷跑台训练，运动结束后测定大鼠肺泡灌洗液、心脏组织中的谷胱甘肽过氧化物酶（GSH-Px）、总超氧化物歧化酶（T-SOD）、过氧化氢酶（CAT）、丙二醛（MDA）和活性氧（ROS）指标。与对照组相比，随着细颗粒物染毒剂量的增加，肺泡灌洗液和心肌组织中的GSH-Px、T-SOD表现出剂量相关性降低，MDA和ROS则呈剂量相关性升高，均有显著差异。这表明，细颗粒物可以对急性运动大鼠的肺和心脏的抗氧化系统造成氧化损伤。Abstract: To study the oxidative damage of PM2.5 in sports venues on lung and heart tissues of rats by collecting fine particulate matter in sports venues and establishing a rat model of acute exposed sports injury by intratracheal drip. Thirty-two male Wistar SPF rats were randomly divided into control group (CG), high-dose group (30 mg·kg-1) (HG), middle-dose group (15 mg·kg-1) (MG), low-dose group (7.5 mg·kg-1) (LG). After one-time tracheal infusion, the rats were trained on treadmill with incremental load. The alveolar lavage fluid (BALF) and the valley of heart tissue were collected. The indicators of glutathione peroxidase (GSH-Px), total superoxide dismutase (T-SOD), catalase (CAT), malondialdehyde (MDA) and reactive oxygen species (ROS) were determined. Compared with EC group, GSH-Px, T-SOD in BALF and myocardial tissue showed a dose-dependent decrease, while MDA and ROS showed a dose-dependent increase with the increase of PM2.5 dose. PM2.5 can damage the function of antioxidant system of lung and heart in acute exercise rats.
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Key words:
- fine particles /
- exercise rats /
- antioxidant enzymes /
- stadiums
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