摘要:
利用紫外和荧光法测定了荧蒽和NB06D经绿豆幼苗代谢前后的光谱变化.结果表明,经植物代谢后,荧蒽的紫外和荧光光谱的精细结构没有发生变化,而NB06D的紫外和荧光光谱的精细结构以及荧光的最大发射波长均发生了改变利用荧光法测定荧蒽和NB06D经植物代谢前后与DNA结合的结果表明,在浓度约为2.5×10-6mol·l-1时,荧蒽及其植物代谢提取物与浓度为5.0×10-7—5.0×10-5mol·l-1范围内的DNA不发生结合,而NB06D及其植物代谢提取物都能与上述浓度的DNA结合,但经植物代谢后,提取物与DNA的结合能力稍有减弱,该结果表明,荧蒽和母体化合物与DNA的结合能力不同,在试验期内,植物代谢对这两种在动物体试验中表现不同致癌性的多环芳烃与DNA间的结合没有明显的活化或去活化作用.
Abstract:
Metabolized by mung bean seedlings,the two kinds of polycyclic aromatic bydrocarbons,fluoranthene and chrysene,were extracted and separated from the plant.The UV spectrum and the fluo- rescence spectrum of the two kinds of chemicals had been determined before and after metabolized by plant,and the combination response of the two chemicals and their metabolites with DNA was determined by the method of fluorimetry.The result indicates that fluoranthene has no change to its fine structure of the UV and fluorescence spectrum before and after metabolized by the plant,but chrysene both change in it.In the concentration of 2.5�10-6mol·l-1,fluoranthene and its metabolites in the sane fluorescence intense with it have no combination with DNA which presented in the concentrations of 5.0�10-7-5.0�10-5mol·l-1,while chrysene can do it in the same conditions.Compared with chrysene,the combination capacity of chrysene metabolites with DNA has been weakened in a little.The results indicated that fluoranthene and chrysene in parent compound have different combination capacity with DNA.Metabolized by plant to these two kinds of polycyclic aromatic hydrocarbons,which have showed different carcinogenicity in animal test,has no displayed obvious activation or inactivation action.
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