过氧化氢酶活性及活性抑制的紫外分光光度测定
THE DETERMINATION OF ENZYMIC ACTIVITY AND ITS INHIBITION ON CATALASE BY ULTRAVIOLET SPECTROPHOTOMETRY
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摘要: 在体内实验条件下,测定了鲤鱼肝脏过氧化氢酶的活性.实验结果表明,过氧化氢酶的紫外分光光度法是可靠的.活性抑制结果表明,对-二硝基苯(p-DNB)对鲤鱼肝脏过氧化氢酶均有明显的抑制作用,EC50值为0.0807mg·1-1.鲤鱼肝脏过氧化氢酶可作为一项指标用于生态毒理学研究.Abstract: Liver catalase (Ct) activities of carp were measured by ultraviolet spectropho-tometry and the results were reliable.The effect of p-DNB on Ct of carp liver were studied in Vivo.The results showed that p-DNB exhibited fairly strong inhibition to Ct of carp livers.A satisfactory straight line was obtained by plotting the probits of inhibition percentage of Ct against the logarithmic concentration of toxicants, The concentration causing 50% inhibtion of p-DNB to Ct carp liver was 0.087mg·l-1.It was proposed that the effect of toxicant on Ct could be used as an index of ecotoxicolgical assessment.
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Key words:
- carp liver /
- catalase (Ct) /
- enzymic activity /
- inhibition /
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