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功能宏基因组学在新型抗生素耐药基因研究中的应用进展

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何荣, 原珂, 林里, 杨颖, 邹世春, 栾天罡, 陈保卫. 功能宏基因组学在新型抗生素耐药基因研究中的应用进展[J]. 环境化学, 2019, (7): 1548-1556. doi: 10.7524/j.issn.0254-6108.2018090702
引用本文: 何荣, 原珂, 林里, 杨颖, 邹世春, 栾天罡, 陈保卫. 功能宏基因组学在新型抗生素耐药基因研究中的应用进展[J]. 环境化学, 2019, (7): 1548-1556. doi: 10.7524/j.issn.0254-6108.2018090702
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HE Rong, YUAN Ke, LIN Li, YANG Ying, ZOU Shichun, LUAN Tiangang, CHEN Baowei. Functional metagenomics: One of the most robust tools for discovering new antibiotics resistance genes[J]. Environmental Chemistry, 2019, (7): 1548-1556. doi: 10.7524/j.issn.0254-6108.2018090702
Citation: HE Rong, YUAN Ke, LIN Li, YANG Ying, ZOU Shichun, LUAN Tiangang, CHEN Baowei. Functional metagenomics: One of the most robust tools for discovering new antibiotics resistance genes[J]. Environmental Chemistry, 2019, (7): 1548-1556. doi: 10.7524/j.issn.0254-6108.2018090702
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功能宏基因组学在新型抗生素耐药基因研究中的应用进展

  • 1. 中山大学海洋科学学院, 广州, 510275;

  • 2. 中山大学生命科学院, 广州, 510275;

  • 3. 中山大学环境科学与工程学院, 广州, 510275

    • 通讯作者: 陈保卫, E-mail: chenbw5@mail.sysu.edu.cn
  • 基金项目:

    国家自然科学基金（21777198）资助.

Functional metagenomics: One of the most robust tools for discovering new antibiotics resistance genes

  • 1. School of Marine Sciences, Sun Yat-Sen University, Guangzhou, 510275, China;

  • 2. School of Life Sciences, Sun Yat-Sen University, Guangzhou, 510275, China;

  • 3. School of Environmental Science and Engineering, Sun Yat-sen University, Guangzhou, 510275, China

    • Corresponding author: CHEN Baowei, chenbw5@mail.sysu.edu.cn
  • Fund Project: Supported by National Natural Science Foundation of China (21777198).

  • 摘要: 抗生素耐药性是二十一世纪人类面对的最严峻的环境健康问题之一.抗生素耐药基因（Antibiotics resistance genes，ARGs）被认为是一类新型环境污染物.当前针对ARGs的主要研究方法有细菌分离和培养法、聚合酶链式反应（Polymerase Chain Reaction，PCR）法、和宏基因组法.然而，仅有功能宏基因组方法能发现新型的ARGs.功能宏基因组方法利用新一代测序技术的高通量的特性，结合分子生物学技术和功能筛选构建有关抗生素耐药性的基因库，通过生物信息学分析高效地发现新型ARGs.本文综述了近来利用功能宏基因组技术筛选新型ARGs的相关研究进展，总结了功能宏基因学相关的技术和方法的优势和限制，并展望了功能宏基因学方法进一步发展的方向.
    Abstract: Antibiotic resistance is one of the greatest challenges on the public health in the world. Currently, antibiotic resistance genes (ARGs) have been regarded as a group of emerging environmental contaminants. The main analytical approaches of ARGs include isolation and incubation of bacterial strains, PCR, and new generation high-throughput sequencing-based metagenomic methods. However, functional metagenomic approach is a robust tool to find the new ARGs that have never been identified. This approach combines the high throughput of new generation sequencing platforms with function identification of constructing gene libraries, which is able to efficiently seek the novel ARGs without any of prior knowledge about the function of them. In this paper, we reviewed research progress on the application of functional metagenomics in the discovery of novel ARGs and summarized the advantages and flaws of current technologies and methods related to functional metagenomics. In final, we provided the perspectives of the future improvement on functional metagenomics.
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  • 收稿日期:  2018-09-07

功能宏基因组学在新型抗生素耐药基因研究中的应用进展

    通讯作者: 陈保卫, E-mail: chenbw5@mail.sysu.edu.cn
  • 1. 中山大学海洋科学学院, 广州, 510275;
  • 2. 中山大学生命科学院, 广州, 510275;
  • 3. 中山大学环境科学与工程学院, 广州, 510275
  • 收稿日期:  2018-09-07
基金项目:

国家自然科学基金（21777198）资助.

摘要: 抗生素耐药性是二十一世纪人类面对的最严峻的环境健康问题之一.抗生素耐药基因（Antibiotics resistance genes，ARGs）被认为是一类新型环境污染物.当前针对ARGs的主要研究方法有细菌分离和培养法、聚合酶链式反应（Polymerase Chain Reaction，PCR）法、和宏基因组法.然而，仅有功能宏基因组方法能发现新型的ARGs.功能宏基因组方法利用新一代测序技术的高通量的特性，结合分子生物学技术和功能筛选构建有关抗生素耐药性的基因库，通过生物信息学分析高效地发现新型ARGs.本文综述了近来利用功能宏基因组技术筛选新型ARGs的相关研究进展，总结了功能宏基因学相关的技术和方法的优势和限制，并展望了功能宏基因学方法进一步发展的方向.

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