大气主要矿物颗粒/金黄色葡萄球菌模拟复合体特征分析
Characteristic analysis of atmospheric major mineral particles/ staphylococcus aureus simulation complex
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摘要: 大气环境污染日益复杂,大气中矿物颗粒与人体表面正常菌的复合体性质现在还不清楚,是否对人体具有危害还不得而知,大气主要矿物颗粒/金黄色葡萄球菌复合体特征的研究已经迫在眉睫.本文选取了3种矿物颗粒(石英、方解石、蒙脱石)和金黄色葡萄球菌,在成功制备矿物颗粒/金黄色葡萄球菌复合体的基础上,结合粒径分析仪、扫描电镜(SEM)、Zeta电位分析仪、傅里叶红外光谱仪(FTIR)等技术,对所制备的矿物/细菌复合体的粒径、形貌、表面电性及表面基团特征进行分析.粒径结果表明形成后的矿物/细菌复合体呈现粒径增大趋势;SEM结果表明,大量的金黄色葡萄球菌及其代谢产物粘附在矿物颗粒絮凝团表面;Zeta电位结果显示,复合体的电位值趋于矿物或者细菌的电位值;FTIR结果显示,矿物颗粒/金黄色葡萄球菌复合体中出现了来自金黄色葡萄球菌的特征峰,矿物颗粒其他基团出现了位移的现象.Abstract: Atmospheric environmental pollution is becoming more and more intricacy, the nature and hazard of complexes between mineral particles in the atmosphere and normal bacteria on the surface of the human body is still unclear, therefore, the characteristic research of major atmospheric mineral particles/Staphylococcus aureus complexes is imminent. In this paper, Three mineral particles (quartz, calcite and montmorillonite) and Staphylococcus aureus was selected to preparation complexes. On this basis, the particle size, morphology, surface electrical properties and surface group characteristics of mineral granule/Staphylococcus aureus complexes were analyzed through particle size analyzer, scanning electron microscope (SEM), Zeta potential analyzer and Fourier infrared spectrometer (FTIR). The following results were concluded. The particle size results showed that the particle size of mineral/bacterial complexes was bigger than the orginal mineral. Secondly, scanning electron microscope results showed that a large number of Staphylococcus aureus and its metabolites adhered to the surface of mineral floc. And then Zeta potential results showed that the potential value of the complex tends to the mineral or bacteria potential value. Finally, FTIR results showed that the characteristic peaks from Staphylococcus aureus appeared in mineral granules/Staphylococcus aureus complex, and the displacement phenomenon occurs in other groups of mineral particles. This study is expected to provide basic data support for solving the complex pollution problem in the atmospheric environment with practical significance.
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