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酶催化荧光法测定大气水相中过氧化氢

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沈济, 赵倩雪. 酶催化荧光法测定大气水相中过氧化氢[J]. 环境化学, 1989, 8(5): 32-37.
引用本文: 沈济, 赵倩雪. 酶催化荧光法测定大气水相中过氧化氢[J]. 环境化学, 1989, 8(5): 32-37.
shu
Shen Ji, Zhao Qianxue. DETERMINATION OF HYDROGEN PEROXIDE IN ATMOSPHERIC PRECIPITATION BY ENZYME CATALYZED FLUOROMETRIC METHOD[J]. Environmental Chemistry, 1989, 8(5): 32-37.
Citation: Shen Ji, Zhao Qianxue. DETERMINATION OF HYDROGEN PEROXIDE IN ATMOSPHERIC PRECIPITATION BY ENZYME CATALYZED FLUOROMETRIC METHOD[J]. Environmental Chemistry, 1989, 8(5): 32-37.
shu

酶催化荧光法测定大气水相中过氧化氢

  • 1.

    中国科学院生态环境研究中心

DETERMINATION OF HYDROGEN PEROXIDE IN ATMOSPHERIC PRECIPITATION BY ENZYME CATALYZED FLUOROMETRIC METHOD

  • 1.

    Research Center for Eco-Environmental Sciences, Academia Sinica

  • 摘要: 本文报道的酶催化荧光技术测定大气水相中H2O2,可以在野外采集样品,经简单前处理后带回中心实验室测定。方法的最低检测限为2×10-8M,在1×10-7M至2×10-5M H2O2浓度范围内工作曲线呈线性,在2×10-6M和1×10-5M时的变异系数小于3.0%.若用流动注射方式工作,样品量小于1ml,2—3min 可以测定一个样品.
    Abstract: An enzyme catalyzed fluorometric method for the determination of hydrogen peroxide in atmospheric precipitation is reported. The determination is based on the formation of a stable fluorescent dimer by the reaction of hydrogen peroxide with p-hydroxyphenylacetic acid in the presence of peroxidase. The dimer can be made at the point of sampling location, and analyzed at a later time in a central laboratory. The minimum detection limit for H2O2 was found to be 2�10-8 M. The calibration curve was linear in the range of 1�10-7 to 2�10-5M of H2O2. Less then 1ml aqueous sample and 2-3 minutes are required for meas"ring one sample if a flow-injection system is used.
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  • 收稿日期:  1989-01-20
沈济, 赵倩雪. 酶催化荧光法测定大气水相中过氧化氢[J]. 环境化学, 1989, 8(5): 32-37.
引用本文: 沈济, 赵倩雪. 酶催化荧光法测定大气水相中过氧化氢[J]. 环境化学, 1989, 8(5): 32-37.
shu
Shen Ji, Zhao Qianxue. DETERMINATION OF HYDROGEN PEROXIDE IN ATMOSPHERIC PRECIPITATION BY ENZYME CATALYZED FLUOROMETRIC METHOD[J]. Environmental Chemistry, 1989, 8(5): 32-37.
Citation: Shen Ji, Zhao Qianxue. DETERMINATION OF HYDROGEN PEROXIDE IN ATMOSPHERIC PRECIPITATION BY ENZYME CATALYZED FLUOROMETRIC METHOD[J]. Environmental Chemistry, 1989, 8(5): 32-37.
shu

酶催化荧光法测定大气水相中过氧化氢

  • 1. 中国科学院生态环境研究中心
  • 收稿日期:  1989-01-20

摘要: 本文报道的酶催化荧光技术测定大气水相中H2O2,可以在野外采集样品,经简单前处理后带回中心实验室测定。方法的最低检测限为2×10-8M,在1×10-7M至2×10-5M H2O2浓度范围内工作曲线呈线性,在2×10-6M和1×10-5M时的变异系数小于3.0%.若用流动注射方式工作,样品量小于1ml,2—3min 可以测定一个样品.

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