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过氧化氢酶活性及活性抑制的紫外分光光度测定

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徐镜波, 袁晓凡, 郎佩珍. 过氧化氢酶活性及活性抑制的紫外分光光度测定[J]. 环境化学, 1997, 16(1): 73-76.
引用本文: 徐镜波, 袁晓凡, 郎佩珍. 过氧化氢酶活性及活性抑制的紫外分光光度测定[J]. 环境化学, 1997, 16(1): 73-76.
shu
Xu Jingbo, Yuan Xiaofan, Lang Peizhen. THE DETERMINATION OF ENZYMIC ACTIVITY AND ITS INHIBITION ON CATALASE BY ULTRAVIOLET SPECTROPHOTOMETRY[J]. Environmental Chemistry, 1997, 16(1): 73-76.
Citation: Xu Jingbo, Yuan Xiaofan, Lang Peizhen. THE DETERMINATION OF ENZYMIC ACTIVITY AND ITS INHIBITION ON CATALASE BY ULTRAVIOLET SPECTROPHOTOMETRY[J]. Environmental Chemistry, 1997, 16(1): 73-76.
shu

过氧化氢酶活性及活性抑制的紫外分光光度测定

  • 1.

    东北师范大学环境科学系, 长春130024

  • 基金项目:

    国家自然科学基金资助课题

THE DETERMINATION OF ENZYMIC ACTIVITY AND ITS INHIBITION ON CATALASE BY ULTRAVIOLET SPECTROPHOTOMETRY

  • 1.

    Department of Environmental Science, Northeast Normal University, Changchun, 130024

  • Fund Project:

  • 摘要: 在体内实验条件下,测定了鲤鱼肝脏过氧化氢酶的活性.实验结果表明,过氧化氢酶的紫外分光光度法是可靠的.活性抑制结果表明,对-二硝基苯(p-DNB)对鲤鱼肝脏过氧化氢酶均有明显的抑制作用,EC50值为0.0807mg·1-1.鲤鱼肝脏过氧化氢酶可作为一项指标用于生态毒理学研究.
    Abstract: Liver catalase (Ct) activities of carp were measured by ultraviolet spectropho-tometry and the results were reliable.The effect of p-DNB on Ct of carp liver were studied in Vivo.The results showed that p-DNB exhibited fairly strong inhibition to Ct of carp livers.A satisfactory straight line was obtained by plotting the probits of inhibition percentage of Ct against the logarithmic concentration of toxicants, The concentration causing 50% inhibtion of p-DNB to Ct carp liver was 0.087mg·l-1.It was proposed that the effect of toxicant on Ct could be used as an index of ecotoxicolgical assessment.
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  • 收稿日期:  1995-11-19
徐镜波, 袁晓凡, 郎佩珍. 过氧化氢酶活性及活性抑制的紫外分光光度测定[J]. 环境化学, 1997, 16(1): 73-76.
引用本文: 徐镜波, 袁晓凡, 郎佩珍. 过氧化氢酶活性及活性抑制的紫外分光光度测定[J]. 环境化学, 1997, 16(1): 73-76.
shu
Xu Jingbo, Yuan Xiaofan, Lang Peizhen. THE DETERMINATION OF ENZYMIC ACTIVITY AND ITS INHIBITION ON CATALASE BY ULTRAVIOLET SPECTROPHOTOMETRY[J]. Environmental Chemistry, 1997, 16(1): 73-76.
Citation: Xu Jingbo, Yuan Xiaofan, Lang Peizhen. THE DETERMINATION OF ENZYMIC ACTIVITY AND ITS INHIBITION ON CATALASE BY ULTRAVIOLET SPECTROPHOTOMETRY[J]. Environmental Chemistry, 1997, 16(1): 73-76.
shu

过氧化氢酶活性及活性抑制的紫外分光光度测定

  • 1. 东北师范大学环境科学系, 长春130024
  • 收稿日期:  1995-11-19
基金项目:

国家自然科学基金资助课题

摘要: 在体内实验条件下,测定了鲤鱼肝脏过氧化氢酶的活性.实验结果表明,过氧化氢酶的紫外分光光度法是可靠的.活性抑制结果表明,对-二硝基苯(p-DNB)对鲤鱼肝脏过氧化氢酶均有明显的抑制作用,EC50值为0.0807mg·1-1.鲤鱼肝脏过氧化氢酶可作为一项指标用于生态毒理学研究.

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