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外加碳源对抗生素诱导大肠杆菌Hormesis效应的调控作用

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沈洪艳, 韩冬旭, 杨雷, 李双江, 孙昊宇, 唐量. 外加碳源对抗生素诱导大肠杆菌Hormesis效应的调控作用[J]. 生态毒理学报, 2023, 18(5): 156-164. doi: 10.7524/AJE.1673-5897.20230106002
引用本文: 沈洪艳, 韩冬旭, 杨雷, 李双江, 孙昊宇, 唐量. 外加碳源对抗生素诱导大肠杆菌Hormesis效应的调控作用[J]. 生态毒理学报, 2023, 18(5): 156-164. doi: 10.7524/AJE.1673-5897.20230106002
shu
Shen Hongyan, Han Dongxu, Yang Lei, Li Shuangjiang, Sun Haoyu, Tang Liang. Regulation of External Carbon Source on Antibiotic-induced Hormetic Effect in Escherichia coli[J]. Asian journal of ecotoxicology, 2023, 18(5): 156-164. doi: 10.7524/AJE.1673-5897.20230106002
Citation: Shen Hongyan, Han Dongxu, Yang Lei, Li Shuangjiang, Sun Haoyu, Tang Liang. Regulation of External Carbon Source on Antibiotic-induced Hormetic Effect in Escherichia coli[J]. Asian journal of ecotoxicology, 2023, 18(5): 156-164. doi: 10.7524/AJE.1673-5897.20230106002
shu

外加碳源对抗生素诱导大肠杆菌Hormesis效应的调控作用

  • 1. 河北科技大学环境科学与工程学院，石家庄 050018;

  • 2. 河北省药物分子化学重点实验室，石家庄 050018;

  • 3. 河北化工医药职业技术学院质量检测与管理系，石家庄 050026;

  • 4. 上海大学有机复合污染控制工程教育部重点实验室，上海 200444

    • 作者简介: 沈洪艳(1971-),女,博士,教授,研究方向为污染物环境行为及效应、环境影响评价及规划,E-mail:1178611805@qq.com
    通讯作者: 孙昊宇,E-mail:sunhaoyu2021@shu.edu.cn; 
  • 基金项目:

    国家自然科学基金(42277270，22006116)；河北省化工行业挥发性有机物检测与治理技术创新中心开放课题基金(ZXJJ20210404)

  • 中图分类号: X171.5

Regulation of External Carbon Source on Antibiotic-induced Hormetic Effect in Escherichia coli

  • 1. School of Environmental Science and Engineering, Hebei University of Science and Technology, Shijiazhuang 050018, China;

  • 2. Hebei Provincial Key Laboratory of Medicinal Molecular Chemistry, Shijiazhuang 050018, China;

  • 3. Department of Quality Inspection and Management, Hebei Chemical ＆ Pharmaceutical College, Shijiazhuang 050026, China;

  • 4. Ministry of Education Key Laboratory of Organic Compound Pollution Control Engineering, Shanghai University, Shanghai 200444, China

    • Corresponding author: Sun Haoyu, sunhaoyu2021@shu.edu.cn ; 
  • Fund Project:

  • 摘要: 抗生素对细菌通常表现出“低促高抑”的Hormesis效应，这显著影响了抗生素的生态风险评估。目前关于抗生素诱导细菌Hormesis效应的研究多集中于单一碳源条件，针对多种碳源共存条件的相关研究还十分有限。因此，为进一步探究外加碳源对抗生素诱导细菌Hormesis效应的影响，本文以大肠杆菌(Escherichia coli, E. coli)为受试生物，在外加不同浓度葡萄糖的Mueller-Hinton培养基中测定了盐酸四环素(tetracycline hydrochloride, TCH)和2(5H)-呋喃酮(2(5H)-furanone, 2F)2种抗生素单一及联合暴露对E. coli生长的毒性效应，并分析了外加葡萄糖与抗生素对E. coli生长的交互效应。结果表明，TCH和2F单一及联合暴露均能诱导E. coli产生Hormesis效应，随着外加葡萄糖浓度的升高，TCH、2F和TCH+2F在低浓度下对E. coli生长的促进作用逐渐增强，最大促进率分别由47.66%、9.08%、5.63%增加到158.65%、40.20%、21.30%；在高浓度下对E. coli生长的抑制作用逐渐减弱，EC50值分别从3.17E-05、1.62E-02、3.71E-03 mol·L-1增加到9.24E-05、4.10E-02、1.01E-02 mol·L-1；外加葡萄糖与抗生素对抑制E. coli生长的交互效应总体上呈拮抗作用，且随着外加葡萄糖浓度升高拮抗作用也随之增强，可以看出外加碳源能够降低抗生素的细菌毒性。本研究可为从外界营养条件角度更加全面评估抗生素的Hormesis效应及生态风险提供参考和数据支持。
    Abstract: Antibiotics usually trigger hormetic effects on bacteria that exhibit low-dose stimulation and high-dose inhibition, which significantly influence the ecological risk assessment of antibiotics. Currently, the studies regarding the hormesis effects of antibiotics on bacteria have been always conducted under the condition of single carbon source, while the related researches rarely consider the condition of multiple carbon sources. In this study, Escherichia coli (E. coli) was used as the test organism to further explore the influence of the added carbon source on the hormetic phenomena in bacteria induced by antibiotics. Under the culture conditions of Mueller-Hinton medium with added different concentrations of glucose, the single and combined toxicity of tetracycline hydrochloride (TCH) and 2(5H)-furanone (2F) was determined using the growth of E. coli as the endpoint, where the interaction effects between added glucose and antibiotics on E. coli growth were also analyzed. The results showed that TCH, 2F and their mixture could all induced the hormetic effects on E. coli. With the increase of the added glucose, the low-dose stimulatory effects of TCH, 2F and TCH+2F on E. coli growth gradually increased, while the high-dose inhibitory effects gradually weakened. The maximum stimulatory rates of TCH, 2F and TCH+2F increased from 47.66% to 158.65%, from 9.08% to 40.20%, and from 5.63% to 21.30%, respectively. The EC50 values of TCH, 2F and TCH+2F increased from 3.17E-05 mol·L-1 to 9.24E-05 mol·L-1, from 1.62E-02 mol·L-1 to 4.10E-02 mol·L-1, and from 3.71E-03 mol·L-1 to 1.01E-02 mol·L-1, respectively. The interaction effects between added glucose and antibiotics on E. coli growth were generally antagonism, which enhanced with the increase of added glucose. These results indicated that added carbon source could reduce the bacterial toxicity of antibiotics. This study can provide reference and data support for a more comprehensive assessment of antibiotics’ hormesis and their ecological risk from the perspective of external nutritional condition.
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  • 收稿日期:  2023-01-06
沈洪艳, 韩冬旭, 杨雷, 李双江, 孙昊宇, 唐量. 外加碳源对抗生素诱导大肠杆菌Hormesis效应的调控作用[J]. 生态毒理学报, 2023, 18(5): 156-164. doi: 10.7524/AJE.1673-5897.20230106002
引用本文: 沈洪艳, 韩冬旭, 杨雷, 李双江, 孙昊宇, 唐量. 外加碳源对抗生素诱导大肠杆菌Hormesis效应的调控作用[J]. 生态毒理学报, 2023, 18(5): 156-164. doi: 10.7524/AJE.1673-5897.20230106002
shu
Shen Hongyan, Han Dongxu, Yang Lei, Li Shuangjiang, Sun Haoyu, Tang Liang. Regulation of External Carbon Source on Antibiotic-induced Hormetic Effect in Escherichia coli[J]. Asian journal of ecotoxicology, 2023, 18(5): 156-164. doi: 10.7524/AJE.1673-5897.20230106002
Citation: Shen Hongyan, Han Dongxu, Yang Lei, Li Shuangjiang, Sun Haoyu, Tang Liang. Regulation of External Carbon Source on Antibiotic-induced Hormetic Effect in Escherichia coli[J]. Asian journal of ecotoxicology, 2023, 18(5): 156-164. doi: 10.7524/AJE.1673-5897.20230106002
shu

外加碳源对抗生素诱导大肠杆菌Hormesis效应的调控作用

    通讯作者: 孙昊宇,E-mail:sunhaoyu2021@shu.edu.cn; 
    作者简介: 沈洪艳(1971-),女,博士,教授,研究方向为污染物环境行为及效应、环境影响评价及规划,E-mail:1178611805@qq.com
  • 1. 河北科技大学环境科学与工程学院，石家庄 050018;
  • 2. 河北省药物分子化学重点实验室，石家庄 050018;
  • 3. 河北化工医药职业技术学院质量检测与管理系，石家庄 050026;
  • 4. 上海大学有机复合污染控制工程教育部重点实验室，上海 200444
  • 收稿日期:  2023-01-06
基金项目:

国家自然科学基金(42277270，22006116)；河北省化工行业挥发性有机物检测与治理技术创新中心开放课题基金(ZXJJ20210404)

摘要: 抗生素对细菌通常表现出“低促高抑”的Hormesis效应，这显著影响了抗生素的生态风险评估。目前关于抗生素诱导细菌Hormesis效应的研究多集中于单一碳源条件，针对多种碳源共存条件的相关研究还十分有限。因此，为进一步探究外加碳源对抗生素诱导细菌Hormesis效应的影响，本文以大肠杆菌(Escherichia coli, E. coli)为受试生物，在外加不同浓度葡萄糖的Mueller-Hinton培养基中测定了盐酸四环素(tetracycline hydrochloride, TCH)和2(5H)-呋喃酮(2(5H)-furanone, 2F)2种抗生素单一及联合暴露对E. coli生长的毒性效应，并分析了外加葡萄糖与抗生素对E. coli生长的交互效应。结果表明，TCH和2F单一及联合暴露均能诱导E. coli产生Hormesis效应，随着外加葡萄糖浓度的升高，TCH、2F和TCH+2F在低浓度下对E. coli生长的促进作用逐渐增强，最大促进率分别由47.66%、9.08%、5.63%增加到158.65%、40.20%、21.30%；在高浓度下对E. coli生长的抑制作用逐渐减弱，EC50值分别从3.17E-05、1.62E-02、3.71E-03 mol·L-1增加到9.24E-05、4.10E-02、1.01E-02 mol·L-1；外加葡萄糖与抗生素对抑制E. coli生长的交互效应总体上呈拮抗作用，且随着外加葡萄糖浓度升高拮抗作用也随之增强，可以看出外加碳源能够降低抗生素的细菌毒性。本研究可为从外界营养条件角度更加全面评估抗生素的Hormesis效应及生态风险提供参考和数据支持。

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